1.Chemical constituents of rhizomes of Smilax ferox.
China Journal of Chinese Materia Medica 2010;35(17):2293-2295
OBJECTIVETo study the chemical constituents of the rhizomes of Smilax ferox which was widely used in the folk.
METHODThe constituents of the rhizomes of S. ferox were isolated and purified by repeated silica gel column, MCI column, Sephadex LH-20 column and RP C18 column chromatography, and their structures were elueidated on the basis of spectral analysis.
RESULTSix compounds were identified as dihydrokaempferol (1), kaempferol (2), astilbin (3), engeletin (4), resveratrol (5), beta-sitosterol (6), respectively.
CONCLUSIONAll compounds were obtained from this plant for the first time.
Plant Extracts ; analysis ; isolation & purification ; Rhizome ; chemistry ; Smilax ; chemistry
2.Content detemination of total tannin in Smilax glabra with reddish brown and off-white colored cross section.
Hua ZHANG ; Li-Sha DONG ; Hu-Biao CHEN ; Xi-Cheng HE ; Xiang-Qian GE ; Xi-Gui ZHANG ; Ying-Ying ZHOU
China Journal of Chinese Materia Medica 2013;38(6):852-855
There are two types of decoction of Smilax glabra due to its reddish brown or off-white colored cross section. These two kinds of decoction were found that they have large difference in anti-inflammatory effects and chemical constituents in the preliminary experiments. Comparing and analyzing the content of total tannin in these two kinds of decoction of S. glabra from 28 areas by UV-Vis spectrophotometry were first used to provide some experimental and theoretical development and utilization of this medicinal resource and quality control. Also, the sample recovery test required in Chinese Pharmacopoeia was improved by adding tannic acid instead of gallic acid to samples.
Geography
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Pigmentation
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Reproducibility of Results
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Smilax
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chemistry
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Tannins
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analysis
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isolation & purification
3.Comparison of antioxidant, alpha-glucosidase inhibition and anti-inflammatory activities of the leaf and root extracts of Smilax china L..
Kyoung Kon KIM ; Yun Hwan KANG ; Dae Jung KIM ; Tae Woo KIM ; Myeon CHOE
Journal of Nutrition and Health 2013;46(4):315-323
This study was conducted in order to compare the biological activities of leaf and root water extracts of Smilax china L. (SC) by measuring the total polyphenol and flavonoid contents, anti-oxidant activity, inhibitory effect on alpha-glucosidase, and anti-inflammatory gene expression. The total polyphenol and flavonoid contents of SC leaf (SCLE) and root (SCRE) water extracts were 127.93 mg GAE/g and 39.50 mg GAE/g and 41.99 mg QE/g and 1.25 mg QE/g, respectively. The anti-oxidative activities of SCLE and SCRE were measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity assay and reducing power assay. Both SCLE and SCRE scavenged radicals in a concentration-dependent manner, and SCLE showed stronger radical scavenging activity and reducing power than SCRE; however, both SCLE and SCRE exhibited lower activities than ascorbic acid. Compared to the anti-diabetic drug acarbose, which was used as a positive control, SCLE and SCRE exhibited low alpha-glucosidase inhibition activities; nevertheless, the activity of SCLE was 3.7 fold higher than that of SCRE. Finally, SCLE caused significantly decreased expression of the LPS-induced cytokines, iNOS, and COX-2 mRNA in RAW264.7 cells, indicating anti-inflammatory activity. These results indicate that SCLE might be a potential candidate as an anti-oxidant, anti-diabetic, and anti-inflammatory agent.
Acarbose
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alpha-Glucosidases
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Ascorbic Acid
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Biphenyl Compounds
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China
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Cytokines
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Gene Expression
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Picrates
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RNA, Messenger
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Smilax
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Water
4.Phenylpropanoids and diphenylethene compounds from roots and rhizomes of Smilax scobinicaulis.
