1.Review of Pathogenic Nocardias in Cultured Fish
Si-Ping YUAN ; Guo-Liang WANG ; Shan JIN ;
Microbiology 1992;0(02):-
Some studies on nocardiosis in cultured fish were critically reviewed,including pathogenic bacterium,taxonomy,pathogenicity,histopathologically,diagnosis,controlling method and immunology. Some problem and advice on this disease were suggested.
2.Effects of hydrogen peroxide on cyclooxygenase-2 expression in pulmonary arterial endothelial cells and the role of CaMKⅡ
Liping ZHU ; Si JIN ; Yuan SU ; Dixun WANG
Chinese Journal of Pathophysiology 2000;0(10):-
AIM: To investigate if hydrogen peroxide may alter COX-2 gene expression in pulmonary artery endothelial cells(PAECs) and how CaMKⅡ functions in this process.METHODS: Cultured pulmonary arterial endothelial cells were treated with different concentrations of hydrogen peroxide for different durations.The cells survival rates were measured by CCK-8 after the cells were treated by hydrogen peroxide.The level of COX-2 mRNA and protein were measured by RT-PCR and Western blotting,respectively.RESULTS: The results showed that hydrogen peroxide up-regulated COX-2 mRNA and protein levels in a concentration-and time-dependent manners.Incubation with 100 ?mol/L H2O2 for 4 h increased COX-2 mRNA and protein level to 256.01%?22.36%(P
3.Effect of ABA on transcriptionally active Ty1-copia retrotransposons in Dendrobium officinale.
Cong LI ; Jin-Ping SI ; Yan-Hui GAO ; Yu-Qiu ZHU ; Yuan JIANG
China Journal of Chinese Materia Medica 2014;39(10):1788-1794
Using universal primer Tyl-copia retrotransposon RT, the conserved reverse transcriptase domain of about 260 bp was amplified by RT-PCR from the Dendrobium officinale which induced by 100 micromol x L(-1) abscisic acid (ABA), indicating these retrotransposons activated by 100 micromol x L(-1) ABA. The amplicons were recovered and cloned,then sequenced and analyzed by related bioinformatics software. Forty-two Ty1-copia like retrotransposon RT transcriptionally activated were obtained with high heterogeneity. The length of these sequences varied from 247 to 266 bp, and was rich in AT and homology ranged from 46.3% to 98.9%. The same to Ty1-copia like retrotransposon RT of genome, different c/s-acting regulatory elements induced by stress conditions and the starting transcription signals, corresponding to CAAT box, TATA box conserved sequences and some other regulatory elements. The c/s-acting regulatory elements induced by stress conditions of reverse transcriptase transcriptionally activated of Tyl-copia retrotransposons were significantly increased than that of Ty1-copia like retrotransposon RT of genome. When being translated into amino acids, fifteen sequences presented stop codon mutation, nineteen sequences presented frameshift mutation, and all sequences presented conserved sequence "SLYGKQ" mutation. Five categories were identified through phylogenic analysis after alignment analyses of their amino acid sequences, and with Ty1-copia like retrotransposon RT of genome having low homology, which indicated that reverse transcriptase transcriptionally activated of Ty1-copia retrotransposons which induced by ABA had Significantly differences with Ty1-copia like retrotransposon RT of genome.
Abscisic Acid
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pharmacology
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Amino Acid Sequence
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Dendrobium
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drug effects
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genetics
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Molecular Sequence Data
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Phylogeny
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Retroelements
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drug effects
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Sequence Homology, Amino Acid
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Transcription, Genetic
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drug effects
4.The expression of basic fibroblast growth factor 1 during human embryonic yolk sac hematopoiesis.
Yue-Si WANG ; Jian-Yuan LI ; Shao-Hua JIN ; Hai-Yan WANG
Chinese Journal of Hematology 2008;29(8):535-539
OBJECTIVETo explore the effect of basic fibroblast growth factor 1 (bFGF1) during embryonic development on hematopoiesis, to study the expression of FGF1, vascular endothelial growth factor receptor (KDR), CD133, CD34 and transcription factors Ihh, SCL, GATA-1, GATA-2 and PU. 1 in the yolk sac, and to learn about the role and relationships of FGF1, hematopoietic cells and transcription factors during embryonic hematopoiesis.
