Objective To explore the effects of gait training with an alternative gait orthosis (AGO) on the neurogenic bladders of patients with spinal cord injury (SCI). Method Twelve patients with complete SCI at the thoracic and lumbar level were trained for 8 weeks on motor and bladder function. After that, specific gait training with an AGO was given for another 8 weeks. American Spinal Injury Association ( ASIA ) sensation and motor function assessments were done before and after the gait training with the AGO, and bladder capacity, residual urine and urine flow rates were also assessed. Results Compared with pre-treatment there was no significant difference in average ASIA senzsation and motor scores after training, but the maximum rate of urine flow, average urine volume and single urination had significantly increased and residual urine volume had significantly decreased. Conclusions Therapeutic gait training with an AGO can improve bladder function after SCI.

Objective To investigate the serum tumor necrosis factor like weak inducer of apoptosis(TWE AK),sterol regulatory element-binding protein 1(SREBP-1)levels in patients with prostate cancer(PC)and their relationship with clinical pathological characteristics and progression free survival prognosis.Method A total of 94 PC patients who underwent PC radical surgery in Wuhan Dongxihu District People's Hospital from January 2018 to January 2020 were selected as the PC group.Meanwhile,50 patients with benign prostatic hyperplasia(BPH)during the same period were selected as the BPH group,and 50 healthy individuals who underwent physical examination during the same period were selected as the control group.Enzyme linked immunosorbent assay(ELISA)was used to detect the expression levels of serum TWEAK and SREBP-1.Kaplan-Meier survival analysis was used to analyze the effects of serum TWEAK and SREBP-1 on the progression free survival in prostate cancer patients.Multivariate COX regression analysis was used to analyze factors affecting the prognosis of progression free survival in prostate cancer patients.Results The serum TWEAK(77.14±15.46 ng/L)and SREBP-1(334.14±33.81 ng/L)levels in the PC group were higher than those in the BPH group(38.69±10.58 ng/L,201.69±28.74 ng/L)and control group(36.26±10.27 ng/L,189.51±27.65 ng/L),with significant differences(t=23.752,25.249;34.636,37.821,all P＜0.05).There was a positive correlation between serum TWEAK and SREBP-1 expression in PC patients(r=0.668,P=0.001).The serum TWEAK and SREBP-1 levels in PC patients with Gleason score＞7,TNM stage Ⅲ,and preoperative prostate specific antigen(PSA)level ≥ 20 ng/ml were higher than those with Gleason score≤7,TNM stage Ⅰ～Ⅱ,and preoperative PSA level＜20ng/ml,with significance differences(t=8.465～16.597,all P＜0.05).The 3-year overall progression free survival rates of the TWEAK high expression and low expression groups were 60.42％(29/48)and 86.96％(40/46),respectively.The 3-year overall progression free survival rates of the SREBP-1 high expression and low expression groups were 57.78％(26/45)and 87.76％(43/49),respectively.The 3-year cumulative progression free survival rates of the TWEAK high expression group and the SREBP-1 high expression group were lower than those of the TWEAK low expression group and the SREBP-1 low expression group,and the differences were significant(Log rankx2=8.125,9.547,P=0.004,0.002).TNM stage Ⅲ(OR=1.448,P＜0.001),Gleason score＞7(OR=1.401,P＜0.001),preoperative PSA ≥ 20 ng/ml(OR=1.353,P＜0.001),serum TWEAK(OR=1.338,P＜0.001),and SREBP-1(OR=1.293,P＜0.001)were independent risk factors affecting the progression free survival prognosis of PC patients.Conclusion Serum TWEAK and SREBP-1 in prostate cancer patients were increased,and they were correlated with the clinical pathological characteristics of PC.They could be serum biomarkers for evaluating the prognosis of progression free survival.

Objective To investigate the relationships of preoperative serum V-set and immuno-globulin domain 4(VSIG4)and long chain non-coding ribonucleic acid(LncRNA)SBF2 antisense RNA1(SBF2-AS1)with acute kidneyinjury(AKI)after percutaneous nephrolithotomy in patients with renal calculus.Methods A total of 109 patients with renal calculus in the hospital from January 2020 to December 2022 were selected as research objects.Serum VSIG4 level and LncRNA SBF2-AS1 expression were detected in all the patients before operation,and incidence of AKI was recorded after operation.Multiple Logistic regression model was used to analyze the factors affecting AKI after percutaneous nephrolithotomy in patients with renal calculus;the receiver operating characteristic(ROC)curve was used to analyze the values of VSIG4 and LncRNA SBF2-AS1 in predicting AKI af-ter percutaneous nephrolithotomy in patients with renal calculus.Results In this study,16 cases had AKI after operation.The serum VSIG4 level in the AKI group was significantly lower than that in the non-AKI group,while the LncRNA SBF2-AS1 expression was significantly higher than that in the non-AKI group(P＜0.05).Multivariate Logistic regression analysis showed that preoperative high level of uric acid,preoperative high expression of LncRNA SBF2-AS1,the longer operation time and intraoperative hypotension were the risk factors for AKI after percutaneous nephrolithotomy in pa-tients with renal calculus(P＜0.05),while preoperative high level of VSIG4 was the protective factor(P＜0.05).The values of area under the curve of preoperative serum VSIG4 and LncRNA SBF2-AS1 in predicting AKI after percutaneous nephrolithotomy in patients with renal calculus were 0.854 and 0.705 respectively,and the area under the curve of combined prediction of the two inde-xes was 0.948,which was significantly higher than that of single index prediction(Z=1.995,2.958,P＜0.05).Conclusion Decreased preoperative serum VSIG4 level and increased expres-sion of LncRNA SBF2-AS1 in patients with renal calculus are associated with the occurrence of AKI after percutaneous nephrolithotomy,and the combined detection of preoperative VSIG4 and LncRNA SBF2-AS1 can predict the risk of AKI after operation.

