Purpose: This study aimed to identify the most delirium assessment tool with high predictive validity for elderly patients with stroke. Methods: This was a prospective observational study. The subjects were 219 stroke patients aged 60 years or older admitted to the neurology ward of a general hospital and data collection was conducted from August 2022 to February 2023. The collected data were analyzed using descriptive statistics, x 2 -test, independent t-test, and Fisher's exact test. The predictive validity of the Delirium Observation Screening Scale (DOS), the Nursing Delirium Screening Scale(Nu-DESC), and the 4'A's test (4AT) were assessed based on the sensitivity, specificity, positive and negative predictive values, and area under the receiver operating characteristic curve (AUC). Results: Of the 38 patients screened for delirium using delirium assessment tools, 32 (14.6%) were diagnosed with delirium by a neurologist. The sensitivity, specificity, positive predictive value, and negative predictive value were 84.4%, 91.1%, 71.1%, and 97.2% for DOS; 93.8%, 96.8%, 83.3%, and 98.9% for Nu-DESC; and 100.0%, 96.8%, 84.2%, and 100.0% for 4AT, respectively. The AUC was shown to be 0.89 for the DOS, 0.95 for the Nu-DESC, and 0.98 for the 4AT. Conclusion The results indicate that the 4AT was the most valid delirium assessment tool for elderly patients with stroke. Therefore, the active use of 4AT by nurses to screen for delirium of elderly stroke patients in clinical settings can contribute to the prevention and early management of delirium.

Purpose: This study aimed to identify the most delirium assessment tool with high predictive validity for elderly patients with stroke. Methods: This was a prospective observational study. The subjects were 219 stroke patients aged 60 years or older admitted to the neurology ward of a general hospital and data collection was conducted from August 2022 to February 2023. The collected data were analyzed using descriptive statistics, x 2 -test, independent t-test, and Fisher's exact test. The predictive validity of the Delirium Observation Screening Scale (DOS), the Nursing Delirium Screening Scale(Nu-DESC), and the 4'A's test (4AT) were assessed based on the sensitivity, specificity, positive and negative predictive values, and area under the receiver operating characteristic curve (AUC). Results: Of the 38 patients screened for delirium using delirium assessment tools, 32 (14.6%) were diagnosed with delirium by a neurologist. The sensitivity, specificity, positive predictive value, and negative predictive value were 84.4%, 91.1%, 71.1%, and 97.2% for DOS; 93.8%, 96.8%, 83.3%, and 98.9% for Nu-DESC; and 100.0%, 96.8%, 84.2%, and 100.0% for 4AT, respectively. The AUC was shown to be 0.89 for the DOS, 0.95 for the Nu-DESC, and 0.98 for the 4AT. Conclusion The results indicate that the 4AT was the most valid delirium assessment tool for elderly patients with stroke. Therefore, the active use of 4AT by nurses to screen for delirium of elderly stroke patients in clinical settings can contribute to the prevention and early management of delirium.

Purpose: This study aimed to identify the most delirium assessment tool with high predictive validity for elderly patients with stroke. Methods: This was a prospective observational study. The subjects were 219 stroke patients aged 60 years or older admitted to the neurology ward of a general hospital and data collection was conducted from August 2022 to February 2023. The collected data were analyzed using descriptive statistics, x 2 -test, independent t-test, and Fisher's exact test. The predictive validity of the Delirium Observation Screening Scale (DOS), the Nursing Delirium Screening Scale(Nu-DESC), and the 4'A's test (4AT) were assessed based on the sensitivity, specificity, positive and negative predictive values, and area under the receiver operating characteristic curve (AUC). Results: Of the 38 patients screened for delirium using delirium assessment tools, 32 (14.6%) were diagnosed with delirium by a neurologist. The sensitivity, specificity, positive predictive value, and negative predictive value were 84.4%, 91.1%, 71.1%, and 97.2% for DOS; 93.8%, 96.8%, 83.3%, and 98.9% for Nu-DESC; and 100.0%, 96.8%, 84.2%, and 100.0% for 4AT, respectively. The AUC was shown to be 0.89 for the DOS, 0.95 for the Nu-DESC, and 0.98 for the 4AT. Conclusion The results indicate that the 4AT was the most valid delirium assessment tool for elderly patients with stroke. Therefore, the active use of 4AT by nurses to screen for delirium of elderly stroke patients in clinical settings can contribute to the prevention and early management of delirium.

