Systemic contact dermatitis (SCD) is a kind of allergic inflammatory skin disease caused by contact with the same or cross-reactive allergens via systemic absorption in individuals who have been exposed to allergens. SCD lesions are diverse and easily overlooked in clinical practice. This review summarizes progress in clinical manifestations, pathogenesis, diagnosis, treatment of and sensitizers in SCD, aiming to improve its clinical diagnosis and treatment.

Objective:To analyze clinical characteristics of drug-induced hypersensitivity syndrome (DIHS) , and to compare the European, Japanese and Chinese diagnostic criteria.Methods:A total of 45 patients confirmedly diagnosed with DIHS according to the DIHS criteria originally proposed by Bocquet, were collected from the First Affiliated Hospital (Southwest Hospital) of Army Medical University between January 2009 and January 2019. Clinical data on the 45 patients were retrospectively analyzed, clinical characteristics were summarized and re-evaluated according to the European, Japanese and Chinese diagnostic criteria separately, and differences were analyzed in terms of the latency period, time to rash regression, eosinophil count, liver function indices, etc. One-way analysis of variance was used to compare means among multiple groups, and t test to compare means between two groups. Results:Of the 45 patients, 38 presented with eruptive drug eruptions, and 44 were accompanied by liver damage, 40 by elevated counts of peripheral white blood cells, 38 by eosinophilia, 21 by lymphadenectasis, and 4 by mucosal damage. Common culprit drugs included allopurinol (10 cases) , anti-tuberculosis drugs (7 cases) , cephalosporins (7 cases) , and Chinese medicine (4 cases) . Forty patients were treated with glucocorticoids, and 17 with glucocorticoids and intravenous gamma globulin. After treatment, 44 patients received improvement and 1 died. According to the European diagnostic criteria, there were 29 patients with suspected DIHS and 16 with confirmed DIHS; according to the Japanese diagnostic criteria, 37 patients could be confirmedly diagnosed with DIHS, but 8 could not be confirmedly diagnosed; according to the Chinese diagnostic criteria, 17 patients could be confirmedly diagnosed, but 28 could not be confirmedly diagnosed. According to the Japanese diagnostic criteria, the latency period was significantly longer in the patients with a confirmed diagnosis (36.91 ± 21.73 d) than in those without (20.00 ± 20.82 d, P = 0.04) . Conclusions:Common culprit drugs for DIHS include allopurinol, anti-tuberculosis drugs and cephalosporins. Most patients with DIHS are accompanied by liver damage, and the European diagnostic criteria are preferentially recommended for DIHS.

Objective To explore the difference of gene expression profiling between normal basilar arteries and basilar arteries of cerebral vasospasm (CVS) after subarachnoid hemorrhage (SAH) in rabbits. Methods cDNA chip of normal basilar arteries and basilar arteries of CVS after SAH in rabbits were downloaded from GEO database. The chip was analyzed and screened by Bioconductor software, and function enrichment and pathway analysis of the differentially expressed genes were analyzed by Cytoscape software. Then 6 adult male Japanese rabbits were used, and randomly divided into normal control group (n=3) and SAH model group (n=3). Rabbit SAH models were established by cisterna secondary-blood-injection method. RNA data of normal basilar artery specimens on the 0 day and basilar artery specimens after SAH on the 5-day were used to validate the parts of differentially expressed genes by qRT-PCR. Results A total of 4356 differentially expressed genes were found in normal basilar arteries and basilar arteries of CVS after SAH in rabbits. Among them, 920 genes were considered to be significant with P-value<0.05, such as GRIK1, MYH13, ZNF45, SAA3, RLN1, MSR1 and others. Function enrichment analysis indicated that the differentially expressed genes were involved in regulation of Ca2+transmembrane transporter activity, negative regulation of ion transmembrane transport, regulation of potassium ion transport, positive regulation of JAK-STAT signaling cascades and other biological processes. Pathway analysis showed that calcium signaling pathway, cGMP-PKG signaling pathway, HIF-1 signaling pathway, PI3K-Akt signaling pathway and other signaling pathways maybe related with the differentially expressed genes. qRT-PCR verification showed that the expression of MSR1 in SAH model group was consistent with that of the chip result. Conclusion The gene expressions of basilar arteries of CVS after SAH in rabbits are significantly different, and MSR1 gene can be used as a potential target for studying the pathological mechanism of CVS.

