OBJECTIVE: To prepare fumeisong cream and establish its quality control method. METHODS: Ingredients including carbamide, triamcinolone acetonide acetate, camphor, and laurocapram were mixed in an oil-in-water matrix for preparation of fumeisong cream. HPLC was adopted to determine the content of triamcinolone acetonide in the cream. RESULTS: The prepared cream was white in color, with test items all in line with specifications stipulated in 2005 version of China Pharmacopoeia. The linearity concentration range of triamcinolone acetonide was 2.06～20.6 ?g?mL-1(r=0.999 6) and the average recovery rate was 99.6% (RSD=1.0%,n=9). CONCLUSION: The cream is simple and feasible in preparation techniques, and its quality is stable and controllable.

Objective To explore the effect of the different doses of oxytocin(OXT) on stress model aggressive behaviour in Wistar rats.Methods According to random number table,40 pregnant rats were divided into experimental stress groups (A, B, C) and control group(D) with 10 samples in each group.Before forced swimming test, rats in experimental groups (A, B, C)were given OXT via intraperitoneal injection with 0.3 mg/kg, 1.0 mg/kg,2.0 mg/kg;rats in group D were given equal volume of normal saline in the same way.At the 3rd day all rats were tested with maternal aggression,including mothers' following indices as total aggression times,biting times,pinning times,attack latency and duration of attack.Elevated plus maze (EPM) test was applied to analyze the behavioral changes.Results In terms of the duration of attack and attack latency,the indexes of group A and group D were significantly different from relevant data ((147.60±23.92) s and(79.70±9.88) s, P＜0.05), while other indexes had no difference(P＞0.05).The duration of attack and attack latency had a significant difference ((3.10±0.87) times, (13.60±5.14)times, (91.30±9.74)s and(167.20±30.02) s, P＜0.01) between group B and group D except total aggression times(P＞0.05).Group C and group D in given indexes had significant differences showing (6.40± 1.34) times, (15.10±4.35) times, (23.70±3.46) times, (49.80± 5.53) s and (215.60± 39.55) s (P＜0.01).Group A and group B in given indexes had significant differences (P＜ 0.05);and group B and group C had a significant difference (P＜0.01)except pinning times(P＞0.05).In EPM ,the open arm entry ratio and the open arm time ratio of both group A and group D were significantly different from respective percentage (0.47±0.13) and (0.13±0.05) (P＜0.01).The significant difference was found in group B and group D(P＜0.01);group A and group B had a statistical difference(P＜0.05);there was no difference in both groups in open arm time ratio(P＞0.05).Conclusion Oxytocin potentially mediates maternal aggression by attenuating stress, and improves the intimate bonding between mother and pups,which means dose dependency of OXT in this process.

Objective To establish the quality standard of Huangqi-Changyu'an capsule. Method Thin layer chromatography was used to identify psoralen, evodia mtaecarpa, and nutmeg in Huangqi-changyu'an capsule. HPLC was used to determine the content of psoralen. A Hypeisil BDS C18 column(200 mm×4.6 mm,5 μm) was used with mobile phase consisted of acetonitrile-water (30:70) at the rate of 1 ml/min. The detection wavelength was set at 245nm with the column at room temperature. Results The liner range of psoralen was 3.572~53.58 μg/ml (r=0.999 9) with average recoveries of 99.04%, and the RSD was 0.8%. The liner range of isopsoralen was 3.796~56.94 μg/ml (r=0.999 9) with average recoveries of 99.20%, and the RSD was 0.6%. Conclusion This method is sentivive, accurate and rapid for the quality control of Huangqi-Changyu'an capsule.

OBJECTIVE:To investigate the therapeutic efficacy of celecoxib in the treatment of knee osteoarthritis and its ef-fects on patin degree. METHODS:One hundred and twenty cases of knee osteoarthritis were selected from our hospital during Jan. 2014 to Jan. 2016,and then divided into control group and observation group according to therapy plan,with 60 cases in each group. Control group was given Ibuprofen sustained-release capsule 0.3 g,bid;observation group was additionally given lornoxi-cam 8 mg,bid,on the basis of control group. Both groups were treated for 4 weeks. VAS score was compared between 2 groups before and after treatment,and clinical efficacy and the occurrence of ADR were observed in 2 groups. RESULTS:There was no statistical significance in VAS score between 2 groups before treatment(P>0.05). After treatment,VAS score of 2 groups were de-creased significantly,and the observation group was significantly lower than the control group,with statistical significance (P<0.05). Clinical response rate of observation group was 95.0%,which was significantly higher than 75.0% of control group,with statistical significance (P<0.05). The incidence of ADR was 3.33% in observation group,which was significantly lower than 11.67% of control group,with statistical significance(P<0.05). CONCLUSIONS:Lornoxicam is effective for knee osteoarthritis and can significantly improve pain with good safety.