Purpose: This study aimed to identify the most delirium assessment tool with high predictive validity for elderly patients with stroke. Methods: This was a prospective observational study. The subjects were 219 stroke patients aged 60 years or older admitted to the neurology ward of a general hospital and data collection was conducted from August 2022 to February 2023. The collected data were analyzed using descriptive statistics, x 2 -test, independent t-test, and Fisher's exact test. The predictive validity of the Delirium Observation Screening Scale (DOS), the Nursing Delirium Screening Scale(Nu-DESC), and the 4'A's test (4AT) were assessed based on the sensitivity, specificity, positive and negative predictive values, and area under the receiver operating characteristic curve (AUC). Results: Of the 38 patients screened for delirium using delirium assessment tools, 32 (14.6%) were diagnosed with delirium by a neurologist. The sensitivity, specificity, positive predictive value, and negative predictive value were 84.4%, 91.1%, 71.1%, and 97.2% for DOS; 93.8%, 96.8%, 83.3%, and 98.9% for Nu-DESC; and 100.0%, 96.8%, 84.2%, and 100.0% for 4AT, respectively. The AUC was shown to be 0.89 for the DOS, 0.95 for the Nu-DESC, and 0.98 for the 4AT. Conclusion The results indicate that the 4AT was the most valid delirium assessment tool for elderly patients with stroke. Therefore, the active use of 4AT by nurses to screen for delirium of elderly stroke patients in clinical settings can contribute to the prevention and early management of delirium.

The purpose of this study was to investigate nutrition knowledge, dietary attitude and a food habits of middle school students. The study was carried out through questionnaire. The subjects were 431 middle school students (boys 298, girls 133) in Chonbuk area. In nutrition knowledge, there were no significant differences in total scores between boys and girls. However, the girls showed higher score in knowledge of weight control than boys did (p < 0.05). In dietary attitude, there were significant differences in attitude of "balanced meal (p < 0.05)", "sufficient protein intake (p < 0.01)", "food diversity (p < 0.001)" and "overeating (p < 0.01)" between boys and girls. The boys showed better dietary attitudes than the girls did. In food habits, there were significant differences in the rate of skipping breakfast (p < 0.05), the rates of skipping dinner (p < 0.01), the frequency of snacks (p < 0.05), the type of snacks (p < 0.05) between boys and girls. The girls showed higher rates of skipping a meal and frequency of snacks than the boys did. It suggests that gender should be considered for an effective and practical nutrition education for middle school students to improve dietary attitudes and food habits.
Breakfast ; Education ; Female ; Food Habits* ; Humans ; Jeollabuk-do* ; Meals ; Snacks

