No abstract available.
Blood Transfusion* ; Lymphocytes*

No abstract available.
Animals ; Burns* ; Rats*

No abstract available.
Diagnosis* ; Evoked Potentials, Somatosensory* ; Trigeminal Nerve Injuries* ; Trigeminal Nerve*

No abstract available.
Fournier Gangrene* ; Myocutaneous Flap*

During the microvascular operation, vasospasm can be disastrous because it may cause necrosis of the flap. But we could not fully understand the pathophysiology of contraction of operated vessels. We performed microvascular anastomosis of the femoral artery in the rats and decided the maximum initia tension and intensity of electrical stimulus. At the site of operation, postoperative 1st, 2nd, 3rd, 5th and 7th days, we took the samples of vessels including microvascular anastomotic site. Using myometry, we observed the responses of the samples responding to the stimuli and compared the results to that of the other unoperated femoral artery. The contractibility of the artery was increased in the first day and remained continuously until the postoperative 7th days.
Animals ; Arteries ; Femoral Artery* ; Necrosis ; Rats*

In this article, a part of fund and grant supports was omitted unintentionally.

PURPOSE: Previously, we reported the presence of virus-encoded microRNAs (miRNAs) in the urine of prostate cancer (CaP) patients. In this study, we investigated the expression of two herpes virus-encoded miRNAs in prostate tissue. METHODS: A total of 175 tissue samples from noncancerous benign prostatic hyperplasia (BPH), 248 tissue samples from patients with CaP and BPH, and 50 samples from noncancerous surrounding tissues from these same patients were analyzed for the expression of two herpes virus-encoded miRNAs by real-time polymerase chain reaction (PCR) and immunocytochemistry using nanoparticles as molecular beacons. RESULTS: Real-time reverse transcription-PCR results revealed significantly higher expression of hsv1-miR-H18 and hsv2-miRH9- 5p in surrounding noncancerous and CaP tissues than that in BPH tissue (each comparison, P<0.001). Of note, these miRNA were expressed equivalently in the CaP tissues and surrounding noncancerous tissues. Moreover, immunocytochemistry clearly demonstrated a significant enrichment of both hsv1-miR-H18 and hsv2-miR-H9 beacon-labeled cells in CaP and surrounding noncancerous tissue compared to that in BPH tissue (each comparison, P<0.05 for hsv1-miR-H18 and hsv2- miR-H9). CONCLUSIONS: These results suggest that increased expression of hsv1-miR-H18 and hsv2-miR-H95p might be associated with tumorigenesis in the prostate. Further studies will be required to elucidate the role of these miRNAs with respect to CaP and herpes viral infections.
Carcinogenesis ; Herpesviridae ; Humans ; Hyperplasia* ; Immunohistochemistry ; MicroRNAs ; Nanoparticles ; Prostate* ; Prostatic Hyperplasia ; Prostatic Neoplasms ; Real-Time Polymerase Chain Reaction