Ping WANG ; Jing XU ; Qi WANG ; Shi-Xiu FENG ; Tao CHEN ; Cun-Li ZHANG
China Journal of Chinese Materia Medica 2013;38(10):1531-1535
The chemical constituents were separated and purified from the roots and rhizomes of Smilax scobinicaulis by various chromatographic methods including silica gel, Sephadex LH-20. Their structures were obtained and identified as resveratrol-3-O-beta-D-glucopyranosyl-(1-->3)-beta-D-glucopyranoside (1), resveratrol (2), 8-viniferin (3), ethyl caffeate (4), 1-0-caffeoylglycerol (5), 1-O-p-coumaroylglycerol (6), 1-0-feruloylglycerol (7), grossamide (8), moracin M (9) on the analysis of spectroscopic data. Compound 1 was a new compound and compounds 3-5, 8,9 were separated from this plant for the first time.
Drugs, Chinese Herbal
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chemistry
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Mass Spectrometry
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Molecular Structure
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Plant Roots
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chemistry
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Rhizome
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chemistry
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Smilax
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chemistry
5.Study on separation and purification of total flavones from Smilax china by macroporous absorption resin.
Wen-xia LI ; Yan YAN ; Xiao-chuan YE ; Xiang-liang YANG
China Journal of Chinese Materia Medica 2007;32(13):1292-1295
OBJECTIVETo investigate the technological parameters of the purification process of total flavones from Smilax china with macroporous absorption resin.
METHODThe technical process for purification of total flavones with the optimum macroporous absorption resin was screened by yield of total flavones product.
RESULTThe D140 macroporous absorption resin had the best separating efficiency when the flavones content in the liquid was 0.5 g x mL(-1) equivalent to raw material, the volume of drug 18 BV (resin bad volume) with the adsorption-power 2 BV x h(-1), and the volume of 60% (mL x mL(-1)) ethanol as eluant 5-10 BV (resin bad volume) with desorption-power 1 BV x h(-1). The obtained flavones product has total flavones recovery rate of 84.72%.
CONCLUSIONThe treatment of regenerated resin is easy, this method is advisable.
Absorption ; Flavones ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Resins, Synthetic ; chemistry ; Rhizome ; chemistry ; Smilax ; chemistry ; Technology, Pharmaceutical ; methods
6.Optimization of purification conditions for with macroporous adsorption resin total saponins from smilax china.
Xiao-Shun SHU ; Zhong-Hong GAO ; Xiang-Liang YANG
China Journal of Chinese Materia Medica 2005;30(1):30-33
OBJECTIVETo develop a with high efficiency and practicality for separating and purifying total steroidal saponins lax china.
METHODUsing adsorption capacity and desorption rate of total steroidal saponins as the primary screening index, surveyed, and the optimized conditions of adsorption and desorption of total steroidal saponins were studied.
RESULTThe adsorption and desorption rate of total steroidal saponins reached 16 mg x mL(-1) and 90% respectively for macroporous resin HPD100 chosen. Macroporous resin HPD100 could be well used in separating and purifying total steroidal saponins from S. china.
Adsorption ; Anion Exchange Resins ; Hydrogen-Ion Concentration ; Plants, Medicinal ; chemistry ; Rhizome ; chemistry ; Saponins ; isolation & purification ; Smilax ; chemistry ; Spectrophotometry, Ultraviolet ; methods
7.Separation and purification of flavonoids from Smilax glabra by macroporous adsorption resin.
Shao-Wei HUANG ; Ru-An CHI ; Yue-Fei ZHANG ; Yuan-Xin WU ; Xia-Lian JU ; Jia GUO ; Qi-Mao HUANG
China Journal of Chinese Materia Medica 2008;33(10):1133-1138
OBJECTIVETo investigate the process of separating and purifying flavonoids from Smilax glabra.
METHODWith the yield of flavonoids as index, the optimum process of separating and purifying flavonoids from S. glabra Roxb was screened by static and dynamic adsorption tests.
