1.Clinical Significance of Detection by Fluorescence in Situ Hybridization for Bladder Cancer
Chunlei MA ; Jin SI ; Cong DAI ; Dianyong ZHANG ; Jinyi LI
Chinese Journal of Clinical Oncology 2009;36(23):1329-1332
Objective: To analyze chromosome aberrations in bladder transitional cell carcinoma with exfoliated cells, and to evaluate the clinical value of fluorescence in situ hybridization (FISH) in bladder cancer. Methods: FISH was performed using centromeric probes of 3, 7, 17 and locus probe of p16 to examine chromosome aberrations of exfoliated cells of 56 bladder cancer patients and 20 healthy controls to analyze the correlation of chromosome aberration with the pathological features of bladder cancer. The urine cytology of the 56 bladder cancer patients was performed. Results: The rates of aneuploidy of chromosomes 3, 7, and 17 were 58.9%, 39.3%, 58.9% and 75.0% for aberration of p16 in exfoliated cells from the 56 bladder cancer patients. All of the aberrations had no correlation with tumor stage (P>0.05). The aberrations of chromosomes 3, 7, and 17 were significantly correlated with pathological grade (P<0.05). The sensitivity of the 4 chromosome probes for detecting bladder cancer was 80.4%. The detection rate of FISH was obviously higher than that of udne cytology. Conclusion: Chromosome aberration is correlated with the growth of bladder cancer. The detection of FISH has significance for early di-agnosis, prognosis evaluation, and recurrence monitoring of bladder cancer.
3.Reliability and Validity of Chinese Version ofWater Orientation Test of Alyn 1
Long JIN ; Fang CONG ; Yao CUI ; Dunwu XIAO ; Fengshan SI ; Bin YAO ; Wei JIA ; Youlin LI ; Shuzeng ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2015;21(5):539-543
Objective To translate the Water Orientation Test of Alyn 1 (WOTA1) into Chinese and investigate the test-retest reliability, inter-rater reliability and validity of this Chinese Version. Methods The Water Orientation Test of Alyn 1 was translated from English into Chinese. 25 children with cerebral palsy were tested with Chinese Version of WOTA1 by 2 raters. One of the raters accessed the same children again after 2 days. While the children were assessed with Brief Assessment of Motor Function (BAMF). The scores from both raters, twice of one rater, and of WOTA1 and of BAMF were analyzed with Pearson correlation coefficient. Results The correlation coefficient of test-retest of WOTA1 total score is 0.992 (95% CI: 0.982-0.996), and it was 0.887 to 1.000 of the scores of items. The correlation coefficient of inter-rater of total score is 0.992 (95% CI: 0.980-0.998), and it was 0.748 to 1.000 of the scores of items. The correlation coefficient between scores of WOTA1 and BAMF was 0.926 (P<0.01). Conclusion The Chinese Version of WOTA1 is good reliable and valid to assess the motor function in hydrotherapy pool.
4.Total body irradiation of the donor in a spontaneous tolerance rat liver transplantation model and the effects on CD4(+)CD25(+) regulatory T cells content.
Ye-wei ZHANG ; Dong-liang YAN ; Si-cong LU ; Xue-hao WANG ; Wan Y LAU
Chinese Journal of Surgery 2009;47(21):1658-1662
OBJECTIVETo study the effect of total body irradiation of the donor in a spontaneous tolerance rat liver transplantation model and the role of CD4(+)CD25(+) regulatory T cells on induction of immunotolerance in the recipient.
METHODSLiver transplantation was performed using male Lewis rats as donors and male DA rats as recipients. These rats were randomly allocated into the following groups:Control group, Homogeneity Liver Transplantation group, Idio-immunotolerance group and Acute Rejection group. After transplantation, survival time rate of each group were observed. Serum ALT, TB level, Foxp3(+)CD4(+)CD25(+) regulatory T cells, expression of GITR on T cell subgroup, histopathology of the hepatic graft on day 14, spleen CTL lytic activity on day 14 were measured.
