3.Alpha-fetoprotein-high-producing hepatoid adenocarcinoma of the stomach:A case report and litera-ture review
Wei MAO ; Qingjuan CHEN ; Xiaomin SI ; Xiao ZHANG ; Cheng JIANG
Practical Oncology Journal 2017;31(1):39-42
Hepatoid adenocarcinoma of the stomach ( HAS) belongs to one of the rare cases in gastric cancer types ,which has extremely high malignant degree and poor prognosis .Lymph node metastasis and liver me-tastasis are common in HAS.In this article,we reported alpha-fetoprotein-high-producing hepatoid adenocar-cinoma of the stomach(HAS),and reviewed the related literature at home and abroad in order to improve clinical physicians understanding of these diseases and treatment experience .
4.Simultaneous Determination of Asarinin, Prim-O-glucosylcimifugin and 5-O-Methylvisammioside in Xinqin Granules by HPLC
Xiaolong ZHANG ; Xiao SUN ; Si SUN ; Zhe LI
China Pharmacist 2014;(12):2027-2029
Objective:To simultaneously determine the contents of asarinin, prim-O-glucosylcimifugin and 5-O-methylvisammio-side in Xinqin granules. Methods:An HPLC method was used. The determination was performed on a ZORBAX Eclipse XDB-C18 col-umn(150 mm ×4.6mm,5 μm) with the mobile phase consisting of menthol (A)-water (B) with gradient elution. The flow rate was 1. 0 ml·min-1 . The column temperature was 30℃. The detection wavelength was set at 254 nm from 0 to 30 min and 287nm from 30 to 55 min. The injection volume was 10μl. Results:The linear range of prim-O-glucosylcimifugin, asarinin and 5-O-methylvisammio-side was 10.210-163.400 μg·ml-1(r=0.999 7),10.160-162.600 μg·ml-1(r=0.999 8) and 5.015-80.240 μg·ml-1(r=0. 999 8), respectively. The average recovery was 100. 30%(RSD=1. 6%, n=6),101. 53%(RSD=1. 1%,n=6) and 101. 12%(RSD=1. 2%, n=6), respectively. Conclusion: The method is simple and accurate, which can be used in the quality control of Xinqin granules.
5.Time-effect Relationship of Acupuncture in Improving Cardiac Vegetative Nerve Function in Patients with Type Ⅱ DM
Youmei CHEN ; Hui SI ; Yaxi ZHANG ; Lihong KONG ; Yuanchun XIAO
Journal of Acupuncture and Tuina Science 2004;2(6):27-28
Forty cases of type Ⅱ diabetes mellitus were treated by puncturing point Neiguan (PC 6), and the effect on their cardiac vegetative nerve functions were observed at 20 min, 40 min and 60 min after acupuncture respectively. The findings showed that all heart rate variables improved remarkably at the three time periods after acupuncture treatment, with significant differences (P<0.01); but there was no significant difference in the curative effects among the three time periods.
6.THE ISOLATION,SCREENING AND IDENTIFICATION OF BACILLUS SUBTILIS STRAINS PRODUCING HIGH-ACTIVITY FIBRONOLYSIN
Si-Yu LIANG ; Zhao-Xin LU ; Xiao-Kui ZHOU ; Xiao-Dong ZHANG ;
Microbiology 1992;0(05):-
The results of isolation,screening and identification of Bacillus subtilis strains which produced fibronolysion were reported in this paper.20 Bacillus subtilis strains were screened from different withered straw samples.Among them,FM-S1,FM-S2,FM-S6,FM-S8 and FM-S11 produce fibronolysin with higher activity.According to morphological,physiological and biochemical characters,the screened strains were confirmed to be Bacillus subtilis. For FM-S2,the production of fibronolysin type in solid fermentation was considered to be synchronous with growth.
7.Diagnosis of synovial sarcoma by fine-needle aspiration biopsy and interphase FISH: report of a case.
Sheng-jun XIAO ; Xiao-ling ZHANG ; Ming-qiang ZENG ; Si-en ZENG ; Hong-tao YE
Chinese Journal of Pathology 2010;39(4):278-279
Adolescent
;
Biopsy, Fine-Needle
;
Chromosomes, Human, Pair 18
;
Female
;
Humans
;
In Situ Hybridization, Fluorescence
;
Oncogene Proteins, Fusion
;
genetics
;
Proto-Oncogene Proteins c-bcl-2
;
metabolism
;
Sarcoma, Synovial
;
genetics
;
metabolism
;
pathology
;
Soft Tissue Neoplasms
;
genetics
;
metabolism
;
pathology
;
Translocation, Genetic
;
Vimentin
;
metabolism
8.C825T polymorphism of G protein beta3 subunit gene and Uygur Hilit type of essential hypertension: a correlation study.
