Objective:To explore therapeutic effect and safety of cilostazol combined clopidogrel +aspirin on aged pa‐tients after percutaneous coronary intervention (PCI) .Methods :A total of 100 aged patients with coronary heart disease undergoing PCI were randomly divided into routine treatment group (n=52 ,received aspirin and clopidogrel antiplatelet therapy) and cilostazol group (n= 48 ,received cilostazol therapy based on routine treatment group ) . Turbidimetry was used to measure platelet aggregation rate (PAR );PAR and mean platelet volume (MPV ) were compared between two groups before ,one week and one month after PCI .All subjects were followed up for six months ,incidence rates of major adverse cardiovascular events (MACE) and hemorrhage events were compared be‐tween two groups .Results:There were no significant difference in MPV and PAR between two groups before PCI . Compared with routine treatment group ,PAR significantly reduced [one week after PCI :(48.7 ± 6.3)% vs .(43.5 ± 5.7)% ,one month after PCI :(46.8 ± 5.8)% vs .(42.4 ± 5.4)% ] in cilostazol group , P<0.05 both . After six-month follow-up ,there was no significant difference in total incidence rate of MACE (16.7% vs .17.3% ) be‐tween cilostazol group and routine treatment group , P>0.05;incidence rate of hemorrhage in cilostazol group was significantly lower than that of routine treatment group (6.25% vs .19.23% , P< 0.01) .Conclusion:Compared with clopidogrel+aspirin therapy ,cilostazol combined clopidogrel+aspirin can more significantly inhibit platelet ag‐gregation ,and significantly reduce hemorrhage events ,so they are safe and effective in aged patients with coronary heart disease undergoing PCI .

Objective To investigate the effect of tMfn2 gene on inhibiting the proliferation of vascular smooth muscle cells (VSMCs) and related mechanism. Method VSMCs were transfected with adenovirus vector encoding tMfn2 or Mfn2 (Adv-tMfn2, Adv-Mfn2). The abundance of tMfn2 protein and Mfn2 protein were deter-mined by Western blot analysis using Mfn2 N-term antibody. The effect of tMfn2 on the proliferation of VSMCs was explored by cell counting and MTT assay. The cell cycle was analyzed using flow cytometry. Western blot were used to detect the expression of p-ERK1/2 and p-Raf-1. Results The results of cell counting and MTT both indi-cated that tMfn2 gene displayed more remarkable effect on inhibiting the proliferation of VSMCs than Mfn2 (P <0.01). Flow cytometry showed that most of the cells infected with Adv-tMfn2 or Adv-Mfn2 were blocked in the stage of G0/G1 and few entered into the S phase. Western blot indicated that overexpression of tMfn2 gene resulted in downregulation of phosphorylated ERK1/2 and Raf-1 protein (P < 0.01). These results demonstrated tMfn2 had stronger effect than Mfn2 (P < 0.01). Conclusions tMfn2 gene is superior to Mfn2 gene in attenuating the proliferation of VSMCs via the Ras-Raf-ERK1/2 signaling pathway.

Objective: To establish a method for the simultaneous determination of geniposide, liquiritin, aloe-emodin, rhein, emodin, chrysophanol and physcion in Yinzhi Quhuang capsules by an HPLC wavelength switching method. Methods:A Waters Sun-fire C18(250 mm ×4.6 mm,5μm) column was used with the mobile phase of methanol-0.05% phosphoric acid solution (gradient elu-tion) at a flow rate of 1. 0 ml·min-1 , the column temperature was 25℃, the detection wavelength was 238 nm in 0-15 min for genipo-side and liquiritin, and 254 nm in 15-50 min for aloe-emodin,rhein, emodin, chrysophanol and physcion. The injection volume was 10μl. Results: The linear range was 0.13-3.18 μg·ml-1 (r =0.9998) for geniposide, 0.19-4.84 μg·ml-1(r =0.9997) for liquiritin, 0. 28-7. 02μg·ml-1(r=0. 9999) for aloe-emodin, 0. 13-3. 16μg·ml-1(r=0. 9999) for rhein, 0. 61-15. 27μg·ml-1 (r=0.9999) for emodin, 0.32-8.03 μg·ml-1(r=0.9999) for chrysophanol and 0.39-9.81 μg·ml-1(r=0.9999) for phy-scion. The average recovery was 98. 84%(RSD=0. 74%), 99. 34%(RSD=0. 86%), 99. 54%(RSD=0. 30%), 99. 56% (RSD=0. 80%), 99. 85% (RSD=0. 41%), 99. 57% (RSD=0. 70%) and 99. 64% (RSD=0. 30%) (n=9). Conclusion: The deter-mination method has the advantages of simple operation, high specificity, good reproducibility and accurate results, which can be used for the quality control of Yinzhi Quhuang capsules.