Visual function in early age-related macular degeneration (AMD) were observed by contrast sensi-tivity(cs), macular threshold, FM 100-Hue test, dark adaptation, and the Amsler grid. All patients with early AMD achieved corrected visual acuity of 1.0 or better. The results revealed that early AMD showed one or more of following: abnormal macular light sensitivity (MLS), hue discripmination, CS, threshold of dark adaptation, and Amsler grid, comparing with those of the normal controls. These tests proved useful for the early diagnosis of AMD, and their sensitivity was found to be in the following descending order: MLS, FM100-hue, CS, and Amsler grid.

Five male and one female patients with IPVS caused by infarction of occipital cortex and optic radiations of brain were reported. The ages were in the range from 21 to 69 years. The evidence of diagnosis included sudden loss of visual acuity in both eyes, normal fundus, homonymous defect of visual field, infarction of brain in CT scan, without other positive signs of central nervous system or headache or eye pain before or at the time of attack. All the patients were treated by injection of Co- Anisodine Ⅰ and Ⅱ at acupuncture points with satisfactory effects. The therapy is safe, effective, and reliable. The clinical features and the treatment mechanism were disscused.

Thirty-six patients with normal vision in one eye coupled with advanced age-related macular degeneration(AMD)in the fellow eye,and 36 eyes with drusen in one eye coupled with macular drusen in the fellow eye,and 36 normal subjects(36 eyes) were measured by FM100-Hue test.All the tesed eyes had corrected vision of 1.0 or better and had drusen in the fundus.The results showed that the total errors score of FM100-Hue test of patients whose fellow eye suffered from advanced AMD compared with that of the patients whose fellow eye did not suffer from advanced AMD,difference between the two groups in the red and panchromatic regions was statistically significant(P

Objective:To study the effects of curcumin on the activity of ATPase and the mechanism of apoptosis in U-251 cell.Methods:U-251 cells were treated with 20,40,80,100?mol/L curcumin for 24 h and the growth inhibition rates of U-251 cells were measured with MTT method.Cell apoptosis was inspected with flow cytometry(FCM).The activities of ATPase were determined by colorimetry method.Results:Curcumin inhibited the proliferation of U-251 cells and induced apoptosis of U-251 cells.level of ATPase in U-251 plasma Membrane was low remarkably.Conclusion:Curcumin induced apoptosis and inhibited proliferation of U-251 cell via inhibition of activation of plasma Membrane ATPase.

Animals ; Biopsy, Needle ; Humans ; Kidney ; ultrastructure ; Mice ; Mice, Inbred BALB C ; Microscopy, Electron, Transmission ; methods ; Specimen Handling

Forty-two cases of acute lumbar sprain patients were treated by strongly stimulating Shuigou(GV 26).Of them,32 cases were cured,5 cases got obvious improvement and 5 cases obtained no effect.The effective rate was 88.1％.

Unilateral facet dislocation of the lower cervical spine is an important subgroup of cervical spine injuries,accounting for from 12％ to 16％ of all cervical spine injuries.Unilateral facet dislocation occurs the most commonly at the C5-6 level,and is closely related to the anatomic and morphometric features of the cervical facet.This injury has been attributed to combined flexion and rotation,but it remains unclear whether flexion or rotation is the component of greater importance.Its treatment includes nonoperative and operative measures but optimal treatment has not been established.It is generally accepted that a recommendable treatment protocol begins with closed reduction and proceeds depending on whether the closed reduction succeeds and traumatic disc herniation is present or not.

Population aging is a problem confronting many countries around the world and poses grim challenges to the conventional healthcare model.The measure proposal by WHO,the establishment of age-friendly community-based primary healthcare centers and medical institutions,is both practical and feasible,based on which many countries have formulated implementation frameworks.In China,it is of paramount importance to promote the development of age-friendly hospitals,especially geriatric specialty hospitals,rehabilitation hospitals and nursing homes,in the healthcare sector.Integrated care services offered by age-friendly hospitals can help reduce complications,improve care quality and patient satisfaction,and cut costs,benefiting elderly patients and their families and ultimately moving the healthcare reform forward.

Objective: To investigate the feature of crisis at asexual stages of chloroqine resistant strain (RC) of Plasmodium berghei . Methods: By means of optical microscope,transmission electron microscope and agarose electrophoresis, microscopic and ultramicroscopic architectures, and genomic DNA electrophoresis at asexual stages of the RC and chloroquine sensitive (N) strains of P. berghei with rising parasitemia and the RC strain of the parasite with declining parasitemia were observed in ICR mice. Results: Under microscope, different developmental stages asexual parasites with normal forms were found in blood smears from the mice infected by the N and RC strains with rising parasitemia, whereas there were a few of the asexual parasites most of which were contracted and vacuolated from the mice recovered from infection by the RC strain. Using electron microscope, intact cytoplasm membranes of the parasites paralleling with parasitophrous membrane of erythrocytes, nuclear laterad, obvious metabilic window and mitochondrions, ribosomes, endoplasmic reticulum in cytoplasm were recognized in erythrocytic stages of the RC and N strains with rising parasitemia. There were rhoptries in merozoites and food vacuoles in trophozoites of the N strain. Erythrocytic parasites of the RC strain from recovered mice with declining parasitemia were spherical or elliptical, with intact cytoplasm membrane, contracted nuclei and concentrated cytoplasm, without mitochondrion membranaceous structure and metabolic windows. The genomic DNA electrophoretogram of the RC strain parasites in the recovered mice showed a ladder pattern, which differed evidently from the parasites of the RC strain with rising parasitemia and the N strain which showed a single band. Conclusion: It is confirmed that crisis form of asexual stages of the RC strain is apoptosis.

Objective: To investigate virulence differences of the malaria parasites and responses of their hosts to the malaria infection between chloroquine sensitive (N) and resistant (RC) strains of Plasmodium berghei . Methods: Ultrastructural pathological alterations of liver, kidney and lungs of ICR mice infected with the N or the RC strains were investigated. Results: Congestion and more parasite infected red blood cells in hepatic sinusoids, renal interstitium, capillaries of the liver, kidney and lungs in late period of the infection with the N strain were observed. Necrosis of hepatic cells in liver, juxtaglomerular cells and epithelial cells of renal tubules in kidney, type Ⅰ and type Ⅱ alveolar cells in lungs occurred. Membranes of these cells were broken, resulting in cytoplasm missing and mitochondria swelling infected with the N strain. While degeneration of some hepatic cells of liver, epithelial cells of renal tubules, alveoli cells of lungs appeared during late period of the infection with the RC strain of P. berghei . It was observed that some hepatic cells recoved, the glomeruli basement membrane and mesangial cells contained electron dense deposits,alveoli septa were undergoing the proliferation and infiltration of lymphocytes, macrophages and neutrophilic granulocytes. Conclusion: The N strain is more virulent than the RC strain of P. berghei . Ultrastructures of the liver, kidney and lungs of the mice infected with the N strain show cellular necrosis, while those infected with the RC strain mainly reveal cellular degeneration.