Xiao-Xi LI ; Yu-Nu-Si A-YI-GU-LI ; Jing-Jing HUANG ; Jing-Ping ZHANG ; A-Xi-Mu-Jiang KA-SI-MU-JIANG ; Yu-Nu-Si KU-RE-XI
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(3):297-302
OBJECTIVETo explore the association between C825T polymorphism of G protein beta3 subunit (GNB3) gene and different Hilit types of essential hypertension (EH) in the Uygur nationality of Xinjiang.
METHODSAccording to Uygur medical theories, EH patients (as the EH group) and non-EH patients (as the control group) were assigned to four Hilit groups. The C825T polymorphism of GNB3 was detected in 161 EH patients and 379 non-EH subjects of different Hilit types by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to explore the difference of the genotypes and allelic frequencies and hypertension.
RESULTS(1) In Xinjiang Uygur population, the distribution frequencies of GNB3 C825T polymorphism were in accordance with Hardy-Weinberg (chi2 = 0.871, P = 0.647). (2) There was no statistical difference in the distribution frequencies of three genotypes and two alleles of GNB3 between the EH group and the control group (P > 0.05). (3) There was statistical difference in distribution frequencies of three genotypes between the abnormal Sapra and non-abnormal Sapra group (the sum of abnormal Sewda, abnormal Kan, and abnormal Balhem) (chi2 = 6.905, P = 0.032), especially between the abnormal Sapra and abnormal Balhem groups (chi2 = 10.404, P = 0.006), but there was no statistical difference in distribution frequencies of alleles between the two groups (P > 0.05). (4) In 161 EH patients, there was statistical difference in the distribution frequencies of three genotypes and two alleles between the abnormal Sapra and non-abnormal Sapra group (chi2 = 9.034, P = 0.011; chi2 = 4.701, P = 0.03).
CONCLUSIONSBoth TT genotype and T allele of GNB3 C825T polymorphism might not be associated with EH patients in Xinjiang Uygur populations. However, they were correlated with hypertension patients of non-abnormal Sapra, indicating the pathogeneses of EH with different Hilit types might be different.
Adult ; Aged ; Alleles ; Case-Control Studies ; Essential Hypertension ; Female ; Gene Frequency ; Genotype ; Heterotrimeric GTP-Binding Proteins ; genetics ; Humans ; Hypertension ; classification ; diagnosis ; genetics ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Minority Groups ; Polymorphism, Genetic
9.DAZ1/DAZ2 cluster deletion mediated by gr/gr recombination per se may not be sufficient for spermatogenesis impairment: a study of Chinese normozoospermic men.
Yuan YANG ; Cui-Ying XIAO ; Zhou-Cun A ; Si-Zhong ZHANG ; Xiang LI ; Si-Xiao ZHANG
Asian Journal of Andrology 2006;8(2):183-187
AIMTo explore the possible effect of the deleted in azoospermia (DAZ) copy cluster deletion on spermatogenesis in the Chinese population, the deletion of the azoospermia factor c (AZFc) region was analyzed in 346 normozoospermic men.
METHODSThree DAZ single nucleotide variant loci and seven AZFc-specific sequence-tagged sites were examined with polymerase chain reaction (PCR)-restriction fragment length polymorphism and routine PCR.
RESULTSFive (1.4%) of the normozoospermic men were found to have deletion of gr/gr-DAZ1/DAZ2. None of the men were found to have b2/b4-entire DAZ deletion.
CONCLUSIONThe presence of gr/gr-DAZ1/DAZ2 deletion in five men with normozoospermia suggests that this deletion per se may not be sufficient for spermatogenic impairment in Chinese men.
Adult ; Asian Continental Ancestry Group ; genetics ; China ; Chromosomes, Human, Y ; genetics ; Gene Deletion ; Humans ; Male ; Oligospermia ; genetics ; Polymorphism, Restriction Fragment Length ; RNA-Binding Proteins ; genetics ; Recombination, Genetic ; genetics ; Sequence Tagged Sites ; Spermatogenesis ; genetics