RESULTSIn the Idio-immunotolerance group, the recipients suffered from transient rejection after surgery but acquired immunotolerance and survived long. In the Acute Rejection group, the donors were preconditioned with total body irradiation before liver transplantation. All recipients died between day 17 to 21. Serum ALT and TB increased significantly and the ratio of Foxp3(+)CD4(+)CD25(+) regulatory T cells decreased significantly compared with the Idio-immunotolerance group, the Homogeneity Liver Transplantation group and the Control group. The expression of GITR on CD3(+)CD4(+)T cells in the peripheral blood decreased, the expression of GITR on CD3(+)CD8(+) T cells and CTL lytic activity of the recipients increased by preconditioning of the donors with total body irradiation.
CONCLUSIONSPreconditioning of the donors with total body irradiation eliminated the passenger lymphocytes of the liver graft, decreased the expression of Foxp3(+)CD4(+)CD25(+) regulatory T cells in peripheral blood, and increased the expression of GITR on CD3(+)CD8(+) T cells, thus affected the course of tolerance and induced acute rejection after liver transplantation.
Animals ; Liver Transplantation ; immunology ; Male ; Rats ; Rats, Inbred Lew ; T-Lymphocytes, Regulatory ; immunology ; Tissue Donors ; Transplantation Conditioning ; Transplantation, Homologous ; immunology ; Whole-Body Irradiation
5.Influence of propofol and isoflurane on cytokines response to cancer surgery during perioperative period.
Cong YU ; Yu-lin LUO ; Shui-sheng XIAO ; Qing ZHANG ; Si-lu CHEN
West China Journal of Stomatology 2007;25(6):554-556
OBJECTIVETo compare the influence of propofol and isoflurane on pro-inflammatory and anti-inflammatory cytokine response to perioperative period of tongue cancer surgery.
METHODSTwenty-four adult patients undergone the operation of tongue cancer were assigned to two groups randomly, propofol group (Group P) and isoflurane group (Group I). In group P, anesthesia was induced with fentanyl 2-3 microg/kg, propofol 2 mg/kg, atracurium 0.6 mg/kg and maintained with propofol 5-8 mg x kg(-1) x h(-1) and inhalation of 50% nirous oxide (N2O:O2=50%:50%). In group I, anesthesia was induced with 3%-4% isoflurane, fentanyl 2-3 microg/kg, diazepam 0.06-0.1 mg/kg, atracurium 0.6 mg/kg and maintained with inhalation of 50% N2O and isoflurane (ended-tidal isoflurane was maintained at 0.6%), in two groups atracurium was given intermittently. Blood samples were taken from peripheral vein before anesthesia (TO), at the end of operation (T1), 24 h (T2) and 48 h (T3) after operation for determination of serum IL-6 and IL-10 concentrations. The mean arterial pressure (MAP) and body temperature in two groups were recorded.
RESULTSIL-6 and IL-10 levels increased significantly in two groups at T1, T2 and T3 compared with T0 (P < 0.01). The increasing trend of IL-6 and IL-10 levels were similar in both groups, whereas the level of IL-6 at T1 in propofol group was lower than that of isoflurane group significantly (P < 0.01), however the level of IL-10 was much higher in propofol group than that of isoflurane group at T1 and T2 (P < 0.05).
CONCLUSIONThe influence of total intravenous anesthesia of propofol on post-operation inflammatory response is much gentler than isoflurane.
Adult ; Anesthesia, Intravenous ; Cytokines ; Female ; Humans ; Interleukin-10 ; Interleukin-6 ; Isoflurane ; Male ; Middle Aged ; Neoplasms ; Perioperative Period ; Postoperative Period ; Propofol
6.Relationship between beta amyloid protein 1-40 and post-operative delirium after oral and maxillofacial surgery in elderly patients.
Cong YU ; Qing ZHANG ; Si-lu CHEN ; Yu-lin LUO ; Shui-sheng XIAO
West China Journal of Stomatology 2010;28(5):498-501
OBJECTIVETo determine the incidence of post-operative delirium after oral and maxillofacial surgery under general anesthesia in elderly patients, and to examine its association with plasma concentrations of beta amyloid protein 1-40 (Abeta1-40).