Objective To observe the clinical efficacy of magnesium sulfate inhalation combined with budesonide and terbutaline sulfate nebulizer for the treatment of bronchiolitis.Methods Sixty children with bronchiolitis were randomly divided into observation group and control group with 30 cases in each group.Children in the control group received routine treatment and the observation group were inhaled with magnesium sulfate combining budesonide and terbutaline sulfate.Curative effects were evaluated in the aspects of the clinical symptoms,serum inflammatory factors and overall clinical efficacy.Results The symptoms in children including asthma,shortness of breath,coughing,wheezing and lung auscultation were significantly alleviated within a shorter period of time than that in the control group (P＜0.05).The serum levels of TNF-u,IL-6,IL-8 and TIgE were significantly reduced in both groups after the treatment (P＜0.05).But in the observation group,the alleviation of the symptoms was significantly greater than that in the control group (P＜0.05).The overall effective rate in the observation group and the control group was 93.3％ and 70.0％ respectively (P＜0.001).Conclusion The clinical efficacy of inhaling magnesium sulfate with budesonide and terbutaline sulfate to treat bronchiolitis is satisfactory and worth clinical application.

Objective:To optimize the formula and preparation process of prepare sorafenib dry suspension and detect the content by HPLC. Methods:The influence of single factor including different fillers, suspending agents, adhesives and disintegrants on the sedimentation rate, redispersibility and drying shrinkage of sorafenib dry suspension was observed. Orthogonal design was used to opti-mize the formula and preparation process of the suspension, and HPLC was used to determine the content of sorafenib. The chromato-graphic column was Inertsil ODS-3 (250 mm × 4. 6 mm, 5μm), the mobile phase was 20 mmol·L-1 ammonium acetate buffer-aceto-nitrile(28 ∶72), the detection wavelength was 266 nm, the flow rate was 1. 0 ml·min-1, the column temperature was 40℃, and the sample size was 20 μl. Results:The optimal formula and preparation process were as follows:sucrose (48%) was used as the filler, xanthan gum (28%) and CMC-Na (12%) were the suspending agents, MCC (10%) was used as the disintegrant, and 6% PVP (in 50% ethanol) was the adhesive. The linear range of sorafenib was 1-100 μg·ml-1(r=0.999 8), and the recovery was 98.96%(RSD=0. 75%, n=9). Conclusion:The optimal formula and preparation process are repeatable. The HPLC method is simple and specific, which can be used to determine sorafenib.

Chimeric antigen receptor T cells (CAR-T), one of the most promising cancer immunotherapy, has attracted much attention in the 57th American Society of Hematology (ASH) annual meeting. CAR-T therapy has obtained significant effect on leukemia and lymphoma and the latest research results are also inspiring in the 57th ASH annual meeting. It is an important task that how to combine CAR-T therapy with the traditional methods of treatment and the immune checkpoint blocking antibodies and small-molecule-targeted drugs to achieve the best effect. This paper will review the progress of CAR-T in the treatment of hematologic malignancies.

Diabetic retinopathy (DR) is a common cause of blindness,its occurrence and development are the synergic results of multiple factors.Current studies suggest that inflammation and inflammatory factor has an important role in the pathogenesis of DR.The occurrence and development of DR are closely related with interleukins,intercellular adhesion molecules,hasten factors,tumor necrosis factor,C-reactive protein etc.Mesenchymal stem cells (MSCs) are pluripotent cells derived from the mesoderm and have multiple differentiation potentials,and anti-inflammatory and immunosuppressive function.Recent studies shown that MSCs transplantation can protect damaged retina by inflammatory regulation,which become a new research direction for DR treatment.

With the further study on heterogeneity and molecular mechanism of diffuse large B-cell lymphoma (DLBCL),many novel targeted drugs have been developed,which also showed clinical benefit in early-stage studies.Therapeutic targets for DLBCL mainly include the surface antigens of B lymphocyte,B cell receptor signaling and the cellular microenvironment.Several new clinical trials about targeted therapy for DLBCL had been reported in the 55th ASH annual meeting,and the results were encouraging.The advances in this field will be summarized in this paper based on the new reports in the 55th ASH annual meeting.

Serious market failure, as a result of insufficient incentive, exists in the orphan drugs market. Through legislation, the United States designed and established its owns systematic and linked orphan drugs incentive mechanism, which direct sat research, definition, clinical trials and approval of orphan drugs, so as to change the supply and demand status of orphan drugs. Such incentive mechanism erables the United States possess the most ap-proved orphan drugs in the world, and effectively relieves the problem of market failure of the orphan drugs. The ex-perience of the United States provides beneficial reference for the establishment and positive development of orphan drugs incentive mechanism in China.