Objective To detect the expression change of ETFβin diabetic nephropathy rats and study the role of ETFβin fatty acid-induced apoptosis in renal tubules.Methods Diabetic nephropathy model was established by intraperitoneal injection of streptozotocin and unilateral nephrectomy.In vivo ETFβexpression was detected in renal cortex, as well as tubular injury evaluated.In vitro fatty acid-induced apoptosis in renal tubular cells NRK 52E model was established and ETFβrecombinant plasmid was constructed to be transfected into NRK 52E cells and furtherly to observe the effect of ETFβover-expression on the fatty acid-induced apoptosis.Results In the rats model of diabetic nephropathy induced by streptozotocin injection and unilateral nephrectomy, ETFβmRNA and protein expression were decreased as obvious tubular damage occurred.Fatty acids could induce apoptosis in NRK 52E, and ETFβover-expression reduced the apoptosis.Conclusion The expression of ETFβis decreased in diabetic nephropathy model , and ETFβover-expression can reduce apoptosis induced by fatty acid in renal tubular cells.

Objective: To explore associated factors of mobile phone dependence and its relation with psychological resilience among college students. Methods: College students from 5 universities in Hefei were randomly selected through multi-stage sampling (stratified clustering) and investigated with questionnaires. A total of 2 502 college students were included in the analysis. Results: Mobile phone dependence among college students differed by gender (χ2=18.25, P<0.01), residence (χ2=17.71, P<0.01), whether in a relationship(χ2=8.09, P<0.01), grade(χ2=19.58, P<0.01), only child(χ2=7.48, P<0.01), family economic status (χ2=17.43, P<0.01) and time spent in mobile phone (χ2=73.46，P<0.01) while no similar differences were found by family structure and length of mobile phone ownership. Spearman correlation showed negative correlation (P<0.01) between mobile phone dependence and psychological resilience. Logistic regression model results showed that female, not in a relationship, lower grade, less time spent in mobile phone and high psychological resilience were negatively correlated with mobile phone dependence. Compared with students from rural areas, urban area was positively associated with mobile phone dependence. Emotional control, family support, and interpersonal assistance associated with lower risk for mobile phone dependence. Conclusion Mobile phone dependence is affected by gender, relationship status, grade, usage duration, and residence. High psychological resilience associated negatively with risk for mobile phone dependence.

Objective:To establish a prediction model of risk factors for early Q-T interval prolongation after acute myocardial infarction (AMI), which helps prevent and reduce the occurrence of acute malignant events.Methods:This is a case-control study. A total of 100 patients with Q-T interval prolongation after AMI who received treatment at Heilongjiang Provincial Hospital from January 2018 to December 2022 were included in this study. An additional 100 patients without Q-T interval prolongation after AMI who concurrently received treatment in the same hospital were also included in this study. Two model groups, including model group 1 (with Q-T interval prolongation, n = 50) and model group 2 (without Q-T interval prolongation, n = 50), and two test groups, including test group 1 (with Q-T interval prolongation, n = 50) and test group 2 (without Q-T interval prolongation, n = 50), were designated. Logistic regression analysis was performed to construct a prediction model of risk factors for Q-T interval prolongation. The area under the receiver operating characteristic curve was determined to evaluate the prediction model. The value of the prediction model was validated in the test groups. Results:Multivariate logistic regression showed that female gender ( OR = 2.307, 95% CI: 0.09-0.91, P = 0.041) and heart failure ( OR = 3.087, 95% CI: 1.15-8.27, P = 0.025) were independent risk factors for early Q-T interval prolongation after AMI. The area under the receiver operating characteristic curve of the prediction model was 0.770, with a sensitivity of 84.0%, a specificity of 66.0%, the Jordan index of 0.44, and the corresponding optimal critical value of 0.43. This indicates good fit of the model. Conclusion:Female gender and heart failure are independent risk factors for early Q-T interval prolongation after AMI. The model constructed based on the above-mentioned risk factors fits well and has a high predictive value, which helps reduce the occurrence of early Q-T interval prolongation after AMI.

