1.Isolation and identification of Vibrio harveyi from chub mackerel (Scomber japonicus).
Young Ran LEE ; Jin Woo JUN ; Sib Sankar GIRI ; Hyoun Joong KIM ; Sae Kil YUN ; Cheng CHI ; Sang Guen KIM ; Jeong Rack KOH ; Ji Yun JUNG ; Se Chang PARK
Korean Journal of Veterinary Research 2016;56(1):45-46
For several days, there was a series of mortalities of chub mackerel (Scomber japonicus) that were reared for public exhibition in a private aquarium in Seoul, Korea. As part of the diagnosis of the dead fish, a bacterial isolate from the kidney was cultured, identified, and confirmed to be Vibrio (V.) harveyi using Vitek System 2 and 16S rRNA gene sequencing. Phylogenetic analysis was also performed by the neighbor-joining method. As a result, the V. harveyi isolated from chub mackerels of a private aquarium in Korea, called as SNUVh-LW1, was clustered in the same group with V. harveyi ATCC33843.
Cyprinidae*
;
Diagnosis
;
Genes, rRNA
;
Kidney
;
Korea
;
Mortality
;
Perciformes*
;
Seoul
;
Vibrio*
2.Isolation and molecular identification of Vibrio harveyi from the look down fish (Selene vomer).
Jin Woo JUN ; Young Ran LEE ; Sib Sankar GIRI ; Hyoun Joong KIM ; Sae Kil YUN ; Cheng CHI ; Sang Guen KIM ; Jeong Rack KOH ; Ji Yun JUNG ; Byeong Chun LEE ; Se Chang PARK
Korean Journal of Veterinary Research 2016;56(2):125-127
Gradual mortality of look down fish (Selene vomer) was observed in a private aquarium in Seoul, showing abnormal swimming behavior and lethargy. A bacterial pathogen from kidney was cultured, identified, and confirmed as Vibrio harveyi using Vitek System 2 and 16S rRNA gene sequencing. A predominant bacterial strain, SNUVh-LW2 was proved to be most closely related to isolates from China by phylogenetic analysis with minimum evolution method. Also, tetracycline was considered as the most sensitive antibiotic agent via antibiotic usceptibility test. The group of fish was treated according to the diagnostic result and no more mortality was observed.
China
;
Genes, rRNA
;
Kidney
;
Lethargy
;
Methods
;
Mortality
;
Seoul
;
Swimming
;
Tetracycline
;
Vibrio*
3.Isolation of a zoonotic pathogen Aeromonas hydrophila from freshwater stingray (Potamotrygon motoro) kept in a Korean aquarium with ricefish (Oryzias latipes).
Saekil YUN ; Young Ran LEE ; Sib Sankar GIRI ; Hyoun Joong KIM ; Cheng CHI ; Sang Guen KIM ; Sang Wha KIM ; Jin Woo JUN ; Se Chang PARK
Korean Journal of Veterinary Research 2017;57(1):67-69
In the present study, Aeromonas (A.) hydrophila was isolated from a captive-bred adult freshwater stingray (Potamotrygon motoro) reared at a commercial aquarium in Korea. The stingray had bites on its fins, hemorrhages on the ventral part, and congested internal organs. A bacterium was isolated from kidney and subsequently identified as A. hydrophila. Based on phylogenetic analysis results, the isolate in the present study (SNUAh-LA1) was most closely related to A. hydrophila AH10 (China) and A. hydrophila AKR1 (Korea). It is most likely that the pathogen infection resulted from Potamotrygon motoro cohabiting with ricefish (Oryzias latipes).
Adult
;
Aeromonas hydrophila*
;
Aeromonas*
;
Estrogens, Conjugated (USP)
;
Fresh Water*
;
Hemorrhage
;
Humans
;
Kidney
;
Korea