Aim: Nanotechnology is an increasingly growing field with its current application in Science and Technology for the purpose of manufacture of novel materials at the nanoscale level. Silver-Titania nanoparticles (AgTiO2-NPs) have been known to have inhibitory and bactericidal effects. Methodology and Results: In the present study, stable silver-titania nanoparticles coated metallic blocks were prepared for testing their efficacy against selected bacterial pathogens like Escherichia coli and Staphylococcus aureus. In the experimental part, the bacterial pathogens were inoculated on silver-titania nanoparticle coated blocks and the treatment was carried out in „0‟ time and „24‟ h interval and were enumerated. Conclusion, significance and impact of study:The results were compared with the control (uncoated metallic blocks) and analyzed by using Japanese Industrial Standard (JIS Z2801:2000) method. From this study, it was concluded that silver-titania nanoparticles has inhibitory effect on bacterial pathogen tested.