1.Malignant intraductal papillary lesions of the breast: clinicopathological analyses of 28 cases
Siying FU ; Zhongsheng KUANG ; Mingneng REN ; Shanshan XIAO ; Yuhui XIE
Chinese Journal of Clinical and Experimental Pathology 2017;33(1):55-58
Purpose To discuss the clinical,histopathological characteristics,diagnosis,differential diagnosis and prognosis of malignant intraductal papillary lesions of the breast.Methods 28 cases of malignant intraductal papillary lesions of the breast were analyzed by histology and immunobistochemistry.Clinical and follow-up information was obtained.The published relevant literatures were reviewed.Results All the patients were females with a mean age of 55.7 years.The clinical features were a palpable mass or nipple discharge.28 cases were diagnosed including 22 cases of intraductal papaillary carcinoma,2 cases of encapsulated papillary carcinoma and 4 cases of solid papillary carcinoma.Microscopically,the tumor showed solid and papillary area inside the capsule wall with fine delicate fibrovascular septa.The tumor cells usually displayed low-grade nuclear features.Immunohistochemistry,the tumor cells revealed diffusely strong positive ER and PR in almost all cases and HER-2,CK5/6 were negative positive.All cases were negatiive for CK5/6,p63 and SMA in the celluar nodules.CD56,Syn and CgA were found positively in some solid papillary carcinoma cases.The average positive rate of Ki-67 in tumor cells was 5.3 %.27 patients were available for follow-up examination from 10 to 79 months and all the patients were alive.Conclusion Malignant intraductal papillary lesions of the breast most occurs in postmenopausal women.The diagnosis should be based on the clinical information,histopathological features and immunohistoehemistry stain due to its diverse histology.The main differential diagnosis is intraductal papilloma.This kind of lesion is a low grade malignant tumor with favorable prognosis.
2.Effects of Combination of Baoxinkang and Conventional Medicine Intervention on Cardiac Function and Adenylate Metabolism of Heart Failure Rats
Yongdun HONG ; Yifei SU ; Ziqing YE ; Jing ZHAO ; Xiaobing LI ; Siying FU
Journal of Guangzhou University of Traditional Chinese Medicine 2015;(1):97-102
Objective To evaluate the synergistic effect of Baoxinkang on chronic heart failure ( CHF) rats by observing the effect of the combination of Baoxinkang and conventional medicine intervention on cardiac function and adenylate metabolism. Methods Sixty Sprague-Dawley rats were randomly divided into 6 groups, namely sham-operation group, model group, Baoxinkang group ( Baoxinkang 1 020 mg/kg) , conventional medicine intervention group (metoprolol 10 mg/kg, captopril 5 mg/kg, and digoxin 0.022 5 mg/kg), combination group 1 ( conventional medicine intervention + Baoxinkang 1 020 mg/kg) , and combination group 2 ( conventional medicine intervention + trimetazidine 10 mg/kg) . Abdominal aora was constricted to establish CHF rat model. The rats except for the sham-operation group and model group were given the corresponding medicine according to the experimental design for 6 weeks. Echocardiography ( ECHO) was performed to evaluate the cardiac function of rats. High performance liquid chromatography was utilized to investigate the contents of myocardiac adenylate of adenosine triphosphate ( ATP) , adenosine diphosphate ( ADP) , adenosine monophosphate (AMP) . Total adenylate nucleotide pool (TAN) was equal to ATP+ADP+AMP, energy charge (EC) was equal to ( ATP+0.5 ×ADP) /TAN. Results The results of ECHO showed that the heart size was reduced, left ejection fraction and cardiac output were increased in the combination group 1 compared with the model group and conventional medicine intervention group. The levels of ATP, TAN, and EC were significantly increased, whereas the levels of ADP and AMP were decreased in the combination group 1 (P<0.05 or P<0.01). However, there were no significant differences of heart size, left ejection fraction, cardiac output, ATP, ADP, AMP, TAN or EC between combination group 1 and combination group 2 ( P>0.05) . Conclusion Baoxinkang may have some synergistic effect on the improvement of CHF rat cardiac energy metabolism disorder treated by conventional medicine.
