Objective:To construct the admission standard and job responsibility scheme for oncology nurse navigator (ONN) .Methods:Through literature analysis, semi-structured interview, group discussion and pre-consultation, the admission criteria and job responsibility scheme of ONN were preliminarily constructed. Using the purposive sampling method, a total of 22 experts from 16 hospitals in 11 provinces and cities including Sichuan, Xi'an, Chongqing, Wuhan, Shanghai, Hangzhou, Xiamen, Guangzhou, Tianjin, Beijing and Changsha were selected as the research objects from October to December 2021. Using the Delphi method, 22 experts were consulted for 2 rounds, and the AHP was used to determine the index weights.Results:The effective recovery rates of the two rounds of expert consultation were 91% (20/22) and 100% (20/20) , respectively. In the second round, the authority coefficient of experts was 0.875, and the Kendall's coordination coefficients of admission standard and job responsibilities were 0.164, 0.303 and 0.150, 0.203, 0.238, respectively (all P<0.05) . The final formed ONN admission standard and job responsibility scheme included 4 first-level indicators and 22 second-level indicators of the admission standard and 4 first-level indicators, 15 second-level indicators and 49 third-level indicators for job responsibilities. Conclusions:The ONN admission standard and job responsibility scheme determined by expert consultation is scientific, feasible, practical and innovative, which can provide a theoretical basis for the training and implementation of ONNs in China.

Objective:To construct the admission standard and job responsibility scheme for oncology nurse navigator (ONN) .Methods:Through literature analysis, semi-structured interview, group discussion and pre-consultation, the admission criteria and job responsibility scheme of ONN were preliminarily constructed. Using the purposive sampling method, a total of 22 experts from 16 hospitals in 11 provinces and cities including Sichuan, Xi'an, Chongqing, Wuhan, Shanghai, Hangzhou, Xiamen, Guangzhou, Tianjin, Beijing and Changsha were selected as the research objects from October to December 2021. Using the Delphi method, 22 experts were consulted for 2 rounds, and the AHP was used to determine the index weights.Results:The effective recovery rates of the two rounds of expert consultation were 91% (20/22) and 100% (20/20) , respectively. In the second round, the authority coefficient of experts was 0.875, and the Kendall's coordination coefficients of admission standard and job responsibilities were 0.164, 0.303 and 0.150, 0.203, 0.238, respectively (all P<0.05) . The final formed ONN admission standard and job responsibility scheme included 4 first-level indicators and 22 second-level indicators of the admission standard and 4 first-level indicators, 15 second-level indicators and 49 third-level indicators for job responsibilities. Conclusions:The ONN admission standard and job responsibility scheme determined by expert consultation is scientific, feasible, practical and innovative, which can provide a theoretical basis for the training and implementation of ONNs in China.

Uncovering the functionally essential variations related to tumorigenesis and tumor pro-gression from cancer genomics data is still challenging due to the genetic diversity among patients, and extensive inter-and intra-tumoral heterogeneity at different levels of gene expression regulation, including but not limited to the genomic, epigenomic, and transcriptional levels. To minimize the impact of germline genetic heterogeneities, in this study, we establish multiple primary cultures from the primary and recurrent tumors of a single patient with hepatocellular carcinoma (HCC). Multi-omics sequencing was performed for these cultures that encompass the diversity of tumor cells from the same patient. Variations in the genome sequence, epigenetic modification, and gene expression are used to infer the phylogenetic relationships of these cell cultures. We find the discrepancy among the relationships revealed by single nucleotide variations (SNVs) and transcriptional/epigenomic pro-files from the cell cultures. We fail to find overlap between sample-specific mutated genes and differ-entially expressed genes (DEGs), suggesting that most of the heterogeneous SNVs among tumor stages or lineages of the patient are functionally insignificant. Moreover, copy number alterations (CNAs) and DNA methylation variation within gene bodies, rather than promoters, are significantly correlated with gene expression variability among these cell cultures. Pathway analysis of CNA/DNA methylation-related genes indicates that a single cell clone from the recurrent tumor exhibits distinct cellular characteristics and tumorigenicity, and such an observation is further confirmed by cellular experiments both in vitro and in vivo. Our systematic analysis reveals that CNAs and epigenomic changes, rather than SNVs, are more likely to contribute to the phenotypic diversity among subpop-ulations in the tumor. These findings suggest that new therapeutic strategies targeting gene dosage and epigenetic modification should be considered in personalized cancer medicine. This culture model may be applied to the further identification of plausible determinants of cancer metastasis and relapse.