METHODS10 microm sections and total RNA were prepared from 107 human embryos aged 3-12 weeks. Immunohischemical SP staining and RT-PCR were performed.
RESULTSThe yolk sac blood islands of human 3 approximately 12 weeks embryos consisted of peripheral vascular endothelial cells and central hematopoietic cells. The expression of FGF1 was firstly found in visceral mesoderm around periphery of yolk sac blood island at day 16, while was little inside it. KDR was not or lowly expressed and CD34 and CD133 were not expressed then. The expression increased, gray value decreased and staining enhanced at day 21. Strong staining of CD34+, CD133+ and KDR+ cells were found in blood island and mesoderm at day 30, their gray values changed from 156 +/- 16, 173 +/- 18 and 160 +/- 14 to 53 +/- 7, 52 +/- 6 and 69 +/- 8 respectively. FGF1 expression was strong positive, the gray value declined dramatically from 161 +/- 13 to 40 +/- 5. Some positive cells formed vessel-like structure along the periphery of blood island. Moderate expression of CD34+, CD133+, KDR+ cells increased at day 45, the cells aggregated into mass in blood island and FGF1+ cells did the same in blood island, while little in mesoderm. Its gray valve was increased. After 7 weeks, CD133+, KDR+, CD34+ cells significantly decreased their gray values increased, the staining became week. FGF1 was weakly expressed in yolk sac and its gray value increased to 179 +/- 22. RT-PCR showed Ihh, SCL, GATA-1 and GATA-2 were expressed at different time in yolk sac. PU. 1 were not expressed at day 16, and then expressed.
CONCLUSIONSThe hematopoietic properties of yolk sac may be dependent on signaling through FGF receptors and FGF1 plays an important role in hematopoietic stem cell homeostasis. The FGF pathway regulates primitive hematopoiesis by modulating transcription factors such as Gata1 expression level and activity.
Embryo, Mammalian ; metabolism ; physiology ; Fibroblast Growth Factor 1 ; metabolism ; Hematopoiesis ; Humans ; In Vitro Techniques ; Yolk Sac ; metabolism ; physiology
5.Analysis of application effect of orthopedic medical device intelligent acceptance equipment combined with UDI code in the management of orthopedic medical devices
Xiangjun WANG ; Hong YUAN ; Qin WANG ; Weiling HU ; Jiangtao SI ; Si SHEN ; Jiao JIN
China Medical Equipment 2024;21(5):166-170
Objective:To analyze the application effect of intelligent acceptance equipment for orthopedic medical equipment combined with medical device unique device identifier(UDI)code in the management of orthopedic medical devices.Methods:Based on the UDI code,the UDI application process of orthopedic medical devices was formulated,and all operations such as inspection and accurate billing of orthopedic medical devices were completed through the identification and comparison of intelligent acceptance equipment for orthopedic medical devices.A total of 8 317 orthopedic instruments used in orthopedic surgery in Wangjing Hospital of China Academy of Chinese Medical Sciences from September to December 2023 were selected.According to the different time of the implementation of intelligent acceptance equipment for orthopedic medical devices,the 4 072 orthopedic devices used from September to October 2023 was marked as before implementation and adopted the traditional management mode,the 4 245 orthopedic devices used from November 2023 to December 2023 were marked as before implementation and adopted the intelligent acceptance management mode.The management effect and incidence of adverse events were compared before and after the implementation of intelligent acceptance equipment for orthopedic medical devices.Results:The total effective rate of management after the implementation of intelligent acceptance equipment for orthopedic medical instruments was 98.78%,which was significantly higher than that before the implementation,the difference was statistically significant(x2=272.03,P<0.01).The total incidence of adverse events after the implementation of the intelligent acceptance equipment for orthopedic medical devices was 8.83%,which was significantly lower than that before the implementation,the difference was statistically significant(x2=281.38,P<0.01).Conclusion:The intelligent acceptance device combined with UDI code for orthopedic medical devices can achieve efficient distribution,accurate acceptance and billing,reduce the occurrence of adverse events,meet the requirements of the whole process traceability management of one thing and one code,and improve the management level orthopedic medical devices.