Objective To investigate the effects of mirror visual feedback (MVF) and electromyographic biofeedback (EMGBF) on upper extremity function in hemiplegic patients after stroke based on task-oriented training. Methods 90 patients with hempiplegia after stroke were randomly divided into control group (n=30), EMGBF group (n=30) and MVF group (n=30). All patients accepted routine rehabilitation and task-oriented training once a day for 8 weeks. The EMGBF group also accepted EMGBF, and the MVF group accepted MVF in addition. They were assessed with Fugl-Meyer Assessment (FMA) and the Upper Extremity Function Test (UEFT), and their integrated electromyogram (iEMG) of affected upper extremities were recorded before and after treatment. Results All the groups improved in scores of FMA and UEFT, as well as the iEMG after treatment (P<0.05), and ranked as the MVF group, the EMGBF group and the control group from improving more to less (P<0.05). Conclusion Mirror visual feedback combined with electromyographic biofeedback may further promote the recovery of upper limb function in patients with hemiplegia after stroke based on task-oriented training.

With the rapid development of sequencing technologies towards higher throughput andlower cost, sequence data are generated at an unprecedentedly explosive rate. To provide an efficientand easy-to-use platform for managing huge sequence data, here we present Genome Sequence Archive (GSA; http://bigd.big.ac.cn/gsa or http://gsa.big.ac.cn), a data repository for archiving rawsequence data. In compliance with data standards and structures of the International Nucleotide Sequence Database Collaboration (INSDC), GSA adopts four data objects (BioProject, Bio Sample,Experiment, and Run) for data organization, accepts raw sequence reads produced by a variety of sequencing platforms, stores both sequence reads and metadata submitted from all over the world,and makes all these data publicly available to worldwide scientific communities. In the era of bigdata, GSA is not only an important complement to existing INSDC members by alleviating theincreasing burdens of handling sequence data deluge, but also takes the significant responsibilityfor global big data archive and provides free unrestricted access to all publicly available data insupport of research activities throughout the world.

Objective Toexploretheimagingcharacteristicsofileocecaltuberculosisbycontrast-enhancedCT.Methods TheCT findingsof16ileocecaltuberculosispatientswereretrospectivelyanalyzed.AllcasesunderwentplainandenhancedCTscan.Results 14casesshowedinhomogeneousthickeningoftheintestinalwallandnarrowingofthelumen,and2casesshowedextensiveanduniform thickeningoftheintestinalwallwithnoobviousnarrowingofthelumen.Theenhancedimagesshowedthat13caseswereobviously heterogeneousenhancement,2caseswerering-likeenhancement,and1casewashomogeneousenhancement.Blurredserosasurface wasseeninallpatients,withsurroundingexudationin14casesandperitoneuminvolvementin12cases.Inallcases,enlargedlymph nodeswerefoundneartheabdominalaortaandonmesentery,withhomogeneousenhancementin10cases,annularenhancementin5casesand calcificationin1case.Conclusion Ileocecaltuberculosisismainlycharacterizedbyinhomogeneousthickeningandheterogeneousenhancementofthe intestinalwall,aswellasnarrowingofthelumen.Thereisoftenobviousexudationaroundtheintestines.Ileocecaltuberculosisoften combineswithenlargedlymphnodesandintrapulmonarytuberculosis.TheenhancedCTscanisofgreatvaluefortheevaluationofileocecal tuberculosis.