METHODSFifty patients underwent elective oral and maxillofacial surgery were divided into two groups: Group C (n=20) aged from 20 to 60 years old, and Group T (n=30) aged from 62 to 78 years old. The two group received the same general anesthesia. Delirium rating scale-revised-98 (DRS-R-98) was used as an instrument to diagnose and access the postoperative delirium of the two groups. The scores of DRS-R-98 were recorded before operation (T0) and at 24 h (T1), 48 h (T2), 72 h(T3) and 96 h(T4) after the operation. Serial measurements of serum concentrations of Abeta1-40 were also performed at the same time.
RESULTSThe incidence of post-operative delirium after oral and maxillofacial surgery in Group T was 20.0%. The concentrations of plasma Abeta1-40 in group T were much higher than group C at TO, T1, T2 and T3 significantly (P < 0.01). The concentrations of plasma Abeta1-40 at T1 and T2 were higher than those at TO in the same group (P < 0.05). The scores of DRS-R-98 in Group T at T3 and T4 were much higher than those at T1 and Group C significantly (P < 0.01).
CONCLUSIONThe constant increase of plasma Abeta1-40 may be one of the important factors related to post-operative delirium in elderly patients underwent oral and maxillofacial surgery.
Aged ; Amyloid beta-Peptides ; Delirium ; Female ; Humans ; Male ; Peptide Fragments ; Surgery, Oral
7.Causes of ineffectiveness of microvascular decompression for hemifacial spasm.
Yue YUAN ; Li ZHANG ; Si-xun ZHANG ; Yan-bing YU ; Guo-qiang CHEN ; Kui-ming ZHAO ; Jing GUO ; Huan-cong ZUO
Chinese Journal of Surgery 2003;41(5):362-364
OBJECTIVETo study causes of ineffectiveness of microvascular decompression (MVD) in treatment of hemifacial spasm (HFS).
METHODSReoperative MVD was performed in 23 HFS patients with previous ineffective MVD. In the patients, the main causes of ineffectiveness included misjudgment of compressing vessels (7 patients), improper insertion of decompressing grafts (9), improper selection of grafts (5) and small grafts (2).
RESULTSSymptoms of HFS disappeared immediately after the second MVD in 21 patients and delayed in 2 patients (after 2 weeks, 6 weeks). No recurrence of HFS was noted during the follow-up period of 1.0 - 6.0 years (mean 3.4 years).
CONCLUSIONSMVD is an effective microsurgical method for treating HFS. Accurate judgement of compressing vessels and proper decompression are the key to surgical effectiveness.
Adult ; Decompression, Surgical ; methods ; Female ; Follow-Up Studies ; Hemifacial Spasm ; surgery ; Humans ; Male ; Medical Futility ; Microsurgery ; methods ; Middle Aged ; Reoperation ; Treatment Outcome ; Vascular Surgical Procedures ; methods ; Young Adult
8.Retrospective review of 190 patients treated for parotid tumors: a single institute experience.
Jing-jia LI ; Ge-hua ZHANG ; Xian LIU ; Jin YE ; Qin-tai YANG ; Jian-cong HUANG ; Si WU ; Gen-di YIN
Chinese Medical Journal 2013;126(5):988-990
Adult
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Female
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Humans
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Male
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Middle Aged
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Parotid Neoplasms
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diagnosis
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pathology
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surgery
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Retrospective Studies
9.Rebound depolarization of substantia gelatinosa neurons and its modulatory mechanisms in rat spinal dorsal horn.
Ling-Chao LI ; Da-Ying ZHANG ; Si-Cong PENG ; Jing WU ; Chang-Yu JIANG ; Tao LIU
Journal of Southern Medical University 2016;37(2):204-209
OBJECTIVETo investigate the rebound depolarization of substantia gelatinosa (SG) neurons in rat spinal dorsal horn and explore its modulatory mechanisms to provide better insights into rebound depolarization-related diseases.
METHODSParasagittal slices of the spinal cord were prepared from 3- to 5-week-old Sprague-Dawley rats. The electrophysiologic characteristics and responses to hyperpolarization stimulation were recorded using whole-cell patch-clamp technique. The effects of hyperpolarization-activated cyclic nucleotide gated cation (HCN) channel blockers and T-type calcium channel blockers on rebound depolarization of the neurons were studied.
