This article gives a brief account of the origin and development of pestle needle therapy, and its special acupoints, needling instruments and commonly used techniques, and explores the close relationship between pestle needle therapy and the traditional Chinese medical theory to seek more theory support of this therapy from traditional Chinese medicine. The article also summarizes clinical indications for pestle needle therapy and regularities in the practical application of this therapy to promote the inheritance and development of pestle needle therapy.

0.05). It shows that mushroom broth culture, which is processed conveniently and cheaply, has good practical value.

Aim To establish a mouse breast cancer model stab-ly expressing HER2. Methods 4T1-Luc mouse breast cancer cell line was transfected with the full-length human HER2 gene and selected with G418. The HER2 expression in 4T1-Luc stable cells was detected by fluorescence-activated cell sorting ( FACS) and Western blot. 4T1-Luc/HER2 cells were implanted into the mammary fat pads of BALB/c or nude mice. After tumor stabili-zation, mice were randomly assigned into 4 groups for treatment with PBS control, chA21, Trastuzumab, or chA21 plus Trastu-zumab. Tumor volumes were measured and tumor growth inhibi-tion ratios were calculated twice a week. At the end of experi-ment, tumor metastasis in mice was detected by bioluminescence imaging technology. Results Several 4T1-Luc/HER2 stable cell clones were obtained after G418 selection. FACS and West-ern blot analysis showed that all clones expressed HER2 protein at high levels. These 4T1-Luc/HER2 clones showed good tumor-igenicity in mice with steady tumor growth after one week of cell implantation. After 2-3 weeks, metastatic tumor cells were seen in the lung, cheek and groin areas. In BALB/c mice, the tumor growth inhibition ratio was 43. 3% in chA21 plus Trastuzumab group (P<0. 05 vs PBS control), which was higher than chA21 group (11. 1%) or Trastuzumab group (23%). In addition, the luminescence number and density of tumor metastases in lungs were significantly reduced in the antibody combination group. Conclusions The mouse model of spontaneously metastasizing breast cancer with HER2 overexpression is successfully estab-lished. The preliminary study suggests that anti-HER2 antibody combination of chA21 and Trastuzumab has excellent inhibitory effects on tumor growth and metastasis.

Chylous fistula is a postoperative complication following pancreatic surgery,it is mainly diagnosed according to the nature of the patient's drainage fluid (≥3 days after surgery,triglyceride concentration ≥ 1.2 mmol/L).The mechanism of occurrence includes obstruction,injury and exudation of the lymph-vessels.Risk factors can be concluded into 3 aspects:clinicopathological features,surgical procedure and postoperative management.Most of the chylorrhea can be cured by conservative treatment like modified dietary measures and somatostatin,while severe cases still requires intervention and surgical treatment.This article reviewed the risk factors and treatment approaches of postoperative chylous fistula,and elucidated relative mechanisms,hoping to provide guidance in clinical prevention,diagnosis and treatment of chylous fistula.

Objective:To test whether the fear-avoidance beliefs (FAB) of nursing staff in a hospital in Shanghai have a mediating effect between neck pain and quality of life.Methods:The cross-sectional survey method was used to obtain the general information, neck pain, FAB and quality of life scores of the subjects. The mediating effect analysis was used to verify potential mediating effect of FAB on neck pain and quality of life.Results:A total of 103 valid questionnaires were collected, 60 (58.25%,60/103) in the neck pain group and 43 (41.75%, 43/103) in the pain-free group. The independent sample T test found that the FABQ score was higher in the pain group than in the pain-free group ( P<0.05). The quality of life score was lower in the pain-free group, and the between-group difference of physical component summary (PCS) was statistically significant ( P < 0.05). The results of pearson correlation analysis showed a negative correlation between FABQ and quality of life scores ( rFABQ1-PCS=-0.32, rFABQ2-PCS=-0.34, rFABQ1-MCS=-0.20, rFABQ2-MCS=-0.32, P<0.05). Both Physical Activity Fear Avoidance Belief (FABQ-1) and Work Fear Avoidance Belief (FABQ-2) have a complete mediating effect between neck pain and PCS. The mediating effect magnitude of FABQ-1 was 0.575, and the mediating effect of FABQ-2 was 0.552. The mediating effect magnitude of FABQ-1 was enlarged and FABQ-2 was reduced after adding age as a covariate in the mediating effect analysis. The mediating effect of FABQ-1 was 0.601, and the mediating effect magnitude of FABQ-2 was 0.501. Conclusion:The fear avoidance belief of nursing staff in a hospital in Shanghai presents a complete mediating effect between neck pain and quality of life, and the mediating effect of Physical Activity Fear Avoidance Belief is stronger with age but the mediating effect of Work Fear Avoidance Belief is weaker.

