Objective To explore the effect of early sedative in prevention of febrile convulsion epilepsy in children. Methods A total of 146 children with febrile convulsion epilepsy who were treated in our hospital were selected as the researchobjects. According to the random number table method, they were randomly divided into control group and research group, with 73 cases in each group. The control group was treated with routine treatment such as symptomatic cooling, while the study group was treated with sedatives at the early stage. After 6 months, the clinical symptoms and preventive effects of the two groups were compared. Results After treatment, the incidences of epilepsy, brain injury and mental retardation in the study group were significantly lower than that in the control group (P ＜ 0. 05), and the cure rate in the study group was significantly higher, and recurrence rate was significantly lower than that of the control group (P ＜ 0. 05). Conclusion Sedatives are effective in the treatment of early febrile convulsions and epilepsy in children. It can alleviate the symptoms, effectively prevent the seizures caused by febrile convulsions and other complications, and reduce the recurrence rate.

Objective To explore the effect of early sedative in prevention of febrile convulsion epilepsy in children. Methods A total of 146 children with febrile convulsion epilepsy who were treated in our hospital were selected as the researchobjects. According to the random number table method, they were randomly divided into control group and research group, with 73 cases in each group. The control group was treated with routine treatment such as symptomatic cooling, while the study group was treated with sedatives at the early stage. After 6 months, the clinical symptoms and preventive effects of the two groups were compared. Results After treatment, the incidences of epilepsy, brain injury and mental retardation in the study group were significantly lower than that in the control group (P ＜ 0. 05), and the cure rate in the study group was significantly higher, and recurrence rate was significantly lower than that of the control group (P ＜ 0. 05). Conclusion Sedatives are effective in the treatment of early febrile convulsions and epilepsy in children. It can alleviate the symptoms, effectively prevent the seizures caused by febrile convulsions and other complications, and reduce the recurrence rate.

OBJECTIVE To study the cardiotoxicity of ophiopogonin D′(OPD′) for rat H9c2 cardio? myocytes. METHODS H9c2 cells were exposed to OPD′ 0.1, 1, 5, 10, 20, 25 and 50 μmol·L-1 for 24 h. Cell viability was examined by MTS assay, and the morphological changes in H9c2 cells were quanti? fied. The cell nucleus injury was examined by high content immune fluorescence screening and the morphological changes were observed under a fluorescence microscope. After treatment with OPD′ 0.1, 1, 5 and 10 μmol·L- 1 for 24 h, the effect on reactive oxygen species (ROS), mitochondrial mem? brane potential(MMP) and apoptosis was detected by flow cytometry. RESULTS The viability was sig? nificantly reduced following exposure to OPD′ 0.1, 1, 5, 10, 20, 25 and 50 μmol·L- 1 (P<0.05,P<0.01). The IC50 value was 9.9 μmol ·L- 1 and cell shrinkage and apoptosis occurred. The levels of ROS and apoptosis rate of H9c2 cells were significantly increased after exposure to OPD′ 0.1, 1, 5 and 10 μmol·L-1 for 24 h (P<0.05,P<0.01) and MMP markedly declined (P<0.05,P<0.01). CONCLUSION OPD′ has significent cytotoxicity on H9c2 cells. It may be related to inducing apopotsis pathways.

Objective:To investigate the correlation of serum tartrate-resistant acid phosphatase 5b (TRAP-5b), β-collagen degradation products (β-CTX), vitamin D levels and rheumatoid arthritis (RA) complicated with osteoporosis (OP) fractures.Methods:A total of 200 RA patients diagnosed and treated in our hospital from Oct. 2022 to Oct. 2023 were selected. Clinical data of patients were collected, and serum TRAP-5b, β-CTX, vitamin D levels, bone mineral density and bone metabolism markers were detected. They were divided into RA group and RA-OP group according to whether OP fracture occurred. The above indexes were compared and their correlation with osteoporotic fractures in rheumatoid arthritis was analyzed.Results:The levels of serum TRAP-5b and β-CTX in RA patients were higher than those in healthy control group, and the levels of vitamin D were lower ( t=12.27, 20.01, 7.35, P<0.05). There was no statistically significant difference in gender, body mass index, diabetes, hypertension, smoking and drinking incidence between the two groups ( t=1.18, 0.96, 0.55, 1.84, 0.13, 0.30, P>0.05), but there was statistically significant difference in gender composition, serum TRAP-5b, β-CTX, vitamin D and bone metabolism markers between the two groups ( t=2.33, 3.72, 4.94, 5.10, 7.72, 4.77, 2.42, 9.30, 4.01, 6.38, 4.79, P<0.05). Logistic regression analysis showed that the levels of serum TRAP-5b, β-CTX, vitamin D and bone metabolism markers were independent risk factors for OP in RA patients ( P<0.05). Serum TRAP-5b and β-CTX were positively correlated with serum PINP, NTx, PTH, BALP and BGP, respectively ( r=0.811, 0.778, 0.747, 0.675, 0.614; 0.685, 0.513, 0.405, 0.612, 0.518), and negatively correlated with lumbar BMD and proximal femoral BMD ( r = -0.488, -0.512; -0.526, -0.502) ; Serum vitamin D was negatively correlated with serum PINP, NTx, PTH, BALP, BGP ( r=-0.728, -0.640, -0.604, -0.622, -0.545), and positively correlated with lumbar BMD and proximal femur BMD ( r=0.519, 0.492) ( P<0.05) . Conclusions:Serum levels of TRAP-5b, β-CTX and vitamin D are closely related to the occurrence of OP in RA patients. Abnormal increase of serum levels of TRAP-5b and β-CTX and abnormal decrease of vitamin D suggest an increasing risk of OP in RA patients.

