1.Identification of pathogenic bacteria on the salted fish Lutjanus vivanus in Sorong City of West Papua
Sukmawati ; Fatimah Hardianti ; Sipriyadi ; Isna Rasdianah Aziz
Malaysian Journal of Microbiology 2019;15(3):237-244
Aims:
Fisheries-based food is needed by humans as a protein source, one of which is salted fish Lutjanus vivanus. Market demand for this product in Indonesia is quite high. The aim of the study was to identify pathogenic bacteria in salted fish L. vivanus.
Methodology and results:
The method used in this study was descriptive method including pathogenic test, bacterial genomic DNA isolation based on 16 sRNA gene using prokaryotic specific primers, namely 63f forward primer (5′-CAG GCC TAA CAC ATG CAA GTC-3′) and reverse 1387r primer (5′-GGG CGG WGT GTA CAA GGC-3′). The results of this study indicated pathogenic bacteria in salted fish L. vivanus with pathogenic activity of α hemolytic and β hemolytic. The bacteria were identified as Serratia marcescens strain ZK2 16S for isolate KS and Bacillus altitudinis strains A-19 16S for isolate LG.
Conclusion, significance and impact of study
In salted fish L. vivanus in the Sorong city, West Papua, it was obtained Serratia marcescens strain ZK2 16S with total hemolytic activity and B. altitudinis A-19 16S strain with partial hemolytic activity.
2.Identification bacteria associated with Haliclona sp. sponges from Enggano Island, Indonesia with antimicrobial activity against human pathogens
Sipriyadi ; Uci Cahlia ; Welly Darwis ; Risky Hadi Wibowo ; Enny Nugraheni ; Mardhatillah Sariyanti
Malaysian Journal of Microbiology 2021;17(2):178-189
Aims:
Antimicrobial compounds are bioactive compounds that have ability to inhibit microbial growth activities. This
study aimed to screen and identify bacteria associated with Haliclona sp. sponges from Enggano Island, Indonesia that
had potential to produce antimicrobial compounds against Escherichia coli, Candida albicans and Staphylococcus
epidermidis.
Methodology and results:
The method used to screen and identify bacteria in this study including screening assay,
morphological identification, Gram staining and spore staining method, biochemical tests and molecular identification
based on 16S rRNA gene. This study resulted 16 isolates which were successfully isolated from Haliclona sp. According
to screening assay, 5 isolates could potentially produce antimicrobial compounds coded as HEBS1, HEBS3, HEBS6,
HEBB2 and HEBB3. Based on Gram staining, spore staining, biochemical test and molecular identification results,
HEBS1 had proximity to Brachybacterium paraconglomeratum, HEBS3 had proximity to Kocuria palustris, HEBS6 had
proximity to Psychrobacter pasificensis, HEBB2 had proximity to Bacillus aryabhattai, and HEBB3 had proximity to
Bacillus toyonensis.
Conclusion, significance and impact of study
From 16 isolates that successfully isolated, there were 5 isolates that
could potentially produce antimicrobial compounds against Escherichia coli, Staphylococcus epidermidis and Candida
albicans. These isolates can be served as antimicrobial compounds producer. However, identification and purification of
these antimicrobial compounds are needed to be done before applied it for medicine in the future.
Haliclona--microbiology
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Host-Pathogen Interactions