RESULTThe static saturated adsorption capacity of D101 macroporous resin to flavonoids of S. glabra was 45.6 mg x g(-1) (dry resin). The optimum conditions of dynamic adsorption and elution were as that the pH, the concentration, the adsorption velocity of the extracting solution, and the adsorption capacity were 6.00 +/- 0.20, 4.2 mg x mL(-1), 2 mL x min(-1) and 15 mL, respectively. The adsorbed resin column was washed by 100 mL 60% ethanol with pH value of 8.00 +/- 0.20 at the eluting velocity of 3 mL x min(-1) after washed by 100 mL distilled water.
CONCLUSIONThe flavonoids of S. glabra was able to be easily separated and purified by D101 macroporous resin under the optimum conditions above, and the recovery rate was higher than 90%. The content of obtained flavonoids reached 62.6%, which was 2 times of the content before purification.
Adsorption ; Chromatography, Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; Flavonoids ; chemistry ; isolation & purification ; Resins, Synthetic ; chemistry ; Smilax ; chemistry
8.Spectrum-effect relationship between UPLC fingerprint of Smilax china and anti-pelvic inflammation in rats.
Si-Yi JIANG ; Xiao-Ying SONG ; Dan-Dan ZHANG ; Zheng-Lei LI ; Yu-Ying YANG ; Xin-Yao LUO ; Xiao-Chuan YE
China Journal of Chinese Materia Medica 2019;44(15):3323-3329
To study the correlation between ultra high performance liquid chromatography( UPLC) fingerprint of Smilax china and its anti-pelvic inflammatory effect,and to explore the pharmacodynamic material basis of S. china against pelvic inflammatory disease.UPLC fingerprints of 10 batches of S. china from different habitats were established,and the values of SOD,MDA,TNF-α,and IL-6 in rats with pelvic inflammation were measured. The weight of each single pharmacodynamics index to the total efficacy was determined by analytic hierarchy process,and the contribution of each peak in fingerprints to the each single pharmacodynamics index and total efficacy was analyzed by the grey relational analysis. Then the structures of chemical constituents at the identified peaks were confirmed by comparing with the reference substance. The 27 common characteristic peaks of UPLC fingerprints were all related to the anti-pelvic inflammation effect of S. china,of which 13 peaks were identified as peak 2( 3,5-dihydroxy-2-methylbenzoic acid-3-O-glucoside),peak 3( chlorogenic acid),peak 5( 2,7,4-trihydroxydihydroflavone-5-O-glucoside),peak 6( 7,4-dihydroxydihydroflavonol-5-O-glucoside),peak 7( taxifolin-7-O-glucoside),peak 9( taxifolin),peak 10( polydatin),peak 11( oxyresveratrol),peak 12( astilbin),peak15( resveratrol),peak 16( quercitrin),peak 18( engeletin) and peak 24( kaempferol). The correlation degree of 21 peaks and the total efficacy was greater than 0. 8,and the top 10 ranked by correlation degree were as follows: peak 1,3,7,19,18,17,4,11,16,and 21. The results showed that the anti-pelvic inflammation effect of S. china was achieved by the combined action of pharmacodynamic substances. In order to control the quality of S. china and its prepared slices more effectively,the index components of content detection should be selected reasonably.
Animals
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China
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Chromatography, High Pressure Liquid
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Female
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Pelvic Inflammatory Disease
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drug therapy
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Phytochemicals
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pharmacology
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Plant Extracts
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pharmacology
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Rats
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Smilax
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chemistry
9.Antiobesity effects of the water-soluble fraction of the ethanol extract of Smilax china L. leaf in 3T3-L1 adipocytes.