Objective To analyze the research literature on Abelmoschus manihot utilizing the CiteSpace visualization software and summarize the current research hotspots and trends in this field.Methods Relevant literature relating Abelmoschus manihot was retrieved from the China National Knowledge Infrastructure(CNKI)database.The CiteSpace 5.7.R5 software was employed to conduct a visual analysis of researchers,research institutions,and key words in the selected literature that met the inclusion criteria.Results A total of 319 relevant articles were retrieved,of which 284 articles were ultimately included for analysis.The earliest research on Abelmoschus manihot could be traced back to 1979.Core authors included LIU Zhihui,TAN Xianhe,ZHU Huayun,etc.Institutions with a higher volume of publications included Nanjing University of Chinese Medicine,the Affiliated Hospital of Nanjing University of Chinese Medicine,Anhui Medical University,Jiangsu Suzhong Pharmaceutical Group,and Jiangsu Provincial Hospital of Traditional Chinese Medicine.Research hotspots encom-passed active ingredients,empirical prescriptions,oxidative stress,gut microbiota,Crohn's disease,calcium overload,component identification,and preparation processes.Conclusion The visual anal-ysis of Abelmoschus manihot research presented in the form of knowledge maps reveals that current research spans multiple disciplines.However,collaboration among research institutions needs to be strengthened.

Objective To analyze the research literature on Abelmoschus manihot utilizing the CiteSpace visualization software and summarize the current research hotspots and trends in this field.Methods Relevant literature relating Abelmoschus manihot was retrieved from the China National Knowledge Infrastructure(CNKI)database.The CiteSpace 5.7.R5 software was employed to conduct a visual analysis of researchers,research institutions,and key words in the selected literature that met the inclusion criteria.Results A total of 319 relevant articles were retrieved,of which 284 articles were ultimately included for analysis.The earliest research on Abelmoschus manihot could be traced back to 1979.Core authors included LIU Zhihui,TAN Xianhe,ZHU Huayun,etc.Institutions with a higher volume of publications included Nanjing University of Chinese Medicine,the Affiliated Hospital of Nanjing University of Chinese Medicine,Anhui Medical University,Jiangsu Suzhong Pharmaceutical Group,and Jiangsu Provincial Hospital of Traditional Chinese Medicine.Research hotspots encom-passed active ingredients,empirical prescriptions,oxidative stress,gut microbiota,Crohn's disease,calcium overload,component identification,and preparation processes.Conclusion The visual anal-ysis of Abelmoschus manihot research presented in the form of knowledge maps reveals that current research spans multiple disciplines.However,collaboration among research institutions needs to be strengthened.

OBJECTIVE: To assess the value of copy number variation sequencing (CNV-seq) for the diagnosis of children with intellectual disability (ID), developmental delay (DD), and autistic spectrum disorder (ASD). METHODS: Forty patients with ID/DD/ASD referred to Nanshan Maternity and Child Health Care Hospital from September 2018 to January 2022 were enrolled. G-banded karyotyping analysis was carried out for the patients. Genomic DNA was extracted from peripheral blood samples and subjected to CNV-Seq analysis to detect chromosome copy number variations (CNVs) in such patients. ClinVar, DECIPHER, OMIM and other database were searched for data annotation. RESULTS: Among the 40 patients (including 30 males and 10 females), 16, 15 and 6 were diagnosed with ID, DD and ASD, respectively. One patient had combined symptoms of ID and DD, whilst the remaining two had combined ID and ASD. Four patients were found with abnormal karyotypes, including 47,XY,+mar, 46,XY,inv(8)(p11.2q21.2), 46,XX,del(5)(p14) and 46,XX[76]/46,X,dup(X)(p21.1q12). Chromosome polymorphism was also found in two other patients. CNV-seq analysis has detected 32 CNVs in 20 patients (50.0%, 20/40). Pathogenic CNVs were found in 10 patients (25.0%), 15 CNVs of uncertain clinical significance were found in 12 patients (30.0%), and 7 likely benign CNVs were found in 4 patients (10.0%). CONCLUSION Chromosome CNVs play an important role in the pathogenesis of ID/DD/ASD. CNV-seq can detect chromosomal abnormalities including microdeletions and microduplications, which could provide a powerful tool for revealing the genetic etiology of ID/DD/ASD patients.
Pregnancy ; Child ; Male ; Humans ; Female ; DNA Copy Number Variations ; Intellectual Disability/genetics* ; Autism Spectrum Disorder/genetics* ; Developmental Disabilities/genetics* ; Abnormal Karyotype

