2.Gastric fistulation with transcutaneous endoscopy in a child.
Zhi-hong HU ; Ming SHEN ; Li SUN ; Rong QIAO ; Fu-mei JIA ; Si-yuan YANG
Chinese Journal of Pediatrics 2004;42(3):222-223
3.Circulatory sleep apnea: Preliminary report of clinical observation on sleep apnea in patients with chronic heart failure.
Si-xin XIE ; Xing-guo SUN ; Fu-rong WANG ; Xiao-yue TAN ; Xue-mei ZHANG
Chinese Journal of Applied Physiology 2015;31(4):329-331
OBJECTIVEThe aim of this study is to investigate the occurrence and mechanism of Cheyne-Stokes breathing pattern in patients with heart failure.
METHODSFifty-six patients who performed polusomnography sleep testing at National Center of Cardiovascular Diseases Fuwai Hospital from March to May in 2015. We divided them into chronic heart failure (CHF) group and non-CHF group.
RESULTSThe occurrences of sleep apnea in two groups were high. In CHF group (n = 11) , there were 10 patients with apnea hypopnea index (AHI) > 5; and their AHI was 23.93 ±14.63. In non-CHF group (n = 45), there were 33 patients whose AHI > 5; and their AHI was 16.20 ± 18.76. The ratio of center sleep apnea to all gross sleep apnea ratio in CHF group was higher than that in non-CHF group (80.21% ± 30.55% vs 27.16% ± 35.71%, P < 0.01 ).
CONCLUSIONBased upon the new theory of holistic integrative physiology and medicine, we explain the mechanism of circulatory dysfunction induce the oscillation breathing in patients with CHF. The sleep apnea and C-S respiration in CHF should be called circulatory sleep apnea, rather than central sleep apnea.
Cheyne-Stokes Respiration ; Chronic Disease ; Heart Failure ; physiopathology ; Humans ; Polysomnography ; Sleep Apnea Syndromes ; physiopathology ; Sleep Apnea, Central
4.Drug-free targeted thrombolytic strategy based on gold nanoparticles-loaded human serum albumin fusion protein delivery system
Jin-jin LU ; Chun LIU ; Si-rong SUN ; Jing-hua CHEN ; Min GAO
Acta Pharmaceutica Sinica 2024;59(2):455-463
Thrombus is a major factor leading to cardiovascular diseases such as myocardial infarction and stroke. Although fibrinolytic anti-thrombotic drugs have been widely used in clinical practice, they are still limited by narrow therapeutic windows, short half-lives, susceptibility to inactivation, and abnormal bleeding caused by non-targeting. Therefore, it is crucial to effectively deliver thrombolytic agents to the site of thrombus with minimal adverse effects. Based on the long blood circulation and excellent drug-loading properties of human serum albumin (HSA), we employed genetic engineering techniques to insert a functional peptide (P-selectin binding peptide, PBP) which can target the thrombus site to the
5.Structure modification and antimicrobial activity of novel cationic melittin analogues
A-long CUI ; He-xian YANG ; Si-tu XUE ; Lian-qi SUN ; Jie JIN ; Hong YI ; Zhuo-rong LI
Acta Pharmaceutica Sinica 2021;56(5):1424-1428
Melittin exhibits high antibacterial potency against drug-resistant bacteria. However, the clinical utility of melittin is limited by its serious hemolytic activity. Thus, the need for developing novel melittin analogues with high antimicrobial activity and low hemolytic activity has grown. We designed, synthesized, and evaluated 20 novel melittin analogues with varying hydrophobic, polar or positively charged amino acids. The results showed that 8 compounds had antimicrobial activity (MIC: 1-4 μg·mL-1) against gram-positive pathogens equal to or better than that of melittin, and 16 compounds had low hemolytic activity (HC50 ≥ 11.9 μg·mL-1). Compounds
6.Involvement of MAPK pathways in NMDA-induced apoptosis of rat cortical neurons.
