BACKGROUND: The pathogenesis of psoriasis may involve the interleukin (IL)-23 and Th17-mediated immune responses. Th17 cells secret IL-17 and IL-22, which mediates dermal inflammation and acanthosis. OBJECTIVE: As inhibitor of nuclear factor kappaB kinase-alpha (IKKalpha) has been previously identified as a primary regulator of keratinocyte differentiation and proliferation, we proposed that IL-17 and IL-22 might affect keratinocyte differentiation by changing the expression of IKKalpha. METHODS: We employed HaCaT cells maintained culture medium at a low calcium concentration (0.06 mM) and induced differentiation by switching to the high concentration (2.8 mM) media with IL-17 or IL-22, then compared the IKKalpha expression and the cell cycle. We employed reconstituted human epidermal skin (Neoderm) and mice ears for the in vivo studies. RESULTS: Elevated calcium concentration induced IKKalpha expression and terminal differentiation with cell cycle arrest in HaCaT cell cultures. Moreover, IL-17 and IL-22 treatment also induced IKKalpha in HaCaT cells and reconstituted human epidermis. IKKalpha induction was also noted, following the injection of IL-17 and IL-22 into mice ears. CONCLUSION: Although the induction of IKKalpha was accompanied by keratinocyte differentiation, IL-17 and IL-22 did not affect calcium-mediated differentiation or the cell cycle. Rather, IL-17 and IL-22 appear to contribute to the inflammation occurring via the induction of IKKalpha from keratinocytes or skin layers.
Animals ; Calcium ; Cell Culture Techniques ; Cell Cycle ; Cell Cycle Checkpoints ; Cell Differentiation ; Ear ; Epidermis ; Humans ; I-kappa B Kinase ; Inflammation ; Interleukin-17 ; Interleukins ; Keratinocytes ; Mice ; Psoriasis ; Skin ; Th17 Cells

Nasal cavity foreign bodies are common accidents in children, especially between the newly born and four years of age, and sometimes lead to complications such as epistaxis, vestibulitis, sinusitis bronchoaspiration and nasal septal damage etc. Diagnosis is often made with anterior rhinoscopy, but sometimes nasal fibroendoscopy and imaging may be useful. If the diagnosis is confirmed, prompt foreign body removal should be done to avoid complications. We have recently experienced a case of nasal septal perforation secondary to magnetic nasal foreign bodies attracting each other across the nasal septum. After the removal of foreign bodies, the nasal septal perforation was repaired with acellular human dermal allograft.
Child ; Collagen ; Epistaxis ; Foreign Bodies ; Humans ; Magnetics ; Magnets ; Nasal Cavity ; Nasal Septal Perforation ; Nasal Septum ; Sinusitis ; Transplantation, Homologous ; Transplants

Laryngomalacia is the most common congenital laryngeal anomaly. Usually, the symptoms of laryngomalacis are mild and the disease does not interfere with the general body growth as it resolves itself spontaneously. Occasionally, however, it causes a failure to grow, apnea or cardiopulmonary diseases. We have recently experienced a case of severe laryngomalacia neonate. Aryepiglottoplasty with apnea technique was carried out under the general anesthesia using CO2 Laser.
Anesthesia, General ; Apnea ; Congenital Abnormalities ; Humans ; Infant, Newborn ; Laryngomalacia ; Lasers, Gas

