OBJECTIVE: To prepare fumeisong cream and establish its quality control method. METHODS: Ingredients including carbamide, triamcinolone acetonide acetate, camphor, and laurocapram were mixed in an oil-in-water matrix for preparation of fumeisong cream. HPLC was adopted to determine the content of triamcinolone acetonide in the cream. RESULTS: The prepared cream was white in color, with test items all in line with specifications stipulated in 2005 version of China Pharmacopoeia. The linearity concentration range of triamcinolone acetonide was 2.06～20.6 ?g?mL-1(r=0.999 6) and the average recovery rate was 99.6% (RSD=1.0%,n=9). CONCLUSION: The cream is simple and feasible in preparation techniques, and its quality is stable and controllable.

Objective:To study cardiomyocyte apoptosis of viral myocarditis (VMC) mice,study the effect and significance of Tumor Necrosis Factor-? (TNF-?) to cardiomyocyte apoptosis in VMC.Methods:Our experiments studied cardiomyocyte apoptosis at 3、5、7、9、15、35 days after virus inoculation in VMC mice by ways of TUNEL and electron microscope.We studied the effect of TNF-? to cardiomyocyte apoptosis in VMC by the way of intervention with TNF-? and TNF-? mAb.Results:Apoptotic cardiomyocyte of VMC mice increased appaently compared with control mice.Cardiomyocyte apoptosis increased after TNF-? intervention,cardiomyocyte apoptosis reduced after TNF-? mAb intervention.Conclusion:Abnormal cardiomyocyte apoptosis was observed in VMC,cardiomyocyte apoptosis may has relation with the Pathogenesis of VMC.TNF-? can induce cardiomyocyte apoptosis in VMC.TNF-? induces cardiomyocyte apoptosis may has relation with its role in VMC.

Objective Propofol has profound influence on respiration. The purpose of this study was to evaluate the effect of propofol on respiratory mechanics during painless induced abortion. Methods Forty ASA Ⅰ women in early pregnancy (45-60 days) aged 17-52 yrs, weighing 42-80 kg undergoing induced abortion were enrolled in this study. Anesthesia was induced with intravenous 2.5 mg?kg-1 and maintained with intermittent i.v. boluses of propofol 30-50 mg. SpO2 and HR were monitored with Nellcor N-180 pulse oximeter. Respiratory mechanics was monitored with IFA-300 anemometer (TSI Co. USA) . The parameters measured included inspiratory / expiratory maximal air-flow velocity, inspiratory / expiratory mean air flow velocity, mean inspiratory and expiratory time, respiratory rate, inspiratory / expiratory air flow volume, dynamic inspiratory and expiraory airway pressure and indidence of apnea.Results SpO2 decreased significantly during maintenance of anesthesia with propofol. The mean and maximal inspiratory / expiratory airflow velocity and the inspiratory / expiratory airflow volume all decreased significantly during propofol anesthesia. The dynamic inspiratory / expiratory airway pressure significantly decreased during propofol anesthesia. The respiratory rate was significantly higher during propofol anesthesia while the mean inspiratory /expiratory time became shorter. Three patients developed apnea during induction of anesthesia with propofol (7.5%) . Spontaneous breathing returned within 1 min. Conclusion Spontaneous breathing is significantly depressed during propofol anesthesia in terms of respiratory mechanics. Care should be taken to maintain oxygenation and ventilation of the patient.

OBJECTIVE:To compare the therapeutic effects of taurine and catalin eye drops.METHODS:All the mice were divided into the normal control group,the cataract control group,the taurine group and the catalin eye drops group.Except the normal control group,the rest ones were used to establish the galactose cataract models and then received eyedrop administration everyday.The contents of taurine,glycin and glutamic acid in crystalline lens were measured by high perfor?mance liquid chromatography.RESULTS:The contents of amino-acids were not found lower in the taurine group as com?pared with the catalin eye drops group.CONCLUSION:Taurine shows the preventive and therapeutic effects on the experi?mental cataract,which can be comparable to that of catalin eye drops or even more advantageous.

Objective To observe the clinical efficacy of magnesium sulfate inhalation combined with budesonide and terbutaline sulfate nebulizer for the treatment of bronchiolitis.Methods Sixty children with bronchiolitis were randomly divided into observation group and control group with 30 cases in each group.Children in the control group received routine treatment and the observation group were inhaled with magnesium sulfate combining budesonide and terbutaline sulfate.Curative effects were evaluated in the aspects of the clinical symptoms,serum inflammatory factors and overall clinical efficacy.Results The symptoms in children including asthma,shortness of breath,coughing,wheezing and lung auscultation were significantly alleviated within a shorter period of time than that in the control group (P＜0.05).The serum levels of TNF-u,IL-6,IL-8 and TIgE were significantly reduced in both groups after the treatment (P＜0.05).But in the observation group,the alleviation of the symptoms was significantly greater than that in the control group (P＜0.05).The overall effective rate in the observation group and the control group was 93.3％ and 70.0％ respectively (P＜0.001).Conclusion The clinical efficacy of inhaling magnesium sulfate with budesonide and terbutaline sulfate to treat bronchiolitis is satisfactory and worth clinical application.

