OBJECTIVETo develop a HPLC method for the determination of synephrine and arecoline contents in Xiao'er Xiaoji Zhike oral liquid.
METHODThe analysis was performed on a Symmetry C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile-methanol-0.1% acetic acid solution (containing potassium dihydrogen phosphate 0.6 g and SDS 1.0 g per 1 000 mL) (15: 30: 55) as mobile phase. The detection wavelength was set at 215 nm.
RESULTThe synephrine and arecoline concentrations had good linear relationship between 0.281 5-2.815 and 0.040 16-0.401 6 microg (r > 0.999 7), and the average recoveries of the two compounds were 97.1% (RSD 1.4%) and 100% (RSD 1.3%), respectively.
CONCLUSIONThe method is simple, accurate and sensitive.
Arecoline
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analysis
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Chromatography, High Pressure Liquid
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methods
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Drugs, Chinese Herbal
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analysis
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Synephrine
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analysis