Yun Hwan KANG ; Kyoung Kon KIM ; Dae Jung KIM ; Myeon CHOE
Nutrition Research and Practice 2015;9(6):606-612
BACKGROUND/OBJECTIVES: Several medicinal properties of Smilax china L. have been studied including antioxidant, anti-inflammatory, and anti-cancer effects. However, the antiobesity activity and mechanism by which the water-soluble fraction of this plant mediates its effects are not clear. In the present study, we investigated the lipolytic actions of the water-soluble fraction of Smilax china L. leaf ethanol extract (wsSCLE) in 3T3-L1 adipocytes. MATERIALS/METHODS: The wsSCLE was identified by measuring the total polyphenol and flavonoid content. The wsSCLE was evaluated for its effects on cell viability, lipid accumulation, glycerol, and cyclic adenosine monophosphate (cAMP) contents. In addition, western blot analysis was used to evaluate the effects on protein kinase A (PKA), PKA substrates (PKAs), and hormone-sensitive lipase (HSL). For the lipid accumulation assay, 3T3-L1 adipocytes were treated with different doses of wsSCLE for 9 days starting 2 days post-confluence. In other cell experiments, mature 3T3-L1 adipocytes were treated for 24 h with wsSCLE. RESULTS: Results showed that treatment with wsSCLE at 0.05, 0.1, and 0.25 mg/mL had no effect on cell morphology and viability. Without evidence of toxicity, wsSCLE treatment decreased lipid accumulation compared with the untreated adipocyte controls as shown by the lower absorbance of Oil Red O stain. The wsSCLE significantly induced glycerol release and cAMP production in mature 3T3-L1 cells. Furthermore, protein levels of phosphorylated PKA, PKAs, and HSL significantly increased following wsSCLE treatment. CONCLUSION: These results demonstrate that the potential antiobesity activity of wsSCLE is at least in part due to the stimulation of cAMP-PKA-HSL signaling. In addition, the wsSCLE-stimulated lipolysis induced by the signaling is mediated via activation of the beta-adrenergic receptor.
3T3-L1 Cells
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Adenosine Monophosphate
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Adipocytes*
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Blotting, Western
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Cell Survival
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China*
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Cyclic AMP-Dependent Protein Kinases
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Ethanol*
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Glycerol
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Lipolysis
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Plants
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Smilax*
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Sterol Esterase
10.Study of the mechanisms underlying increased glucose absorption in Smilax china L. leaf extract-treated HepG2 cells.
Yun Hwan KANG ; Dae Jung KIM ; Kyoung Kon KIM ; Sung Mee LEE ; Myeon CHOE
Journal of Nutrition and Health 2014;47(3):167-175
PURPOSE: Previous studies have shown that treatment with Smilax china L. leaf extract (SCLE) produces antidiabetic effects due to alpha-glucosidase inhibition. In this study, we examined the mechanism underlying these antidiabetic effects by examining glucose uptake in HepG2 cells cultured with SCLE. METHODS: Glucose uptake and glucokinase activity were examined using an assay kit. Expression of glucose transporter (GLUT)-2, GLUT-4, and HNF-1alpha was measured by RT-PCR or western blot. RESULTS: Treatment with SCLE resulted in enhanced glucose uptake in HepG2 cells, and this effect was especially pronounced when cells were cultured in an insulin-free medium. SCLE induced an increase in expression of GLUT-2 but not GLUT-4. The increase in the levels of HNF-1alpha, a GLUT-2 transcription factor, in total protein extract and nuclear fraction suggest that the effects of SCLE may occur at the level of GLUT-2 transcription. In addition, by measuring the change in glucokinase activity following SCLE treatment, we confirmed that SCLE stimulates glucose utilization by direct activation of this enzyme. CONCLUSION: These results demonstrate that the potential antidiabetic activity of SCLE is due at least in part to stimulation of glucose uptake and an increase in glucokinase activity, and that SCLE-stimulated glucose uptake is mediated through enhancement of GLUT-2 expression by inducing expression of its transcription factor, HNF-1alpha.
Absorption*
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alpha-Glucosidases
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Blotting, Western
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China*
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Glucokinase
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Glucose Transport Proteins, Facilitative
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Glucose*
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Hep G2 Cells*
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Hepatocyte Nuclear Factor 1-alpha
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Smilax*
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Transcription Factors