Cellular cardiomyoplasty has recently emerged as a potential new treatment of ischemic heart disease. Combining cellular cardiomyoplasty with gene therapy using myogenic transcription factor might facilitate myocardial regeneration. In this study, we engineered H9c2, L6 using plasmid vector to overexpress the transcription factor MEF2c, Nkx2.5 involved in cardiomyogenesis. We investigated 1) formation of intercellular junction in mono-culture and co-culture with cardiomyocyte for functional and structural synchronous contraction after transplantation, 2) differentiation into cardiomyocyte, 3) resistance to hypoxic condition. Each cell overexpressing MEF2 and Nkx2.5 was generated by gene transfection and clonal selection. CO-culture was performed that each cell line added over cultured cardiomyocyte. H9c2-MEF2c and H9c2-Nkx2.5 became long, spindle shape like cardiomyocyte. Troponin T, cardiac specific marker, was found spot-like pattern in H9c2-Nkx2.5. However, co-culture with cardiomyocyte did not induce differentiation all kinds of cells into cardiomyocyte. Connexin43, which is gap junction marker was increased in H9c2-MEF2c, H9c2-Nkx2.5, L6-MEF2c and L6-Nkx2.5. Especially, co-culture with cardiomyocyte resulted in elevation of connexin43 levels more than monoculture. Ultrastructurally, formations of gap junction and desmosome were found apparently in L6-Nkx2.5. Long-standing, strong, regular and more frequent contraction were observed in cardiomyocyte co-cultured with H9c2-MEF2c, H9c2-Nkx2.5, L6-MEF2c, L6-Nkx2.5, respectively. Neverthless, any cell did not have active contraction itself, but passive movement except cardiomyocyte. H9c2-MEF2c, L6-MEF2c and L6-Nkx2.5 had resistance to hypoxia compared with other groups. These results suggested that co-culture and overexpressions of MEF2c and Nkx2.5 induced differentiation into cardiomyocyte and played an important role on intercellular junction formation and hypoxic resistance. This would be a promising source of cellular cardiomyoplasty. Therefore, much more research would be essential for clinical application of cellular cardiomyoplasty and this study would be a basic source for further study of MEF2c and Nkx2.5 in cellular cardiomyoplasty.
Animals ; Anoxia ; Cardiomyoplasty ; Cell Line ; Cell Transplantation ; Coculture Techniques* ; Connexin 43 ; Desmosomes ; Gap Junctions ; Genetic Therapy ; Intercellular Junctions ; Myoblasts ; Myoblasts, Cardiac* ; Myocardial Ischemia ; Myocytes, Cardiac* ; Plasmids ; Rats* ; Regeneration ; Transcription Factors ; Transfection ; Troponin T

BACKGROUND AND OBJECTIVE: Even though they are identified on the basis of their induction by elevated temperatures, heat shock proteins are induced by various stimuli. Such inducers include viral infection, exposure to ethanol, sodium arsenite, steroid hormones and heavy metals. They become involved under pathological conditions, such as anoxia/ischemia, reperfusion injury and oxidative stress in the heart. In this research, the cellular damages and changes of Hsp25 and alphaB crytallin were investigated under oxidative stress induced H2O2 treatment in a cultured cardiomyoblast cell line, H9c2. MATERIALS AND METHODS: The morphological changes of the H9c2, and the distributions of Hsp25 and alphaB crytallin, were examined with phase-contrast, confocal and electron microscopy. The levels of Hsp25 and alphaB crytallin expressions within the cells were also checked. RESULTS: After exposure to a low concentration (10 micrometer) of H2O2, the amounts of Hsp25 and alphaB crytallin were increased more than in the control and heat shock groups. They were particularly localized in the perinuclear region and along the actin filament. When exposed to a high concentration (more than 100 micrometer) of H2O2, many cells developed apoptotic features, such as vacuolization, blebbing and apoptotic body formations. The amounts of Hsp25 and alphaB crytallin were decreased more than in the control group. However, a weak reaction in the perinuclear region and along the actin filaments still remained when observed under confocal microscopy. CONCLUSION: These results suggest that the elevation of the expressions of Hsp25 and alphaB crytallin were dependent on the concentration of H2O2. Additionally, the redistribution of these small Hsps is closely associated with the actin filaments, as well as the nuclei. Therefore, much more research will be essential for the elucidation of roles of Hsp25 and alphaB crytallin in oxidative stress, such as ischemia or ischemic-reperfusion injury.
Actin Cytoskeleton ; Actins* ; Blister ; Cell Line* ; Ethanol ; Heart ; Heat-Shock Proteins* ; Hot Temperature* ; Ischemia ; Metals, Heavy ; Microscopy, Confocal ; Microscopy, Electron ; Oxidative Stress ; Reperfusion Injury ; Shock ; Sodium

