The relevance analysis methods for the association between environmental factors (air pollutants, fine particles, climate conditions, toxic materials) and diseases (lung cancer and asthma, etc) were studied by mining the open access scientific data in American environmental health field, which realized their practical application and can thus provide reference for studying the association between environments and diseases in our country.

A model of classification techniques was established according to the sex and age by quantitative analy-sis of the risk factors for air popplution-induced respiratory diseases using the C4 .5 decision-making tree classifi-cation.The classification-based quantitative analysis was studied.The role of risk factors for respiratory diseases in 9 groups of people was assayed .This method can be used to analyze the risk factors for both respiratory diseases and other diseases in different populations, and can thus provide the evidence for their prevention and control.

Objective To explore the relationship of the expression, regulation and adhesion of PTA1 in pregnancy induced hypertension(PIH). Methods The expression,regulation and adhesion of PTA1 on vascular en- dothelial cells incubated by sera from 10 PIH patients and 10 normal pregnant women repectively was detected by flow cytometry. Results The positive rate of PTA1 15.51% (24h) and 6.32 % (48h) in PIH group is significantly higher than that 7.81% (24h) and 4.72 % (48h) in normal pregnant woman group( P < 0.01 ). After the incubation with platelets,the positive rate of PTA1 6.64% (24h) and 4.13% (48h) in PIH group is lower than before(P< 0.05). The PTA1 expression 8.11% (24h) and 4.28% (48h) in PIH group is much lower after blocking with PTA1/IgG than that before(P < 0.01 ). Conclusion There are some abnormal stimulated factors in PIH patients, which activate vascular endothelial cells. PTA1 plays an important role in the adhesion of platelets to vascular en- dothelial cells, PTA1 is closely related to the progress of PIH which indicates that PTA1 directly or indirectly takes part in the pathophysiologic consequences of PIH.

Objective To investigate the characteristics and differences of gastric flora in chronic non-atrophic gastritis and chronic atrophic gastritis associated with Helicobacter pylori(H.pylori).Methods A prospective study was adopted,and 24 patients with chronic gastritis caused by H.pylori infection were divided into chronic non-atrophic gastritis group(n=18)and chronic atrophic gastritis group(n=6).Characteristics of gastric mucosal flora in both groups were determined,and Alpha and Beta diversity analyses and species correlation analysis were performed.Results The gastric flora in both groups were mainly consisted of Proteobacteria,Bacteroidetes,Firmicutes,Actinobacteria,and Fusobacteria.Significant differences in species diversity and abundance were observed between the two groups(ANOSIM analysis,P=0.046).Fusobacterium(P=0.023),Leptotrichia(P=0.047),Prevotella(P=0.016),Sphingomonas(P=0.042),and Treponema(P=0.006)were significantly enriched in the chronic atrophic gastritis group,and H.pylori showed a decreasing trend(P=0.218).Some species of gastric mucosal flora showed negative correlations with H.pylori,and the number of species negatively associated with H.pylori in the gastric mucosal flora of chronic atrophic gastritis was significantly reduced.Conclusion In the gastric mucosa of chronic atrophic gastritis,the Fusobacterium,Leptotrichia,Prevotella,Sphingomonas,and Treponema are significantly enriched and associated with the progression of chronic gastritis.

Objective To investigate the characteristics and differences of gastric flora in chronic non-atrophic gastritis and chronic atrophic gastritis associated with Helicobacter pylori(H.pylori).Methods A prospective study was adopted,and 24 patients with chronic gastritis caused by H.pylori infection were divided into chronic non-atrophic gastritis group(n=18)and chronic atrophic gastritis group(n=6).Characteristics of gastric mucosal flora in both groups were determined,and Alpha and Beta diversity analyses and species correlation analysis were performed.Results The gastric flora in both groups were mainly consisted of Proteobacteria,Bacteroidetes,Firmicutes,Actinobacteria,and Fusobacteria.Significant differences in species diversity and abundance were observed between the two groups(ANOSIM analysis,P=0.046).Fusobacterium(P=0.023),Leptotrichia(P=0.047),Prevotella(P=0.016),Sphingomonas(P=0.042),and Treponema(P=0.006)were significantly enriched in the chronic atrophic gastritis group,and H.pylori showed a decreasing trend(P=0.218).Some species of gastric mucosal flora showed negative correlations with H.pylori,and the number of species negatively associated with H.pylori in the gastric mucosal flora of chronic atrophic gastritis was significantly reduced.Conclusion In the gastric mucosa of chronic atrophic gastritis,the Fusobacterium,Leptotrichia,Prevotella,Sphingomonas,and Treponema are significantly enriched and associated with the progression of chronic gastritis.