Objective By tracking survey of the mental disorder patients discharged from hospital , in order to propose continuum of care and social interventions strategy .Methods Using household survey method , totals of 525 elderly mental disorder patients from 2010 to 2012 were selected and surveyed using the Geriatric Depression Scale (GDS) and the Activities of Daily Living (ADL).Results Totals of 525 cases of senile mental disorders in community were surveyed with GDS .Totals of 86 patients scored 1 to 10 points, regarded as without depression, accounting for 40%.Totals of 79 patients scored 11 to 20 points, regarded as mild depression , accounting for 36.74%.Totals of 50 patients scored 21 to 30 points, regarded as severe depression , accounting for 23.25%.Totals of 422 patients (80.38%) had ADL score under 35 points, with different degree of lack of personal living capacity .Totals of 103 patients (19.62%) had ADL score between 40 to 100 points, with part or complete personal living capacity .Totals of 525 patients mainly depended on home care , within which 68.95%were taken care of by their family members and 11.43%were cared for by servants or hourly employee .Others (19.62%) were at nursing institution or hospitals taken care of by professional nurses .There was a big gap between the demand and supply of medical care service in community elderly long term care .Conclusions The problem of long-term care and social support of senile mental disorder patients is urgent , and based on the actual situation, we need to propose the corresponding social intervention strategies .

ObjectiveTo investigate the role and mechanism of action of Yinchenhao Decoction in inhibiting ferroptosis of hepatocytes in mice with autoimmune hepatitis. MethodsA total of 18 specific pathogen-free female C57BL/6 mice were selected and divided into normal group， model group， and treatment group using a random number table， with 6 mice in each group. The mice in the model group and the treatment group were injected with concanavalin A （Con A） via the caudal vein to establish a mouse model of autoimmune hepatitis， and those in the normal group were injected with normal saline. The mice in the treatment group were given prophylactic treatment with Yinchenhao Decoction （4.68 g crude drug/kg） by gavage at 14 days before modeling， and Con A was injected after the last gavage. The levels of alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， interferon gamma （IFN-γ）， tumor necrosis factor-α （TNF-α）， iron ion， glutathione （GSH）， reactive oxygen species （ROS）， adenosine triphosphate （ATP）， and malondialdehyde （MDA） were measured； liver index and spleen index were calculated； the expression levels of GPX4 and SLC7A11 were measured； liver histopathological changes were compared between groups. A one-way analysis of variance was used for comparison of normally distributed continuous data between three groups， and the least significant difference t-test was used for further comparison between two groups. ResultsCompared with the normal group， the model group had significant increases in liver index， spleen index， ALT， AST， IFN-γ， TNF-α， iron ion， ROS and MDA （all P<0.05） and significant reductions in the content of GSH and ATP and the protein expression levels of GPX4 and SLC7A11 （all P<0.05）. Compared with the model group， the treatment group had significant reductions in liver index， spleen index， ALT， AST， IFN-γ， TNF-α， iron ion， ROS and MDA （all P<0.05） and significant increases in the content of GSH and ATP and the protein expression levels of GPX4 and SLC7A11 （all P<0.05）. HE staining showed that compared with the normal group， the model group showed massive hepatocyte degeneration and necrosis and inflammatory cell aggregation at the portal area， and compared with the model group， the treatment group had alleviation of liver necrosis and inflammatory infiltration. ConclusionLiver injury induced by Con A may be associated with ferroptosis. Yinchenhao Decoction can increase the protein expression levels of SLC7A11 and GPX4 protein and thus inhibit ferroptosis of hepatocytes induced by Con A.