Objective To systematically review the correlation between polymorphisms of CYP2D6 genotypes and concentrations of tamoxifen and its activity in Chinese breast cancer patients.Methods Such databases as Cochrane Library, PubMed, EMBase, CNKI, CBM, WeipuData and WanfangDate,from January 1995 to October 2014 were searched on line for the studies about the correlation between polymorphisms of CYP2D6 genotypes concentrations of tamoxifen and its activity in Chinese breast cancer patients.And references about it were checked.The meta-analysis was performed with RevMan 5.3 software.Results A total of 4 articles involving 438 patients were included.The results of meta-analysis showed that,the concentrations of HTAM and endoxifen in patients who had CYP2D6*10/*10 genotypes were lower than the other genotypes ( P<0.0001 ) .The concentration of tamoxifen in patients who had CYP2D6*10/*10 genotypes was lower than CYP2D6Wt/Wt ( P<0.05 ) .There was no significant difference between CYP2D6Wt/Wt and CYP2D6Wt/*10.Conclusion We concluded that CYP2D6 polymorphisms may influence concentrations of tamoxifen and its activity in Chinese breast cancer patients.

Objective To investigate the expression and clinical significance of LINC00638 in colon cancer,and further to explore the impact of LINC00638 on the biological function of colon cancer cells.Methods The cancer genome atlas(TCGA)database was used to preliminarily compare the expression differences of LINC00638 in normal colon tissue and colon cancer tissue,and to explore the rela-tionship between LINC00638 and clinical pathological characteristics of patients.Kaplan-Meier survival analysis was used to further in-vestigate the effect of LINC00638 expression on the prognosis of colon cancer patients.The GEPIA database and the Gene Expression Om-nibus(GEO)database were used to further validate the effect of LINC00638 expression on the prognosis of colon cancer patients.Univari-ate and multivariate COX regression analysis were used to explore the effect of LINC00638,clinical and pathological related factors on the prognosis of colon cancer patients.Small interfering RNA(siRNA)was used to silence the expression of LINC00638 in HCT116 cells and DLD1 cells,and the effect of LINC00638 on the proliferation,migration and invasion of colon cancer cell was explored through CCK-8 experiment,colony formation experiment,scratch experiment and Transwell experiment.Results The expression level of LINC00638 was significantly increased in colon cancer tissue,and its expression level was closely related to lymph node metastasis and TNM staging in co-lon cancer patients.The results of Kaplan-Meier survival curve analysis showed that the higher the expression level of LINC00638,the worse the prognosis of colon cancer patients(P＜0.05).The results of COX regression analysis showed that LINC00638,age,and TNM staging could be independent prognostic factors for colon cancer patients.The results of cell function experiments showed that compared with the control group,the proliferation,migration,and invasion ability of cells in knockdown group were significantly decreased(P＜0.05).Conclusion LINC00638 is highly expressed in colon cancer,and the higher its expression level,the worse the prognosis of colon cancer patients.After knocking down LINC00638,it can inhibit the proliferation,migration and invasion ability of colon cancer cells.