A quantitative HPLC-DAD method was developed for simultaneous determination of N-trans-p-coumaroyloctopamine and N-trans-p-coumaroyltyramine in Solani Melongenae Radix from different cultivation regions in China The separation was performed on an Agilent Eclipse XDB C18 column (4.6 mm x 250 mm, 5 microm) at 30 degrees C with a gradient elution of methanol and 0.1% formic acid in water as mobile phase. The flow rate was set at 1.0 mL x min(-1) and the detection wavelength was 300 nm. The calibration curves of N-trans-p-coumaroyloctopamine and N-trans-p-coumaroyltyramine were linear over the ranges of 2.84-68.16, 3.10-74.40 mg x L(-1), and the average recoveries (n = 9) were 99.30% and 102.8%, respectively. The developed method was successfully applied for the analysis of sixteen samples from different cultivation regions in China, which indicated that the method is simple, rapid, accurate, and reliable for quality evaluation of Solani Melongenae Radix.
Amides ; analysis ; China ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; Plant Roots ; chemistry ; Solanaceae ; chemistry ; classification

Ten known phenolic compounds including [4]-gingerol (1), [6]-gingerol (2), [10]-gingerol (3), (3S,5S)-3,5-dihydroxy-1-(4-hydroxy-3-methoxyphenyl) decane (4), (3R,5S) -3, 5-dihydroxy-1-(4-hydroxy-3-methoxyphenyl) decane (5), [6]-shogaol (6), [10]-shogaol (7), gingerenone A (8), hexahydrocurcumin (9), and (3R,5R)-3,5-dihydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl) heptane (10), and seven amides including piperine (11), isochavicine (12), isopiperine (13), N-trans-p-coumaroyl tyramine (14), N-trans-feruloyl tyramine (15), N-trans-p-coumaroyl octopamine (16), N-trans-feruloyl octopamine (17), were isolated and identified from the roots of Lycianthes marlipoensis. Compounds 1-13 and 17 were isolated from the genus Lycianthes for the first time.
Amides ; chemistry ; isolation & purification ; Chromatography ; methods ; Magnetic Resonance Spectroscopy ; methods ; Phenols ; chemistry ; isolation & purification ; Plant Extracts ; chemistry ; isolation & purification ; Plant Roots ; chemistry ; metabolism ; Solanaceae ; chemistry ; metabolism

AIMTo modify the structure of dehydroepiandrosterone (DHEA).

METHODSUsing hairy root cultures of Anisodus tanguticus to perform biotransformation of DHEA, using chromatographic and spectral techniques to isolate and identify the products.

RESULTS(1) The MS medium without plant hormone was suitable for the growth of the hairy root. (2) DHEA was converted into five products: androst-4-ene-3, 17-dione (I); 6alpha-hydroxyandrost-4-ene-3, 17-dione (II); 6alpha, 17beta-dihydroxyandrost-4-ene-3-one (III); androst-4-ene-3, 6, 17-trione (IV) and 17beta-hydroxyandrost-4-ene-3-one (V).

CONCLUSIONIt is the first time to use hairy root cultures of Anisodus tanguticus for the biotransformation of DHEA and five DHEA-related compounds were obtained.

Androstenedione ; chemistry ; isolation & purification ; Androstenes ; chemistry ; isolation & purification ; Biotransformation ; Culture Media ; Dehydroepiandrosterone ; chemistry ; metabolism ; Molecular Structure ; Plant Roots ; metabolism ; Plants, Medicinal ; metabolism ; Solanaceae ; metabolism ; Tissue Culture Techniques

AIMTo identify anisodine and its metabolites in rat plasma after ingestion of anisodine by combining liquid chromatography and tandem mass spectrometry (LC-MS(n)).

METHODSPlasma samples from rats after a single orally administration of 20 mg anisodine were added with methanol to precipitate protein. Then, it was analyzed by LC-MS(n). Identification and structural elucidation of the metabolites were performed by comparing their changes in molecular masses, retention-times and full scan MS(n) spectra with those of the parent drug and blank plasma.