Objective To investigate the relationships of preoperative serum V-set and immuno-globulin domain 4(VSIG4)and long chain non-coding ribonucleic acid(LncRNA)SBF2 antisense RNA1(SBF2-AS1)with acute kidneyinjury(AKI)after percutaneous nephrolithotomy in patients with renal calculus.Methods A total of 109 patients with renal calculus in the hospital from January 2020 to December 2022 were selected as research objects.Serum VSIG4 level and LncRNA SBF2-AS1 expression were detected in all the patients before operation,and incidence of AKI was recorded after operation.Multiple Logistic regression model was used to analyze the factors affecting AKI after percutaneous nephrolithotomy in patients with renal calculus;the receiver operating characteristic(ROC)curve was used to analyze the values of VSIG4 and LncRNA SBF2-AS1 in predicting AKI af-ter percutaneous nephrolithotomy in patients with renal calculus.Results In this study,16 cases had AKI after operation.The serum VSIG4 level in the AKI group was significantly lower than that in the non-AKI group,while the LncRNA SBF2-AS1 expression was significantly higher than that in the non-AKI group(P＜0.05).Multivariate Logistic regression analysis showed that preoperative high level of uric acid,preoperative high expression of LncRNA SBF2-AS1,the longer operation time and intraoperative hypotension were the risk factors for AKI after percutaneous nephrolithotomy in pa-tients with renal calculus(P＜0.05),while preoperative high level of VSIG4 was the protective factor(P＜0.05).The values of area under the curve of preoperative serum VSIG4 and LncRNA SBF2-AS1 in predicting AKI after percutaneous nephrolithotomy in patients with renal calculus were 0.854 and 0.705 respectively,and the area under the curve of combined prediction of the two inde-xes was 0.948,which was significantly higher than that of single index prediction(Z=1.995,2.958,P＜0.05).Conclusion Decreased preoperative serum VSIG4 level and increased expres-sion of LncRNA SBF2-AS1 in patients with renal calculus are associated with the occurrence of AKI after percutaneous nephrolithotomy,and the combined detection of preoperative VSIG4 and LncRNA SBF2-AS1 can predict the risk of AKI after operation.

ObjectiveTo investigate the factors related to the abandonment of upper limb prostheses. MethodsA total of 138 amputees fitting with upper limb prostheses at Guangdong Work Injury Rehabilitation Hospital from January 1st, 2016 to December 31st, 2022 were reviewed through case data, and those whose Functional Independence Measure scores improved after fitting were selected. A total of 126 amputees were investigated through telephone or WeChat with a questionnaire. The questionnaire was designed based on Trinity Amputation and Prosthesis Experience Scale-Revised, short form of Health Survey (SF-36) and World Health Organization Quality of Life Scale-Brief, and the items that may lead to the abandonment of upper limb prostheses were summarized in four factors: amputee, prosthetic fitting, prosthetic training, and environment and social policy. ResultsThe overall abandonment rate was 23.0% (29/126). There was significant difference between the the amputee abandonment or using prostheses in educational levels, amputation levels and residual limb condition (χ² > 6.808, P < 0.05); types of prostheses, whether functional prostheses, expectation for prosthesis, comfort of the prostheses, convenience of putting on and taking off, sensitivity of manipulation, weight of the prostheses, satisfaction with the appearance, skill levels of the prosthetist, the satisfaction after prosthesis installation and satisfaction with the prostheses in use (χ² > 13.083, P < 0.05); training levels before prosthesis assembly, the mastery of functional prostheses three months after prosthesis installation, training for activities of daily living, simulated training for vocation (χ² > 6.520, P < 0.05); willingness to participate in social activities with prostheses, attitude of other persons towards their prostheses, support of family members, work status, familiarity with the policies of welfare, and the current physical condition (χ² > 13.152, P < 0.01). ConclusionFactors of amputee, prosthetic fitting, prosthetic training, and environment and social policy may relate to the abandonment of upper limb prostheses, which are needed to improve in an accurate way.