6.Microsurgery for intracranial aneurysm:analysis of 438 cases.
Shi-yuan ZHANG ; Chao YOU ; Jin-ping LIU ; Bo-yong MAO ; Min HE
Chinese Journal of Surgery 2008;46(8):598-601
OBJECTIVETo summarize the experience of surgical therapy in a series of 438 patients with intracranial aneurysms.
METHODSA retrospective analysis was made on the clinical data of 438 patients, in terms of the perioperative management, timing of surgery, surgical skills, and The HUNT-HESS grade.
RESULTS438 patients with 476 aneurysms underwent microsurgery, in which 450 aneurysms were clipped, 14 were wrapped, 8 were isolated,4 were cut. 32 aneurysms were ruptured (6.72%) during the operation. The relation between Hunt-Hess scale and mortality is significant postpone operation (> or =7 d) has got a better curative effect than the early.
CONCLUSIONSMicrosurgical treatment is a confirmed effective method with intracranial aneurysms. The higher of HUNT-HESS grade will result to the worse clinical outcome.
Adolescent ; Adult ; Aged ; Child ; Female ; Humans ; Intracranial Aneurysm ; mortality ; surgery ; Male ; Microsurgery ; methods ; Middle Aged ; Neurosurgical Procedures ; methods ; Prognosis ; Retrospective Studies ; Treatment Outcome
7.Characteristics of drug-release in vitro of different dextran-dexamethasone conjugates.
Si-yuan ZHOU ; Qi-bing MEI ; Li LIU ; Bang-le ZHANG ; Chen LI ; Jin ZHOU
Acta Pharmaceutica Sinica 2003;38(5):388-391
AIMTo evaluate the effects of molecular weight of dextran on drug-release of conjugate in vitro by screening colon-specific conjugates.
METHODSThe conjugates, synthesized with different molecular-weight dextran and dexamethasone, were incubated in the contents of different parts of rat gastrointestinal tract at 37 degrees C. The release of dexamethasone(Dex) and dexamethasonehemisuccinate was determined by HPLC. The mobile phase consisted of 35% acetonitrile and 65% trisodium citrate (50 mmol.L-1, adjusted to pH 4.1 with phosphoric acid).
RESULTSThere was no release of dexamethasone or dexamethasonehemisuccinate from conjugates in the stomach contents. The amount of Dex (including dexamethasonehemisuccinate) released from DexD26 in the contents of colon and cecum was shown to be 4.0 times higher than that released in the contents of proximal and distal small intestine while the amount of Dex (including dexamethasonehemisuccinate) released from DexD50 was shown to be 3.6 times higher. The amount of Dex (including dexamethasonehemisuccinate) released from DexD2 in the contents of colon and cecum and from DexD7.6 were 2.0 times and 1.9 times higher, respectively, than that released in contents of proximal and distal small intestine.
CONCLUSIONThe molecular weight of dextran showed marked effect on drug-release of the conjugate in vitro, and the conjugates with larger molecular-weight dextran have great potential in colon-specific delivery of dexamethasone.
Animals ; Colon ; metabolism ; Dexamethasone ; administration & dosage ; metabolism ; Dextrans ; chemistry ; Drug Carriers ; Drug Compounding ; Drug Delivery Systems ; Female ; In Vitro Techniques ; Intestine, Small ; metabolism ; Male ; Molecular Weight ; Rats ; Rats, Sprague-Dawley ; Stomach ; metabolism
8.A case-crossover study on road traffic injury.
Ping YUAN ; Jin WEN ; Zhen-hua DENG ; Bin KONG ; Kuan-lin LIU ; Si-xing HUANG
Chinese Journal of Epidemiology 2005;26(8):600-603
OBJECTIVETo explore the risk factors of road traffic injury (RTI), and to provide evidence for stretagy development on the prevention and control of RTI.