The paper presented the scheme of performance appraisal on rational administration of drugs and its outcomes against the background of separation of clinic from pharmacy reform.It found the proportion of medicine decreasing year by year;pass rate of prescriptions above 99％;drug cost per visit decreasing significantly;and the usage rate of antibiotics up to the national standards in a rational trend.Rational drug use has become an effective tool in overseeing physicians' rational drug use and ensuring patient safety.

BACKGROUND: Periprosthetic osteolysis and subsequent aseptic loosening are most common reasons forrevision after total joint replacements. Recently studies found that calcineurin/activated T cell nuclear factor (CN/NFAT) signaling pathway plays important roles in the pathogenic process of wear-induced inflammation and osteolysis. OBJECTIVE: To summarize CN/NFAT effects on wear particles-induced osteolysis, and to provide new ideas and newtheories for osteolysis-related diseases. METHODS: We retrieved PubMed for studies published from January 1980 to June 2016. The key words were CN/NFAT, CN/NFAT signaling pathways, wear particles, osteolysis, osteoclasts, osteoblast. Finally, 72 studies were analyzed and explored according to inclusion and exclusion criteria.RESULTS AND CONCLUSION: The pathogenesis of osteolysis involves the activation of macrophages by wear particles and the release of various proinflammatory cytokines. Bone is continuously renewed through a dynamic balance between bone resorption and formation and is the fundamental basis for maintaining normal bone mass andarchitecture by osteoclasts and osteoblasts. In this process, CN/NFAT signaling pathway is the signal transduction pathway involved in the differentiation of Ca2+ and nuclear factor kappa B receptor ligand. Osteoclasts play a significant role in both physiological and pathological bone resorption, and receptor activator of nuclear factor-κB ligand is the key cytokine that induces osteoclastogenesis.

Objective:To optimize the formula and preparation process of prepare sorafenib dry suspension and detect the content by HPLC. Methods:The influence of single factor including different fillers, suspending agents, adhesives and disintegrants on the sedimentation rate, redispersibility and drying shrinkage of sorafenib dry suspension was observed. Orthogonal design was used to opti-mize the formula and preparation process of the suspension, and HPLC was used to determine the content of sorafenib. The chromato-graphic column was Inertsil ODS-3 (250 mm × 4. 6 mm, 5μm), the mobile phase was 20 mmol·L-1 ammonium acetate buffer-aceto-nitrile(28 ∶72), the detection wavelength was 266 nm, the flow rate was 1. 0 ml·min-1, the column temperature was 40℃, and the sample size was 20 μl. Results:The optimal formula and preparation process were as follows:sucrose (48%) was used as the filler, xanthan gum (28%) and CMC-Na (12%) were the suspending agents, MCC (10%) was used as the disintegrant, and 6% PVP (in 50% ethanol) was the adhesive. The linear range of sorafenib was 1-100 μg·ml-1(r=0.999 8), and the recovery was 98.96%(RSD=0. 75%, n=9). Conclusion:The optimal formula and preparation process are repeatable. The HPLC method is simple and specific, which can be used to determine sorafenib.

OBJECTIVE: To prepare Baicalin dispersible tablets and establish a quality control method for this preparation. METHODS: The formula of Baicalin dispersible tablets was optimized by orthogonal experiment with disintegrating time as index. The content and dissolution rate of the tablets were determined by HPLC. RESULTS: The optimized formula was as follows: the contents of crospovidone, sodium starch glycolate and amylum pregelatinisatum were 40%, 6% and 2%, respectively. The dispersible tablets prepared in accordance with the optimize formula could disintegrate completely within 3 minutes and its dissolution rate could reach beyond 90% in 15 minutes. CONCLUSION: The preparation process is simple and feasible, and the prepared Baicalin dispersible tablets had a rapid disintegration and high dissolution rate.

Tuberculosis of bone and joint most commonly occurs in spine, and the main treatment method is anterior debridement, bone grafting and internal fixation. Autologous bone is still considered the "gold standard" when the anterior reconstruction of spine after debridement is needed. Allogeneic bone, artificial bone and artificial intervertebral support bodies such as titanium mesh and some biological materials also have a broad clinical application, but still can not completely replace the autologous bone because of their defects. In the aspect of internal fixation, anterior fixation has become a conventional method for the patients who suffer from active spinal tuberculosis and need anterior debridement of the focus, because this method can provide good support. This review summarizes the present situation of choice for supporting and bone grafting material for anterior reconstruction after debridement of spinal tuberculosis.