Innovation is an important way to promote economic development and social progress. Recent years have seen rapid development of biological sciences. In response to social demands and the needs for developing an innovative country, fostering innovative talents in the field of biosciences has become a significant initiative supported by national policies and the needs from talent market. Taking the innovative talent training mode implemented by Zhejiang Normal University in the field of biological sciences as an example, this paper comprehensively introduces several key aspects of the mode. This includes establishing a mentorship system as the foundation, carrying out curriculum reform through project competitions and practical platforms, and promoting synergy among industry, academia, and research in talent training. This training mode has achieved positive results in practice, promoting the training of outstanding innovative talents in biological science majors, and may facilitate the reform of talent training in similar majors.
Humans ; Biological Science Disciplines ; Industry ; Policy ; Universities

Background Nursing staff are often exposed to a variety of occupational risk factors in the working environment, such as long working hours and heavy workload, which associated with adverse mental health outcomes. And these factors may not be randomly distributed across different levels. Objective To explore mental health risk factors of nursing staff by multilevel analysis. Methods A cross-sectional survey of nursing staff in Shanghai Municipality, Zhejiang Province, Guangxi Zhuang Autonomous Region, and Xinjiang Uygur Autonomous Region was conducted through convenience sampling from 2018 to 2021. Data were collected by self-report questionnaires. The mental component summaries of 12-Iitem Short Form Health Survey were used to evaluate the mental health status of nursing staff, and related factors were collected atindividual level, including gender, body mass index (BMI), smoking status, drinking status, working years, pain intensity of musculoskeletal disorders, and working hours per week, and at regional level, including gross domestic product (GDP) level of each province. A two-level model was established by incorporating both individual and regional factors, and deviance was used to test the goodness of fit of the model. A traditional generalized linear model was also established, and then compared with the multilevel model. Results A total of 567 nurses participated in this study, and the valid rate of questionnaire was 80.08%. The results of the multilevel model showed that the regional factor contributed 12.1% to the mental component summaries. As to the regional factor, GDP was negatively correlated with mental health of nursing staff, the adjusted OR (AOR) was −0.53 (95%CI: −0.66-−0.28). Among the factors at individual level, the mental component summaries of females were lower than those of males (AOR=−3.25, 95%CI: −4.73-−0.35); the longer the working years, the higher the mental health score (AOR=0.11, 95%CI: 0.06-0.20); working hours per week (AOR=−0.10, 95%CI: −0.14-−0.03) and pain intensity of musculoskeletal disorders (AOR=−0.05, 95%CI: −0.06-−0.03) were negatively correlated with mental component summaries. The results of the generalized linear model included the same factors as the multilevel model, but the 95%CIs of AOR of the factors in the multilevel model were narrower, and the deviation value of the multilevel model was the smallest, indicating that the goodness of fit of the multilevel model was better than that of the traditional linear model. Conclusion The mental health of nursing staff is not only affected by individual level factors, but also affected by regional level factors. It suggests that combining different levels of intervention measures can upscale the effect of improving mental health in nursing staff.

Humans ; Parkinson Disease/therapy* ; Phototherapy ; Sleep Wake Disorders

Recent advancement in natural language processing (NLP) and medical imaging empowers the wide applicability of deep learning models. These developments have increased not only data understanding, but also knowledge of state-of-the-art architectures and their real-world potentials. Medical imaging researchers have recognized the limitations of only targeting images, as well as the importance of integrating multimodal inputs into medical image analysis. The lack of comprehensive surveys of the current literature, however, impedes the progress of this domain. Existing research perspectives, as well as the architectures, tasks, datasets, and performance measures examined in the present literature, are reviewed in this work, and we also provide a brief description of possible future directions in the field, aiming to provide researchers and healthcare professionals with a detailed summary of existing academic research and to provide rational insights to facilitate future research.
Humans ; Natural Language Processing ; Surveys and Questionnaires