AIM: The fingerprint chromatograms were established for quality evaluation of Desmodium styracifolium collected from 11 areas by HPLC. METHODS: The analysis was performed on a Phenomsil C_(18) column((4.6 mm)?250 mm,5 ?m) with acetonitrile-carbinol-water as mobile phase in a gradient mode at a flow rate of(1.0) mL/min,and at a column temperature of 25℃.The detection of wavelength was at 272 nm. RESULTS: 11 peaks were selected as the common fingerprint peaks.The relative standard deviations for relative retention values and relative peak areas were less than 3% in the precision and repeated test,the similarity of 11 batches of samples were no less than 0.9. CONCLUSION: The method is reliable and can be helpful on the quality control of Desmodium styracifolium.

Objective To analyze the situation and study trend of large-scale nurses'health cohort studies based on Web of Science,to help design clinical guidelines and scientific research for nursing staff in China.Methods Articles of large nurses'health cohort studies published from 1993 to 2023 were retrieved from Web of Science(WOS)Core Collection.Citespace and Bibilometrix were used to perform the bibliometric analysis.Results 2882 studies were included in this study.Total volume of papers was enlarging by year.Clustering results showed that the main keywords could be divided into 3 categories:risks of women's health,risks of cardiovascular disease and reproducibility.Results of keywords burst showed that lifestyle,cardiovascular disease,women reproductive health and mental health were the highlights of studies in recent 5 years.Conclusion Research on large-scale nurse health cohorts holds significant scientific significance.It is recommended to establish a nationwide large-scale nurse health cohort as soon as possible.In the study,appropriate exposure factors should be selected,with reproductive health,mental health,and psychiatric disorders being the primary outcome measures,and chronic physical illnesses being the secondary outcome measures.By systematically describing the factors influencing the physical and mental health of nurses in China,we can better safeguard their well-being and promote the development of the nursing discipline.