Objective To investigate the clinical diagnostic value of serum procalcitonin(PCT),D-dimer (DD),C-reactive protein(CRP)in acute-on-chronic liver failure(ACLF). Methods 124 ACLF patients, 63 chronic hepatitis B patients,32 chronic hepatitis C patients,24 chronic hepatitis E patients and 60 healthy controls from the second affiliated hospital of Nanchang University were enrolled in this study.PCT was detected by a sandwish immunodetection method. D-dimer was detected by Latex Turbidimetry. CRP was detected by rate nephenometry. The detection results were used for analyzing the clinical diagnostic value of ACLF with infection. Results(1)The level of PCT,DD and CRP in ACLF group were significantly higher than non-ACLF group and healthy controls(P<0.05).The levels of PCT,DD and CRP in the infection group were significantly higher than non-infection group(P<0.05).(2)The positive rates of PCT,DD and CRP in the infection group were 93.24%, 78.38%,89.19%,which were significantly higher than the non-infection group and healthy controls respectively (P < 0.05).(3)The sensitivity(93.24%)and specificity(90.00%)of PCT were the highest among all indexes. (4)The area under the ROC curve of PCT,DD,CRP were 0.892,0.715,0.755,respectively.PCT had the highest diagnostic value. Conclusion The levels of serum PCT,DD and CRP have a significant clinical value for the early diagnosis of ACLF with infection.

Dissipative structure refers to a self-organized and orderly structure that exists far from equilibrium.The human body,considered a classical example,generates negative entropy through the exchange of matter,energy,and information with the environment to counteract the increase in entropy.In this paper,we organized theories and related research on dissipative structure and entropy,discussing their significance in regulating various aspects such as the human body,cancer,aging,and more.By selecting the special population of pregnant women,focusing on the information dimension,developing the corresponding information exchange scale(Cronbach's α>0.9),and proposing the information exchange index,we preliminarily explored the influence of the dissipative structure's information dimension on pregnancy health.The results showed a negative correlation between the information exchange index and anxiety scores during pregnancy(r =-0.35,P<0.001),with an OR value of 0.26(95%CI:0.08～0.80),preliminarily confirming the feasibility of conducting empirical research based on dissipative structure theory.If further relevant empirical studies are conducted,it is expected that new disease prevention strategies will be developed and new theories and methods will be provided for the field of public health.

AIM: To explore the possible mechanism of Radix Tetrastigma (RT) on anti-rheumatoid arthritis (RA). METHODS: The rat model of RA was established by intradermal injection of complete Freund]s adjuvant into the right hind foot of SD rats. RT Extract with different dosage was continuous intragastric administration for 3 weeks, then, the degree of foot swelling, arthritis index score, joint heat and grip of each rat was measured respectively. ELISA was used to detect the expression levels of Interleukin (IL) 6, IL-17, IL-10, tumor necrosis factor-α, and rheumatoid factor. Fully automatic hemorheometer was applied to measure hemorheology indexes. The number of CD4

Objective:To evaluate the efficacy and safety of chimeric antigen receptor T-cell (CAR-T) therapy followed by allogeneic hematopoietic stem cell transplantation (allo-HSCT) in patients with Ph-like acute lymphoblastic leukemia (Ph-ALL) .Methods:Patients with Ph-ALL who underwent CAR-T therapy followed by allo-HSCT from March 2018 to August 2023 at the First Affiliated Hospital of Soochow University were included, and their clinical data were retrospectively analyzed.Results:Of the 21 patients, 14 were male and 7 were female. The median age at the time of CAR-T therapy was 22 (6-50) years. Seven patients had ABL1-like rearrangements, and 14 had JAK-STAT rearrangements. Prior to CAR-T therapy, 12 patients experienced hematologic relapse; 7 were multiparameter flow cytometry minimal residual disease (MFC-MRD) -positive and 2 were MFC-MRD-negative. CAR-T cells were derived from patients’ autologous lymphocytes. Nine patients were treated with CD19 CAR-T cells, and 12 were treated with CD19/CD22 CAR-T cells. After assessment on day 28 after CAR-T therapy, 95.2% of the patients achieved complete remission, with an MRD-negative remission rate of 75%. Nineteen patients developed grade 0–2 cytokine release syndrome (CRS) and 2 patients suffered grade 3 CRS, all cases of which resolved after treatment. All patients underwent allo-HSCT after CAR-T therapy. The median time from CAR-T therapy to allo-HSCT was 63 (38-114) days. Five patients experienced relapse after CAR-T therapy, including four with hematologic relapse and one with molecular relapse. The 3-year overall survival (OS) rates in the ABL1 and JAK-STAT groups were (83.3±15.2) % and (66.6±17.2) %, respectively ( P=0.68) . The 3-year relapse-free survival (RFS) rates were (50.0±20.4) % and (55.6±15.4) % in the ABL1 and JAK-STAT groups, respectively. There was no significant difference in 3-year OS or RFS between the two groups. Conclusions:CAR-T therapy followed by allo-HSCT leads to rapid remission in most patients with Ph-ALL and prolongs leukemia-free survival.