Toxocara vitulorum has been rarely reported in yaks at high altitudes and remote areas of Sichuan Province of Tibetan Plateau of China. The current study was designed to investigate the prevalence, associated risk factors, and phylogenetic characteristics of T. vitulorum in yak calves on the Qinghai Tibetan plateau. Fecal samples were collected from 891 yak calves and were examined for the presence of T. vitulorum eggs by the McMaster technique. A multivariable logistic regression model was employed to explore variables potentially associated with exposure to T. vitulorum infection. T. vitulorum specimens were collected from the feces of yaks in Hongyuan of Sichuan Province, China. DNA was extracted from ascaris. After PCR amplification, the sequencing of ND1 gene was carried out and phylogenetic analyses was performed by MEGA 6.0 software. The results showed that 64 (20.1%; 95% CI 15.8–24.9%), 75 (17.2; 13.8–21.1), 29 (40.9; 29.3–53.2), and 5 (7.6; 2.5–16.8) yak calves were detected out to excrete T. vitulorum eggs in yak calve feces in Qinghai, Tibet, Sichuan, and Gansu, respectively. The present study revealed that high infection and mortality by T. vitulorum is wildly spread on the Qinghai Tibetan plateau, China by fecal examination. Geographical origin, ages, and fecal consistencies are the risk factors associated with T. vitulorum prevalence by logistic regression analysis. Molecular detection and phylogenetic analysis of ND1 gene of T. vitulorum indicated that T. vitulorum in the yak calves on the Qinghai Tibetan plateau are homologous to preveiously studies reported.
Altitude ; Animals ; Ascaris ; Cattle* ; China* ; DNA ; Eggs ; Feces ; Logistic Models ; Mortality ; Ovum ; Polymerase Chain Reaction ; Prevalence* ; Risk Factors* ; Tibet ; Toxocara*

In order to understand the potential target and related mechanism of action of Termina-lia Chebula treatment,network pharmacology and molecular docking methods were used in this experiment,and the challenge test of Salmonella from yak was performed.The active ingredients and potential targets of Terminalia Chebula were screened through HERB cluster identification database,TCMSP database and SwissTargetPrediction web page tool,and"gastroenteritis"was searched through OMIM and GeneCards database.Cytoscape and STRING databases were used to construct the Terminalia Chebula PPI network to screen out key targets,the intersection targets between Terminalia Chebula and enteritis were obtained through Venny platform,and gene ontol-ogy(GO)and Kyoto encyclopedia database of genes and genomics(KEGG)were enriched through DAVID database.The core target of screening was verified by molecular docking.After that,the gastrointestinal inflammation model of mice was established,the pathological changes of gastroin-testinal tract were observed,and the effect of Terminalia Chebula on the target protein was veri-fied by Western blot test.The results showed that:after analyzing and sorting out 8 main active in-gredients of Terminalia Chebula,118 targets of Terminalia Chebula were screened,11 161 targets of gastroenteritis and 100 targets of intersection were obtained;the core targets of PTGS2,CASP3,SLC3A2,Bax,Bcl-2 and TP53 of Terminalia Chebula and enteritis were obtained through PPI network.GO and KEGG enrichment analysis collected 337 items and 138 items,respectively,mainly related to chemokine pathway,PI3K-Akt signaling pathway,apoptosis related pathway,i-ron ion transport related pathway,NF-κB signaling pathway,etc.The results of molecular docking showed that chebulidic acid,the first active component of chebulidic acid,can bind to Bax,Bcl-2,PTGS2 and SLC3A2 through hydrogen bonding,hydrophobic action,π-π packing force and other intermolecular forces.The pathological tissue sections showed that Terminalia Chebula could sig-nificantly recover gastrointestinal tissue injury.Western blot test results showed that Terminalia Chebula can regulate the process of apoptosis and iron death through Bax/Bcl-2 and PTGS2/SLC3A2 pathways to achieve the effect of treating intestinal inflammatory damage.The results showed that Terminalia Chebula can regulate the occurrence and development of enteritis by regu-lating apoptosis and iron death through Bax/Bcl-2 and PTGS2/SLC3A2 pathways.Terminalia Chebula has the characteristics of multi-target and multi-pathway in the treatment of enteritis.