METHODSCase-crossover study was used. 402 drivers were face to face interviewed by specially designed questionnaire including the items as drinking alcohol before driving, onset anger scale (OAS), anxiety/irritation, Stanford sleepiness scale (SSS), vehicle breakdown, carelessness, violating the rules on right of way and traffic signs when driving. Tables related to case-control study on 1:1 matching was used for single factor analysis, and conditional logistic regression was used for multifactor analysis.
RESULTSThe risk factors that were associated with RTI including high OAS (OAS = 5-7, OR = 114.88, 95% CI:4.29-3074.86), violating the rules on right of way (OR = 46.96, 95% CI:12.74-173.11), violating traffic signs when driving(OR = 24.57, 95% CI: 8.69-69.45), carelessness (OR = 14. 54,95% CI: 6.85-30.86), unsafe distance between vehicles( OR = 14.30,95% CI: 3.68-55.60), wrong judgement (OR = 14.67,95% CI:4.88-44.10), vehicle breakdown (OR = 8.57, 95% CI: 1.38- 53.27) and improper measure ( OR = 8.46, 95% CI: 2.46-29.14) etc.
CONCLUSIONImproper driving behaviors, abnormal psychological status and driving skills of drivers were related to RTI, suggesting that it was necessary to carry out continous traffic security education to the drivers.
Accidents, Traffic ; statistics & numerical data ; Adult ; Cross-Over Studies ; Demography ; Female ; Humans ; Logistic Models ; Male ; Multivariate Analysis ; Risk Factors ; Surveys and Questionnaires ; Wounds and Injuries ; epidemiology ; etiology ; prevention & control
9.HPV infection and its correlation with survivin and PCNA expression in condyloma acuminatum
Yun LIN ; Ya-Ting TU ; Shah-Juan CHEN ; Si-Yuan CHEN ; Li-Xia ZHANG ; Yan WU ; Juan TAO ; Jin-bo CHEN ;
Chinese Journal of Dermatology 1994;0(05):-
Objective To investigate HPV infection and its correlation with the expression of sur- vivin and proliferating cell nuclear antigen (PCNA) in condyloma acuminatum (CA).Methods HPV6/11 and 16/18 DNA were detected by PCR in 41 cases of CA and 23 specimens of normal skin.The expression of survivin and PCNA was measured by immunohistochemical technique in these two groups. Results The infection rates of HPV6/11 and 16/18 were 87.80% (36/41) and 19.51%(8/41),respective- ly,in CA,and 0 in normal skin epidermis.The expression of survivin and PCNA was found in 53.66% (22/41) and 87.80% (36/41) of CA ,and was 8.70%(2/23)and 47.83% (11/23) in normal skin,respec- tively.The expression of survivin was positively correlated with the high expression of PCNA in CA.The expression of survivin and PCNA was higher in HPV6/11 positive lesions than in HPV6/11 negative lesions. Conclusion HPV infection may up-regulate the expressions of survivin and PCNA,inhibit the apoptosis and promote the proliferation of keratinocytes.
10.Knowledge discovery based on artificial neural network data mining in population pharmacokinetics
Hai-Yan WANG ; Lu-Yi ZHOU ; Si-Bo LU ; Li YANG ; Jin-Yuan HUANG ; Cheng-Yu LU
The Chinese Journal of Clinical Pharmacology 2018;34(20):2449-2451
Objective Artificial neural networks ( ANN) is an integral part of artificial intelligence.In this paper, the architecture of artificial neural networks in population pharmacokinetics is discussed and summa -rized according to the data mining techniques of medical information . Methods The data samples of population pharmacokinetics in network databases are collected by data mining , and a large sample population pharmacokinetics ( PPK) database is generated by simulation , and an artificial neural network PPK model based on SPSS system is established . Results The simulated large sample PPK data have the same statistical characteristics as those reported in the literature.The ANN model is sim-ple and the analysis accuracy is better. Conclusion ANN analysis model can be reconstructed by PKK data mining and simulation .