1.Compare the effect of lipo- and normal prepared Sodium Morrhuate on ECV-304 cell line.
Jun-bo TU ; Hai-long LAN ; Zhuang-qun YANG ; Tie-liang ZHANG ; Yong SONG ; Zhe XING
West China Journal of Stomatology 2006;24(6):491-494
OBJECTIVETo compare the effect of Sodium Morrhuate on ECV-304 between its lipo- and normal preparation.
METHODSThe ECV-304 cell line was supplemented with Sodium Morrhuate and lipo-Sodium Morrhuate in order, and the result on morphology (microscope, Giemsa Staining and electron microscope), cell activity (MTT), and flow cytometer between the two preparation were compared.
RESULTSIn normal preparation group, cell's edema occurred. Chromatin was like catkins. Tumefaction and degeneration of mitochondrion and endoplasmic reticulum appeared. In lipo-Sodium Morrhuate group, the membrane was creased and processus appeared. Chromatin aggregates to the membrane of nucleus was like crescent, and then broken. The apoptotic body was formed. MTT changes showed that the curve of the normal preparation group was steep and the change time was short relatively, which cues the vital cells decreased sharply. The curve of lipo-Sodium Morrhuate group was gentle and the change time was long relatively, which cues the vital cells decreased slowly. The flow cytometer showed that typical apoptosis peak appeared.
CONCLUSIONThe normal preparation group shows an acute toxic effect on ECV-304 cell line, which result in a necrosis course, while lipo-Sodium Morrhuate shows a gradual releasing process, which may indicate a apoptosis course.
Animals ; Apoptosis ; Cell Line ; Humans ; Necrosis ; Sclerosing Solutions ; Sodium Morrhuate
2.The experimental study of lipo-sodium morrhuate promoting apoptosis of ECV-304 cells.
Zhuang-qun YANG ; Hai-long LAN ; Jun-bo TU ; Yong SONG ; Tie-liang ZHANG ; Zhe XING
Chinese Journal of Stomatology 2007;42(3):188-189
OBJECTIVETo evaluate the effect of lipo-sodium morrhuate on ECV-304 cell line.
METHODSThe effect lipo-sodium morrhuate was evaluated by toxicology trial (MTT), electron microscope, DNA electrophoresis and flow cytometer.
RESULTSThe toxicology results showed, that the number of vital cells in lipo-sodium morrhuate group decreased slowly. The electron microscope exhibited apoptosis in the lipo-sodium morrhuate group. And there were typical DNA ladder in DNA electrophoresis and typical apoptosis peak in flow cytometer. The apoptosis rate was 22.23%.
CONCLUSIONSUnlike the normal preparation of sodium morrhuate, lipo-sodium morrhuate could induce apoptosis of ECV-304 cell line.
Apoptosis ; drug effects ; Cell Proliferation ; drug effects ; Cells, Cultured ; Endothelial Cells ; pathology ; Humans ; Liposomes ; Sodium Morrhuate ; pharmacology ; Umbilical Veins ; cytology
3.Comparison of the clinical therapeutic effects between local injection of pingyangmycin and sodium morrhuate on oral and maxillofacial venous malformation.
Wei HE ; Ai-Ling GU ; Jun-Lan SHANG
Chinese Journal of Stomatology 2007;42(5):308-309
OBJECTIVETo observe and compare the curative effect of pingyangmycin and sodium morrhuae respectively in the treatment of oral and maxillofacial venous malformation.
METHODSForty cases of oral and maxillofacial venous malformation were induced in this study, 20 of which were given local injection of low dose of pingyangmycin, and 20 given local injection of 5% sodium morrheate. The therapeutic effects were observed.
RESULTSThe effective cases of pingyangmycin was 19, and the effective cases of sodium morrhuate was 12. The side effect was found in 10 cases of, and in 15 cases sodium morrhuate group, the former was mainly pingymangmycin group systemic, the later was mainly local. The difference was significant between the two groups (P < 0.05).
CONCLUSIONSPingyangmycin is more effective in effective rate and side effect treatment of venous hemangioma than sodium morrhuate.
Adolescent ; Adult ; Bleomycin ; administration & dosage ; analogs & derivatives ; therapeutic use ; Child ; Child, Preschool ; Female ; Follow-Up Studies ; Humans ; Infant ; Injections, Intralesional ; Male ; Middle Aged ; Sclerosing Solutions ; therapeutic use ; Sodium Morrhuate ; administration & dosage ; therapeutic use ; Treatment Outcome ; Vascular Malformations ; therapy ; Young Adult
4.A Study on the Laboratory Findings in Experimental Osteomyelitis Caused by Staphylococcus aureus
Chung Soo HWANG ; Moon Sang CHUNG ; Moon Sik HAHN ; Ho Sung SONG ; Min Young CHUNG
The Journal of the Korean Orthopaedic Association 1981;16(4):755-770
Even though various kinds of diagnostic and therapeutic measures have been used for the detection and cure of the acute osteomyelitis, this disease is difficult to be eradicated in many clinical cases. However it is rather easy to treat once this disease can be detected in early stage. So far radiological examination and many kinds of maneuvers including blood culture, clinical laboratory, xeroradiography, computed tomography have been performed for the early detection of this disease but invalid in the most cases. Recently some authors reported that the bone scanning was useful for the early detection of acute osteomyelitis, but this method also had difficulty in differential diagnosis and uncertaninty of its activity. This study aims to try to find methods of early detection of acute osteomyelitis. Experimental osteomyelitis was developed by the inoculation of Staphylococcus aureus to proximal tibias of 39 young rabbits already sclerosed by solution of sodium morrhuate. By comparing the clinical, radiological, bacteriological and laboratory findings in sequence from 2 days to 7 weeks after inoculation, the following results were obtained. 1. The incidence of experimental osteomyelitis after inoculation of staphylococcus aureus SNUH-S-188 in suspension of 1×10(7) colony forming units was 84%, 74%, and 79% in radiological, blood culture and pathologic examination respectively. 2. The leucocytosis was noticed only in early stage and slight degree of anemia was found during the 1st and 2nd weeks after inoculation. The sedimentation rate was increased only in early acute stage and same was eosinophil count. The leucocyte alkaline phosphatase(LAP) and platelet count. showed no significant abnormal changes. 3. Lymphocyte and eosinophil turned over from normal and eosinophil was predominant in experimental osteomyelitis. 4. Serum calcium, phosphorus, total protein and albumin showed significant decrease in all stages and was conversely paralle1 to changes in groin temperature. The level of BUN was normal indicating normal kidney function in experimental osteomyelitis. 5. Even though the enzymes of which showed no significant changes enrolled alkaline phosphatase and serumgluiamicoxalic-transaminase(S-GOT), but some increa.e was attained to 27% and 44% respectively. 6. It is important and significant to find continuous increase of lactic dehydrogenase (LDH) and creatine phosphokinase (CPK) since 48 hours after inoculation and author, think these will be the reliable and sensitive indices in early detection of the development of osteomyelitis and efficacy of therapeutic measures.
Alkaline Phosphatase
;
Anemia
;
Calcium
;
Creatine Kinase
;
Diagnosis, Differential
;
Eosinophils
;
Groin
;
Incidence
;
Kidney
;
Lymphocytes
;
Methods
;
Osteomyelitis
;
Oxidoreductases
;
Phosphorus
;
Platelet Count
;
Rabbits
;
Sodium Morrhuate
;
Staphylococcus aureus
;
Staphylococcus
;
Stem Cells
;
Tibia
;
Xeroradiography