PURPOSE: The purpose of this study is to evaluate the expression of E-cadherin and cathepsin-B in prostatic carcinomas and correlate with the Gleason grades. MATERIALS AND METHODS: The expressions of E-cadherin and cathepsin B were examined by the immunohistochemical technic using the antibodies against the E-cadherin and cathepsin B on the paraffin block sections of 56 prostatic carcinomas with evaluation of Gleason grading. RESULTS: E-cadherin expression in normal epithelium was membranous intercellular expression and those of prostate carcinomas were aberrant expressions such as negative expression or cytoplasmic presentation. The expressivity of the E-cadherin according to the progression of the Gleason grading revealed negative membranous expression and tendency of gradual increase of aberrant expression. The normal prostate and BPH revealed expression of cathepsin B mostly in the basal layers of acini as cytoplasmic reaction and the stromal macrophages and microvessel wall also showed positive expression. The prostatic carcinoma showed cytoplasmic positivity in the cancer cells and the expression rate was increased from Gleason grade 2 to Gleason grade 4. But the Gleason grade 5 tissue revealed decreased or negative expression. The Gleason grade 4, especially in the invasive cells and invasive edges, revealed the most intense and frequent expression of cathepsin B and this findings were consistent with the nonnal function of the cathepsin B as a protease degrading the extracellular matrix proteins. CONCLUSION: E-cadherin expression was aberrant after Gleason grade 6 related with high histologic grades. It is suggested that the E-cadherin expression could tell the potential cancer progression as a tumor suppression factor. The cathepsin B was most strongly expressed in basal cells of the benign prostatic acini and the cancer nests of Gleason grade 4, which tells the possibility that cathepsin B could be a marker of basocellular differentiation and of assessing stromal invasion of prostatic carcinomas.
Antibodies ; Cadherins* ; Cathepsin B* ; Cathepsins* ; Cytoplasm ; Epithelium ; Extracellular Matrix Proteins ; Macrophages ; Microvessels ; Neoplasm Grading ; Paraffin ; Prostate

South Korea implemented interventions to curb the spread of the novel coronavirus disease 2019 (COVID-19) pandemic with discovery of the first case in early 2020. Mathematical modeling designed to reflect the dynamics of disease transmission has been shown to be an important tool for responding to COVID-19. This study aimed to review publications on the structure, method, and role of mathematical models focusing on COVID-19 transmission dynamics in Korea. In total, 42 papers published between August 7, 2020 and August 21, 2022 were studied and reviewed. This study highlights the construction and utilization of mathematical models to help craft strategies for predicting the course of an epidemic and evaluating the effectiveness of control strategies. Despite the limitations caused by a lack of available epidemiological and surveillance data, modeling studies could contribute to providing scientific evidence for policymaking by simulating various scenarios.

This study attempted to examine the nutrition knowledge of caregivers for the elderly and the diet-related medical treatment and dietary assistance given by them. Thus, this research is a descriptive study focusing on the current nutritional knowledge of caregivers and the dietary assistance given by them. The survey included a questionnaire (nutritional knowledge level of digestive system diseases, diabetes, cardiovascular risk, brain disease, bone disease, and meal assistance performance status) for caregivers working in nursing homes for the elderly. Self-administered questionnaires were completed by 235 caregivers between February and March 2020. The results were analyzed using the SPSS 25.0, and the significance test of each question was verified by the Friedman test and the Chi-square independence test. The number of elderly people who needed meal assistance from caregivers was 4.4 more than the average. The most common types of meal assistance were partial assistance (59.20%) and the task of when to stop eating for the elderly (58.71%). Besides, the higher nutritional knowledge level of the caregivers, the more the time spent on services related to meal assistance (P＜0.001), and the higher the meal assistance level. The disease state of the elderly was considered the most relevant (P＜0.001). Caregivers with high levels of knowledge and offering significant meal assistance were found to have received nutrition education (P＜0.001). Up to 80% of the respondents needed nutrition education, and most of them answered that they needed education on appropriate management methods for the specific disease state of the elderly (P＜0.01). Accordingly, providing nutrition education for caregivers for the elderly should be a means to improve their ability to offer meal assistance.