Xiao-Rong YANG ; Ping SUN ; Hua-Ping QIN ; Pei-Pei SI ; Xue-Fei SUN ; Ce ZHANG
Acta Physiologica Sinica 2012;64(6):609-616
NMDA-induced excitotoxicity cause severe neuronal damage including apoptosis and necrosis. The present study was aimed to evaluate the proportion of NMDA-induced apoptosis of rat cortical neurons and discover signal transduction mechanism. Caspase inhibitor and lactate dehydrogenase (LDH) assay were used to study the NMDA-induced apoptosis. To explore the involved signal pathways, the primary culture of rat cortical neurons were pretreated by the inhibitors of three MAPK pathways, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 MAPK. With 2 h of NMDA treatment, cellular apoptosis was measured by caspase-3 activity, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) and Annexin V staining. The results showed that: (1) Caspase-dependent apoptosis accounted for 22.49% in NMDA-induced neuronal death; (2) Pretreatment with p38 MAPK inhibitor SB203580 (10 μmol/L) significantly decreased NMDA-mediated caspase-3 activity by 30.43% (P < 0.05). However, ERK inhibitor PD98059 (20 μmol/L) or JNK inhibitor SP600125 (20 μmol/L) did not influence caspase-3 activity; (3) Pretreatment with SB203580 significantly reduced the number of NMDA-induced TUNEL-positive cells by 33.10% (P < 0.05). PD98059 (20 μmol/L) or SP600125 (20 μmol/L) did not show obvious effect; (4) Pretreatment with SB203580 (10 μmol/L) significantly reduced the number of NMDA-induced early apoptotic neurons by 55.56% (P < 0.05). Also, SP600125 (20 μmol/L) significantly decreased the amount of late apoptotic/dead cells by 67.59% (P < 0.05). There was no effect of PD98059 (20 μmol/L). These results indicate that: (1) NMDA induces neuronal apoptosis besides necrosis; (2) p38 MAPK, but not JNK and ERK, is involved in NMDA-induced neuronal apoptosis, and inhibition of the apoptotic signaling pathway contributes to neuroprotection; (3) JNK activation might contribute to NMDA-induced neuronal necrosis rather than apoptosis.
Animals
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Anthracenes
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pharmacology
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Apoptosis
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Caspase 3
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metabolism
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Cells, Cultured
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Extracellular Signal-Regulated MAP Kinases
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antagonists & inhibitors
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Imidazoles
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pharmacology
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JNK Mitogen-Activated Protein Kinases
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antagonists & inhibitors
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MAP Kinase Signaling System
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N-Methylaspartate
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pharmacology
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Neurons
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cytology
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Primary Cell Culture
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Pyridines
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pharmacology
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Rats
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p38 Mitogen-Activated Protein Kinases
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antagonists & inhibitors
7.Application of percutaneous needle aspiration biopsy by the simulator guided to the diagnosis for pulmonary focus in coal miners' pneumoconiosis.
Si-hai LIU ; Cheng-dong QI ; Wen-shou XU ; Rui-xia ZHU ; Qin YAN ; Wen FENG ; Rong-xia SUN ; Yan-fang ZHANG ; Xiao-fu WU ; Zheng-chuan FU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(1):72-73
Aged
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Biopsy, Needle
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methods
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Coal Mining
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Humans
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Lung
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pathology
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Lung Neoplasms
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complications
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diagnosis
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Middle Aged
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Pneumoconiosis
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complications
8.Expression profiling of MicroRNAs in hippocampus of rats following traumatic brain injury.
Ting-Yi, SUN ; Xiao-Rui, CHEN ; Zi-Long, LIU ; Li-Li, ZHAO ; Yong-Xiang, JIANG ; Guo-Qiang, QU ; Rong-Shuai, WANG ; Si-Zhe, HUANG ; Liang, LIU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(4):548-53
The changes of microRNA expression in rat hippocampus after traumatic brain injury (TBI) were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI: 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regulated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice.
9.Background chloride currents in fetal human nasopharyngeal epithelial cells.