Backgroud and Objectives: 18F-FDG Positron emission tomography (18F-FDG PET) is a noninvasive diagnostic tool for many kinds of human cancer, where glucose transporter-1 (Glut-1) acts as a main transporter in the uptake of 18F-FDG in cancer cells. The object of this study is to assess the expression of Glut-1 in human papillary carcinoma and the relationship between the expression and the uptake of 18F-FDG PET. SUBJECTS AND METHOD: We evaluated 30 patients diagnosed as papillary carcinoma. Tumor sizes were measured and Glut-1 expression rate (ER), expression intensity (EI) and total expression score (ES) were analyzed. 18F-FDG PET was performed in 19 patients and standardized uptake value (SUV) was measured in each case. The correlations between ER and SUV, ES and SUV, tumor sizes and SUV, ER and tumor sizes were analyzed statistically. RESULTS: 96.7% (29/30) of tumors were Glut-1 positive, the mean ER was 67.42+/-22.89% and the mean ES 131.8+/-71. Tumor cells showed higher expression of Glut-1 than normal thyroid tissue. 18F-FDG uptake was positive in 81% (17/21) of solitary thyroid papillary carcinoma and negative in 19% (4/21). The average SUV of the PET positive group was 6.75+/-4.8, ER 71.25 +/-20.6% and ES 134.68+/-51.4. The average size of PET positive tumors was 3.37+/-2.94 cm2 and that of negative tumors was 0.43+/-0.45 cm2. Both ER and ES of Glut-1 were correlated with SUV significantly. The size and SUV were also correlated significantly. But the size and ER were not correlated significantly. CONCLUSION: Thyroid papillary carcinoma has high ER of Glut-1 and there is a positive correlation between Glut-1 expression and the uptake of 18F-FDG PET. The size of tumor can also affect the 18F-FDG uptake. But there is no correlation between the size and Glut-1 expression and further studies are needed to find the mechanisms and to decide the cut-off value.
Carcinoma, Papillary ; Electrons ; Fluorodeoxyglucose F18 ; Glucose ; Humans ; Positron-Emission Tomography ; Thyroid Gland

OBJECTIVES: Recent genetic studies have suggested a preferential transmission of the Dopamine D5 receptor gene (DRD5) 148bp marker allele. The aim of this study is to test the association between DRD5 and ADHD. METHODS: 106 Korean children with ADHD and their parents were analyzed using the transmission disequilibrium test (TDT) and haplotype-based haplotype relative risk test (HHRR). And also the ADHD children were compared with 212 age and gender matched normal controls. RESULTS: We found the evidence for an association of short alleles of DRD5 dinucleotide repeat polymorphism in both case control and family based studyies. Additionally, we observed some evidence for biased transmission of allele 152 bp and 144 bp. CONCLUSION: Our results lend credence to the notion that the relationship between ADHD and DRD5 is complex. The number of cases and informative transmissions in our study were small, therefore it would be premature to make any conclusions concerning the role of DRD5 in ADHD. Further work is needed to support these findings.
Alleles ; Attention Deficit Disorder with Hyperactivity* ; Bias (Epidemiology) ; Case-Control Studies* ; Child ; Dinucleotide Repeats ; Dopamine* ; Haplotypes ; Humans ; Parents ; Receptors, Dopamine D5*

OBJECTIVES: Recent genetic studies have suggested a preferential transmission of the Dopamine D5 receptor gene (DRD5) 148bp marker allele. The aim of this study is to test the association between DRD5 and ADHD. METHODS: 106 Korean children with ADHD and their parents were analyzed using the transmission disequilibrium test (TDT) and haplotype-based haplotype relative risk test (HHRR). And also the ADHD children were compared with 212 age and gender matched normal controls. RESULTS: We found the evidence for an association of short alleles of DRD5 dinucleotide repeat polymorphism in both case control and family based studyies. Additionally, we observed some evidence for biased transmission of allele 152 bp and 144 bp. CONCLUSION: Our results lend credence to the notion that the relationship between ADHD and DRD5 is complex. The number of cases and informative transmissions in our study were small, therefore it would be premature to make any conclusions concerning the role of DRD5 in ADHD. Further work is needed to support these findings.
Alleles ; Attention Deficit Disorder with Hyperactivity* ; Bias (Epidemiology) ; Case-Control Studies* ; Child ; Dinucleotide Repeats ; Dopamine* ; Haplotypes ; Humans ; Parents ; Receptors, Dopamine D5*