4.Key points for establishing a whole-process clinical research management system
Wei LV ; Shuai FU ; Jiandong YU ; Siying NIU ; Baoshi YUAN ; Xia MENG
Chinese Journal of Medical Science Research Management 2021;34(2):117-120
Objective:To explore the key points and implementation of establishing a whole-process clinical research management system.Methods:Based on the problems in practice, combined with project management experiences, this article analyzed the construction of the whole-process clinical research management system.Results:The establishment of the management system provides a comprehensive and sustainable safeguard for clinical research, as well as the improvement of efficiency and quality of clinical research.Conclusions:The establishment of an effective whole-process management system for clinical research project is a useful exploration of the research service model in China.
5.Epidemiological study of high risk human papillomavirus infection in 25 to 54 years old married women in Beijing
Minghui WU ; Songwen ZHANG ; Weiyuan ZHANG ; Baoli ZHOU ; Zheng XIE ; Jiandong WANG ; Jing FENG ; Junhua WANG ; Jiwei JIANG ; Li ZHU ; Shiquan HUANG ; Jing PAN ; Xinzhi LIU ; Yunping ZHANG ; Wen ZHAO ; Hong LI ; Xiaohang LUO ; Kunchong SHI ; Guifeng WANG ; Liping FU ; Guixiang LI ; Hunfen TAO ; Chunxiang BAI ; Ruixia HE ; Lei JIN ; Guangmei LIU ; Kuixiang WANG ; Jialin YE ; Siying LIU ; Mei WANG ; Xueming YAN ; Guiling HU ; Rujing LIN ; Changyue SUN ; Hong ZENG ; Lirong WU ; Yali CHENG
Chinese Journal of Obstetrics and Gynecology 2009;44(12):892-897
Objective To investigate high risk human papillomavirus(HR-HPV)prevalence among married women in Beijing and to study the high risk flactors.nethods During March 2007 to September 2008.a total of 6185 married women sampled from 137 communities in 12 districts were screened bv HR-HPV DNA test and cytogical test.The interview was carried out with unified questionnaires.The databage was set up and twice entered in EpiDam 3.0.After checked up,the data were analyzed in SPSS 15.0.Results (1) The HR-HPV infection rate was 9.89%.The HR-HPV infection rate of the city zone,the suburb and the exurb were 9.34%,10.51% and 9.51% (P>0.05).The HR-HPV infection rate of the native and the oudander were 9.53%,11.30% (P<0.05).(2) The age distribution of HR-HPV infection was that the rate was around 10% among 25 to 44 age groups,which was the highest(11.21%) in 30 to 34 age group;then the rate was descended as the age raising,the rate of 50 to 54 age group was the lowest(7.78%).(3) Multiple logistic regression showed that the related risk factors of HR-HPV infection mainly included 1000 RMB and above of family income per person per month.possessing more than 1 sexual partner of her husband,outlander and hish levels of education.(4) The prevalence of cervical intraepithelial neoplasia(CIN)in HR-HPV positive group wag significantly higher than that in HR-HPV negative group(29.76% vs 3.32%,P<0.01).Conclusions(1)The HR-HPV infection rate among aged 25 to 54 years was 9.9% and there was no significant difference in area distribution.(2)The hish risk population which should strengthen screening was the married bearing-age women with high level of family income,outlander,high levels of education and her husband possessing more than 1 sexual partner.(3)HR-HPV infection is the main risk factor for CIN and cervical cancer.while does not provide a causal relationship with them.The high risk population should be checked regularly to understand the development of HR-HPV infection and CIN incidence.
6.A small molecule UPR modulator for diabetes identified by high throughput screening.
Valeria MARROCCO ; Tuan TRAN ; Siying ZHU ; Seung Hyuk CHOI ; Ana M GAMO ; Sijia LI ; Qiangwei FU ; Marta Diez CUNADO ; Jason ROLAND ; Mitch HULL ; Van NGUYEN-TRAN ; Sean JOSEPH ; Arnab K CHATTERJEE ; Nikki ROGERS ; Matthew S TREMBLAY ; Weijun SHEN
Acta Pharmaceutica Sinica B 2021;11(12):3983-3993
Unfolded protein response (UPR) is a stress response that is specific to the endoplasmic reticulum (ER). UPR is activated upon accumulation of unfolded (or misfolded) proteins in the ER's lumen to restore protein folding capacity by increasing the synthesis of chaperones. In addition, UPR also enhances degradation of unfolded proteins and reduces global protein synthesis to alleviate additional accumulation of unfolded proteins in the ER. Herein, we describe a cell-based ultra-high throughput screening (uHTS) campaign that identifies a small molecule that can modulate UPR and ER stress in cellular and