Objective:To analyze and summarize clinical characteristics of atopic dermatitis (AD) , and to discuss differences and consistency among the Williams′, Japanese and Chinese diagnostic criteria for AD.Methods:A retrospective analysis was performed on 165 hospitalized patients with AD diagnosed according to the Williams′ criteria or the Chinese Zhang′s criteria in Southwest Hospital of Army Medical University from October 2000 to May 2020. Clinical data were re-evaluated according to the Williams′, Japanese and Chinese diagnostic criteria, and differences were analyzed among these criteria. Measurement data were compared by using t test, analysis of variance or rank sum test, and enumeration data were compared by using chi-square test or Fisher′s exact test. Results:Among the 165 patients, 66 (40.00%) developed AD before the age of 2 years; 95 (57.58%) had a personal and/or family history of atopic diseases, of whom 75 had a personal history of atopic diseases (mainly including allergic rhinitis [28.48%] and asthma[20.00%]) , 50 had a family history of atopic diseases, and 30 had both a personal and a family history of atopic diseases. A total of 98 patients (59.39%) were allergic to dust mites, including 48 with grade Ⅵ allergy. According to the total IgE level, 130 (78.79%) patients were diagnosed with exogenous AD, and 35 (21.21%) with endogenous AD. There were significant differences in the age at onset and blood eosinophil count between the patients with exogenous AD and those with endogenous AD (both P < 0.001) . After re-evaluation, 142 (86.06%) patients met the Williams′ criteria, 23 did not, and there was a significant difference in the age at onset between the two groups of patients ( P = 0.007) ; 150 (90.91%) patients met the Chinese Zhang′s criteria, 15 did not, and there was a significant difference in blood eosinophil counts between the two groups of patients ( P = 0.001) ; 160 (96.97%) patients met the Chinese Yao′s criteria; all patients met the Japanese criteria. There were no significant differences in the age at onset, total IgE level or blood eosinophil counts among patients meeting the Williams′ criteria, meeting the Japanese criteria, meeting the Zhang′s criteria, and those meeting the Yao′s criteria (all P > 0.05) . Conclusion:Patients with early-onset AD were prone to develop the highest-grade dust mite allergy; patients with exogenous AD were characterized by lower age at onset and higher eosinophil counts compared with those with endogenous AD; patients who met the Williams′ criteria showed lower age at onset than those who did not, while patients who met the Chinese Zhang′s criteria showed higher eosinophil counts than those who did not.

Background: Studies have shown that abnormal expression of circular RNA (circRNA) is closely related to the development, progress and prognosis of a variety of tumors, and is an ideal diagnostic indicator and therapeutic target. However, the role of circRNA in the development and progress of pancreatic cancer needs to be further explored. Aims: To investigate the expression of circ-RANBP1 in pancreatic cancer tissue and its effect on pancreatic cancer cell proliferation, migration and invasion. Methods: The expression of circ-RANBP1 in pancreatic cancer tissue and normal para-cancerous tissue was detected by in situ hybridization. MIA PaCa-2 cells and SW 1990 cells were cultured, and transfected with knockdown oligomer and overexpressed plasmid of circ-RANBP1, respectively, and corresponding control groups were served. Expression of circ-RANBP1 in pancreatic cancer cells was detected by qRT-PCR. EdU method was used to detect the effect of circ-RANBP1 on cell proliferation. Transwell assay was used to detect the effect of circ-RANBP1 on cell migration and invasion. Western blotting and immunofluorescence were used to detect the effect of circ-RANBP1 on epithelial-mesenchymal transition (EMT). Angiogenesis assay was used to explore the effect of circ-RANBP1 on angiogenesis ability. Results: The expression of circ-RANBP1 was significantly increased in pancreatic cancer tissue when compared with paired normal tissues, and was closely associated with poor prognosis of patients. Circ-RANBP1 knockdown inhibited the proliferation of MIA PaCa-2 cells, while overexpression of circ-RANBP1 promoted the proliferation of SW 1990 cells. Compared with control group, circ-RANBP1 knockdown suppressed the migration and invasion of MIA PaCa-2 cells, and overexpression of circ-RANBP1 promoted the migration and invasion of SW 1990 cells. Knockdown of circ-RANBP1 could inhibit EMT, while circ-RANBP1 overexpression showed opposite effect. Inhibition of circ-RANBP1 significantly reduced angiogenesis, while overexpression of circ-RANBP1 significantly enhanced angiogenesis. Conclusions: Circ-RANBP1 is highly expressed in pancreatic cancer tissue, and can promote the proliferation, migration, invasion, EMT and angiogenesis of pancreatic cancer cells.