RESULTSThe results revealed that the parent drug and its four metabolites (norscopine, scopine, hydroxyanisodine, N-oxide anisodine) existed in rat plasma.

CONCLUSIONThis method is sensitive, rapid, simple, and it is suitable for the rapid identification of drug and its metabolits.

Administration, Oral ; Animals ; Chromatography, Liquid ; methods ; Plants, Medicinal ; chemistry ; Rats ; Rats, Wistar ; Scopolamine Derivatives ; isolation & purification ; metabolism ; Sensitivity and Specificity ; Solanaceae ; chemistry ; Tandem Mass Spectrometry ; methods

OBJECTIVE: To separate and determine scopolamine from the food in a poisoning case by GC/MS. METHODS: The scopolamine was determined by GC/MS/El used CP5860(CP-sil8CB) column (30 mx 0.25 mmx 0.33 microm) with liquid- liquid extraction. RESULTS: The deny scopolamine was found in the case sample, and the chromatographic separation of the peaks is fine. CONCLUSION The method is accurate and reliable.
Foodborne Diseases/etiology* ; Forensic Medicine/methods* ; Gas Chromatography-Mass Spectrometry/methods* ; Hallucinations/diagnosis* ; Humans ; Male ; Psychoses, Substance-Induced/etiology* ; Scopolamine/poisoning* ; Sensitivity and Specificity ; Solanaceae/chemistry* ; Solvents/chemistry*

AIMTo study the chemical constituents of Lycianthes biflora.

METHODSColumn chromatography was used to separate the chemical constituents. IR, MS, 1HNMR, 13CNMR and 2D-NMR technique were used to determine the structures of the isolated constituents.

RESULTSFive compounds were isolated from this plant. Their structures were identified to be bifloride A (1), N-trans-cinnamoyltyramine (2), liquiritigenin (3), N-trans-p-coumaroyloctopamine (4), 1-O-beta-D-glucopyranosyl-2-N-2'-hydroxypalmitoyl-sphinga-4- trans-8-trans-dienine (5).

CONCLUSIONCompounds 1 and 2 are new compounds, the others were isolated from this plant for the first time. Compound 2 showed inhibitory effects on P-388.

4-Butyrolactone ; analogs & derivatives ; chemistry ; isolation & purification ; Animals ; Antineoplastic Agents, Phytogenic ; chemistry ; isolation & purification ; Cinnamates ; chemistry ; isolation & purification ; Leukemia P388 ; pathology ; Mice ; Molecular Structure ; Plants, Medicinal ; chemistry ; Solanaceae ; chemistry ; Tumor Cells, Cultured ; Tyramine ; analogs & derivatives ; chemistry ; isolation & purification

OBJECTIVETo evaluate the antifeedant, insecticidal and growth inhibition activities of Solanum pseudocapsicum (S. pseudocapsicum) seed extracts against Spodoptera litura (S. litura) and Helicoverpa armigera (H. armigera).

METHODSHexane, diethyl ether, dichloromethane and ethyl acetate seed extracts were prepared and tested for antifeedant, insecticidal and growth inhibitory activities against fourth instar larvae of S. litura and H. armigera.

RESULTSEthyl acetate extract showed promising antifeedant and insecticidal activities against S. litura and H. armigera. Percentage of deformed larvae, pupae and adults were maximum in treatment of ethyl acetate extract. Percentage of successful adult emergence was deteriorated by seeds on extract treated larvae.

CONCLUSIONSEthyl acetate extracts of S. pseudocapsicum, showed higher efficiency of antifeedant, insecticidal and growth inhibition activities. Hence, it can be used to controll agricultural insect pests, S. litura and H. armigera.

Animals ; Humans ; Insecticides ; pharmacology ; Larva ; drug effects ; growth & development ; Lethal Dose 50 ; Moths ; drug effects ; growth & development ; Pest Control ; Plant Extracts ; pharmacology ; Solanaceae ; chemistry ; Spodoptera ; drug effects ; growth & development