1.The Prognostic Factors Of Diabetic Retinopathy Severity Among Type 2 Diabetes Mellitus In A Tertiary Teaching Hospital
Nur Jannah Ambak ; Nor Azwany Yaacob1, Siti Raihan Ishak
Malaysian Journal of Public Health Medicine 2016;16(2):48-54
Diabetic retinopathy (DR) may result in progressive visual impairment and blindness which affects the diabetic patients‟ morbidity and quality of life significantly. The objective of this study was to determine the proportions and prognostic factors of DR severity among Type 2 diabetes mellitus patients. A cross sectional study was conducted at the Ophthalmology Clinic in a tertiary hospital using the medical record database from 2005 to 2011. A total of 216DR patients were randomly selected. The study outcome was DR severity which was classified into four grading based on the International Clinical Diabetic Retinopathy Scale. Descriptive statistics and Ordinal Logistic Regression were applied using Stata SE/11. The mean (SD) age of DR in this study was 56.06 (9.98) years old with almost equal sex ratio. Proportion of mild non proliferative diabetic retinopathy (NPDR) was 48.6% (95% CI: 40.0, 60.0), moderate NPDR was 28.2% (95%CI: 22.0, 40.0), severe NPDR was 6.9% (95% CI: 4.0, 11.0) and proliferative diabetic retinopathy (PDR) was 16.2% (95%CI: 10.0, 22.0). Diabetic patients that suffered from diabetes mellitus for more than 10 years, having nephropathy, every increased of HbA1c by 1% and higher total cholesterol were at increased risk of worsening DR.
2.Molecular diversity pattern of intestinal lactic acid bacteria in Cemani chicken, Indonesian native chicken, as revealed by terminal restriction fragment length polymorphisms
Siti Nur Jannah ; Achmad Dinoto ; Komang Gede Wiryawan ; Iman Rusmana
Malaysian Journal of Microbiology 2016;12(1):102-111
Aims: An ecological study was conducted to investigate the diversity pattern of the lactic acid bacteria in the
gastrointestinal tract of Cemani chicken, a native Indonesian chicken, using a molecular approach based on 16S rRNA
genes.
Methodology and results: Digesta samples of seven chickens were collected for terminal restriction fragment length
polymorphism (T-RFLP) analysis. The molecular diversity of lactic acid bacteria in crop, ventriculus, ileum and cecum
were determined. The results showed that microbial composition of lactic acid bacteria in cecum was relatively different
with other upper gastrointestinal tract. Lactic acid bacteria phylotypes and diversity in ileum were higher than those in
the crop, ventriculus, and cecum.
Conclusion, significance and impact of study: We confirmed that cecum of native chicken has a different
environment as compared to other gastrointestinal regions showing the lowest value of the Sorensen’s index. This first
report of LAB diversity pattern in Cemani chicken contributes a more comprehensive understanding of the microbial
ecology in the chicken.
Gastrointestinal Tract