With the deepening of the reform of the medical and health system and the continuous optimization of the medical order,it is especially important to organize the development of admission and discharge standards and improve the service of preparing patients for discharge.In recent years,the research and application of machine learning technology in the medical field has been intensifying,and it has unique advantages in processing data and risk prediction research.Therefore,this paper reviews the development process,types of machine leaming,the content and effects of its application in patient discharge preparation services,and the current problems,in order to provide references for healthcare professionals to implement the best clinical decisions and further improve the patient discharge preparation service model.

Objective:To compare clinical characteristics of patients with atopic prurigo nodularis (APN) versus non-APN (NAPN), and to analyze differences in the therapeutic response to dupilumab.Methods:A retrospective study was conducted. Clinical data were collected from patients with atopic dermatitis and those with prurigo nodularis, who visited the Department of Dermatology, Southwest Hospital, Army Medical University from January 2017 to December 2022. These patients were divided into the APN group and NAPN group according to the inclusion and exclusion criteria (types of skin lesions and the presence or absence of history of atopic diseases). Clinical characteristics, laboratory examination results, and comorbidities were compared between the 2 groups, so were the eczema area and severity index (EASI) scores, investigator′s global assessment (IGA) scores and itch numeric rating scale (NRS) scores at 0, 4, 12 weeks after the treatment with dupilumab.Results:A total of 233 patients with NAPN and 177 with APN were enrolled, and their ages were 57 (48, 68) and 43 (20, 57) years, respectively. Before the treatment, the serum IgE levels and eosinophil counts were significantly higher in the APN patients than in the NAPN patients ( Z = -4.40, -3.92, respectively, both P < 0.001) ; compared with the NAPN patients, the APN patients more frequently presented with skin lesions on the trunk (71.64% [117/177] vs. 54.08% [126/233], P < 0.05), limbs (71.75% [127/177] vs. 61.37% [143/233], P < 0.05) and the whole body (28.36% [53/177] vs. 20.17% [47/233], P < 0.05) ; the incidence rates of xeroderma, erythema, papules and crusting were significantly higher in the APN patients than in the NAPN patients (all P < 0.05), and the APN patients more frequently presented with symmetrically distributed lesions; however, there were no significant differences in the incidence rates of secondary lesions such as scratches (38.98% [67/177] vs. 33.91% [79/233]) and ulcers (18.64% [33/177] vs. 18.03% [42/233]) between the two groups (both P > 0.05). The proportions of patients with chronic kidney diseases, cardiovascular diseases, liver diseases and diabetes were significantly higher in the NAPN group than in the APN group (all P < 0.05). There were 13 patients with APN and 5 with NAPN who received a 12-week dupilumab treatment and had complete follow-up results. No significant differences were observed in the EASI, IGA or NRS scores before the treatment between the 2 groups (all P < 0.05) ; after the 12-week treatment, the improvement of EASI score was significantly higher in the APN group than in the NAPN group ( P < 0.05), while there were no significant differences in the improvement of NRS and IGA scores between the 2 groups (both P > 0.05) . Conclusion:The clinical manifestations, laboratory examination results, and comorbidities differed between the NAPN and APN patients, however, they both showed good therapeutic response to dupilumab.