Objective:To explore the effect of neutrophil elastase(NE)on neutrophil inflammatory recruitment.Methods:Mice bone marrow-derived neutrophils were pretreated with an exogenous elastase inhibitor-sivelestat sodium.The effects of elastase inhibition on the in vivo inflammatory recruitment,chemotaxis,adhesion,cell polarization and spreading of NE were examined by peritonitis adoptive transfer assay,dunn chamber,flow chamber,immunofluorescence staining and spreading assay,respectively.The effects of elastase inhibition on NE phagocytosis and reactive oxygen species(ROS)release capacity were detected by flow cytom-etry and the luminol chemiluminescence system.Results:Sivelestat sodium pretreatment significantly attenuated neutrophil in vivo in-flammatory recruitment(P<0.001);impaired neutrophil perception of chemotaxis in vitro(P<0.05),slowed chemotactic velocity(P<0.05),and decreased the chemotactic distance(P<0.05);reduced the adhesion of neutrophils to inflamed endothelial cells(P<0.000 1)and inhibited the phagocytosis of bacteria by neutrophils(P<0.01);however,there was no significant effect on neutrophil spreading,polarization and ROS.Conclusion:NE inhibition significantly impaired the inflammatory recruitment cascade response and phagocyto-sis of neutrophils in vitro and in vivo but had no significant effect on the spreading,polarization and ROS release of neutrophils.

Objective:To summarize the clinical manifestations, imaging characteristics, and diagnoses basis of adrenomyeloneuropathy (AMN).Methods:The clinical data of 3 patients with AMN, admitted to our hospital from November 2016 to April 2019, were retrospectively collected. The clinical manifestations, imaging features, and diagnostic process of these patients were analyzed.Results:Three young male patients had onset with gradual aggravation of unilateral or bilateral lower limb insufficiency. MR imaging showed symmetrical abnormal signals in brainstem in 2 patients, and atrophy of thoracic spinal cord in 1 patient. By target region capture sequencing, mutations in the ABCD1 gene were found in all 3 patients; 2 underwent pedigree validation; the remaining one patient and his mother had failed Sanger sequencing validation due to pseudogene interference, and elevated plasma level of very long chain fatty acid (VLCFA) was noted in this patient. Conclusions:AMN usually initiates in the adulthood with spastic paraplegia as onset. Symmetrical lesions in brainstem or atrophy of spinal cord can be manifested on MR imaging; some patients may be accompanied by adrenocortical insufficiency. The definite diagnosis mainly depends on genetic screening and determination of VLCFA level in the blood.

The immune related adverse events(irAE)caused by tumor immune checkpoint inhibitors(ICI)have attracted increasing attention of clinical experts.Immune-mediated liver injury caused by ICIs(ILICI)is not uncommon in clinical practice,but specific diagnostic method of ILICI is lacking.Biopsy of liver tissue can help improve the diagnosis and management of ILICI.In the treatment of ILICI,the immediate use of corticosteroid therapy is not necessarily.A balance between efficacy,toxicity,and specific treatment need to be achieved,and further refined through multidisciplinary team(MDT)cooperation.Appropriate dosaging and identification of novel predictive targets should be considered in order to reduce the incidence and severity of ILICI in the future.Meanwhile,further basic research is required to elucidate the potential pathophysiological mechanisms and risk factors of ILICI.With the refinement of evidence in clinical evidence-based medicine and deepening of basic research,the diagnosis and treatment level of ILICI will also be further improved.