RESULTSA total of 63 SG neurons were recorded. Among them, 23 neurons showed no rebound depolarization, 19 neurons showed rebound depolarization without spikes, and 21 neurons showed rebound depolarization with spikes. The action potential thresholds of the neurons without rebound depolarization were significantly higher than those of the neurons with rebound depolarization and spikes (-28.7∓1.6 mV vs -36.0∓2.0 mV, P<0.05). The two HCN channel blockers CsCl and ZD7288 significantly delayed the latency of rebound depolarization with spike from 45.9∓11.6 ms to 121.6∓51.3 ms (P<0.05) and from 36.2∓10.3 ms to 73.6∓13.6 ms (P<0.05), respectively. ZD7288 also significantly prolonged the latency of rebound depolarization without spike from 71.9∓35.1 ms to 267.0∓68.8 ms (P<0.05). The T-type calcium channel blockers NiCl2 and mibefradil strongly decreased the amplitude of rebound depolarization with spike from 19.9∓6.3 mV to 9.5∓4.5 mV (P<0.05) and from 26.1∓9.4 mV to 15.5∓5.0 mV (P<0.05), respectively. Mibefradil also significantly decreased the amplitude of rebound depolarization without spike from 14.3∓3.0 mV to 7.9∓2.0 mV (P<0.05).
CONCLUSIONNearly two-thirds of the SG neurons have rebound depolarizations modulated by HCN channel and T-type calcium channel.
Action Potentials ; Animals ; Calcium Channel Blockers ; pharmacology ; Calcium Channels, T-Type ; Cell Polarity ; Cesium ; pharmacology ; Chlorides ; pharmacology ; Cyclic Nucleotide-Gated Cation Channels ; antagonists & inhibitors ; Neurons ; cytology ; Patch-Clamp Techniques ; Pyrimidines ; pharmacology ; Rats ; Rats, Sprague-Dawley ; Spinal Cord Dorsal Horn ; cytology ; Substantia Gelatinosa ; cytology
10.Emodin-induced increase in expression of β1 subunit of BKCa channel mediates relaxation of cerebral basilar artery in spontaneously hypertensive rats.
Chuan-Lin ZHANG ; Li-Na CONG ; Rui WANG ; Yang WANG ; Ke-Tao MA ; Lei ZHAO ; Jun-Qiang SI ; Li LI
Acta Physiologica Sinica 2014;66(3):289-294
The purposes of this study were to investigate the effect of emodin on expression of BKCa channel β1 subunit in basilar artery smooth muscle cells (BASMCs) and electrophysiological characteristics of vascular smooth muscle cells in spontaneously hypertensive rats (SHR). Tail artery pressure measurement instrument was used to measure the change of SHR systolic blood pressure before and after emodin intervention. Single vascular smooth muscle cell was electrically recorded by whole-cell patch-clamp technique. Immunohistochemistry and Western blotting were used to study the distribution and expression of the BKCa channel β1 subunit. The results showed that emodin decreased blood pressure of SHR from (223 ± 16) mmHg to (127 ± 12) mmHg (P < 0.01). There was no difference of blood pressure between emodin-treated SHR and Wistar rats. Emodin significantly increased outward currents of smooth muscle cells in SHR (P < 0.05), and this effect could be reversed by specific inhibitor of BKCa channel, IbTX. Emodin also up-regulated BKCa channel β1 subunit expression in BASMCs. These results suggest that emodin relaxes cerebral basilar artery by enhancing BKCa current via increasing β1 subunit expression in BASMCs.
Animals
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Basilar Artery
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cytology
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Blood Pressure
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Emodin
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pharmacology
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Large-Conductance Calcium-Activated Potassium Channel alpha Subunits
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metabolism
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Myocytes, Smooth Muscle
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metabolism
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Patch-Clamp Techniques
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Rats
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Rats, Inbred SHR
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Rats, Wistar
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Vasodilation
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Vasodilator Agents
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pharmacology