No abstract available.
Simplexvirus* ; Tinea*

No abstract available.
Acyclovir* ; Herpes Simplex* ; Simplexvirus*

No abstract available.
Herpes Simplex* ; Simplexvirus*

No abstract available.
Herpes Genitalis* ; Herpes Simplex* ; Simplexvirus*

OBJECTIVETo quantitatively evaluate the contamination area and risk of a live pathogen during tissue homogenization by either ultrasonic processor or tissue disperser.

METHODSA recombinant Herpes Simplex Virus (rHSV) containing GFP gene was used as the index virus, and fresh liver tissue from healthy mice was used as simulated specimen. After 10% liver homogenate was mixed with rHSV (100 TCID50/0.1 mL) in a 5 mL tube, the stability of rHSV in liver homogenate and influences of an ultrasonic processor and a tissue disperser on viral infectivity were determined by GFP expressions in cell cultures. The contaminating areas of live viruses during homogenization were evaluated by a cell culture-based sedimentary. The contamination radii were counted by measurement of the distance between the operator and the farthest GFP positive well.

RESULTSThe infectivity of rHSV in 10% liver homogenate maintained almost unchanged after it was incubated at room temperature for 30 min. Treatment with an ultrasonic processor clearly dropped down the virus infectivity, while a disperser not. Obvious spills and slashes of live viruses were observed in processes of homogenization with those two apparatuses. The contamination radii are positively related with sample volume, output energy of operator and handling time.

CONCLUSIONHomogenizing infectious samples with an ultrasonic processor and a tissue disperser at commonly used conditions caused obvious spills and splashes of live viruses, which possesses high risk to induce Laboratory acquired infections (LAIs).

Adenoviridae ; Humans ; Simplexvirus ; Thymidine Kinase

No abstract available.
Herpes Simplex ; Simplexvirus ; Skin Diseases

Cutaneous infection by cytomegalovirus (CMV) is rare, and its clinical manifestations vary from purpuric papules to nodules, plaques, vesicles, bullaes, and ulcers. Cutaneous CMV is difficult to diagnose, clinically as well as histopathologically, because the microscopic changes induced by CMV are easily overlooked due to the sparcity of the typical change. We report a case of coinfection of the herpes simplex virus and CMV in a renal transplant patient. Knowledge of the histopathologic features of CMV infection helped us to diagnose it promptly and to start early treatment.
Coinfection ; Cytomegalovirus Infections* ; Cytomegalovirus* ; Humans ; Simplexvirus ; Ulcer

No abstract available.
Bronchoalveolar Lavage ; Herpes Simplex* ; Pneumonia ; Simplexvirus*

No abstract available.
Herpes Simplex* ; Humans ; Lasers, Excimer* ; Simplexvirus* ; Vitiligo*