Xue-Rong SUN ; Li-Wei WANG ; Jian-Wen MAO ; Lin-Yan ZHU ; Si-Huai NIE ; Ping ZHONG ; Li-Xin CHEN
Acta Physiologica Sinica 2005;57(3):349-354
To characterize the background current in fetal human nasopharyngeal epithelial cells and clarify its relationship with volume activated Cl(-) currents (I(Cl,vol)), whole-cell patch clamp and cell imaging techniques were employed. Under isotonic conditions, a background current [(5.9+/-2.1) pA/pF at +80 mV, n=21] was detected. The current presented a weak outward rectification and a negligible time-dependent inactivation. The current-voltage relationship showed that the reversal potential of the background current [(-0.73+/-1.7) mV, n=21] was close to the calculated equilibrium potential for Cl(-)(-0.9 mV). Application of extracellular hypertonic stimulation (440 mOsmol/L) suppressed the current by (59.6+/-7.1)% and the inhibition was reversible after returned to isotonic conditions. Bathing the cells in hypotonic solution (160 mOsmol/L) induced a volume-sensitive Cl(-) current. The Cl(-) channel blockers, tamoxifen (20 micromol/L) and 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) (100 micromol/L), inhibited the background current by (74.0+/-5.2)% (P<0.01, n=5) and (60.9+/-8.9)% (P<0.01, n=6) at +80 mV and increased basal cell volume by (107.7+/-2.9)% (P<0.01, n=25) and (104.4+/-2.4)% (P<0.01, n=19), respectively. The data indicate that Cl(-) current is an important component of the background current in fetal human nasopharyngeal epithelial cells. The background Cl(-) current is involved in volume activated Cl(-) current and basal cell volume regulation.
Cells, Cultured
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Chloride Channels
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antagonists & inhibitors
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physiology
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Electrophysiology
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Epithelial Cells
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cytology
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metabolism
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physiology
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Fetus
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Humans
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Nasopharynx
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cytology
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Nitrobenzoates
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pharmacology
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Patch-Clamp Techniques
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Tamoxifen
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pharmacology
10.Preoperative induction chemotherapy for unresectable stage IIIA non-small-cell lung cancer.
Si-yu WANG ; Zhi-fan ZENG ; Wei OU ; Yong-bin LIN ; Tie-hua RONG
Chinese Journal of Oncology 2005;27(12):747-749
OBJECTIVETo evaluate the potential reconsideration of curative operative treatment for patients with unresectable stage IIIA (N2) non-small-cell lung cancer (NSCLC).
METHODSFrom Jan. 1999 to Dec. 2002, 76 patients with unresectable stage IIIA (N2) NSCLC were entered in this study. They had all been proved by chest CT, chest film and fiberobronchoscopy. Twenty-one (27.6%) patients were examined by mediastinoscopy. All the patients received two cycles of chemotherapy with NVB (25 mg/m(2), D1, D5) and carboplatin (300 mg/m(2), D1). All the patients were staged again three weeks after induction chemotherapy. Sixty-four patients who achieved partial response (PR) or complete response (CR) were allowed to undergo surgery. Twelve patients who did not responde to chemotherapy received radiotherapy instead. Of the 64 surgically treated patients, 56 (84.7%) had a complete resection and then received 2 cycles of chemotherapy using the same regime, 8 patients had an incomplete resection and then received radiotherapy for the residual tumor.
RESULTSThe median survival for these 76 patients with unresectable stage IIIA (N2) NSCLC treated by either surgery or radiation after induction chemotherapy was 18.6 months with 1-, 2-, 3-year survival rate of 64.2%, 39.4% and 25.6%, respectively. The median survival for the 56 patients with a complete resection was 28.2 months with 1-, 2-, 3-year survival rate of 70.4%, 52.5% and 38.6%, respectively.
CONCLUSIONPreoperative induction chemotherapy with NVB plus carboplatin should be seriously considered for the patients with unresectable stage IIIA (N2) NSCLC, It is suggested that, whenever possible, surgery should be taken as the first choice for the patients who show down-staged benefits that complete resection can be attempted.
Adult ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Carboplatin ; administration & dosage ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; surgery ; Chemotherapy, Adjuvant ; methods ; Combined Modality Therapy ; Drug Administration Schedule ; Female ; Humans ; Lung Neoplasms ; drug therapy ; surgery ; Male ; Mediastinoscopy ; Middle Aged ; Preoperative Care ; Vinblastine ; administration & dosage ; analogs & derivatives