1.In vivo comparison analysis of scandoside methyl ester metabolites in four kinds of liver microsomes using ultra-performance liquid chromatography combined with electrospray ionization tandem orbitrap mass spectrometry.
Yan LI ; Hongjie WANG ; Jing HE ; Nan SI ; Jian YANG ; Haiyu ZHAO ; Baolin BIAN
Acta Pharmaceutica Sinica 2014;49(9):1315-9
In order to clarify the metabolism pathways of scandoside methyl ester, the analysis of metabolites profiling in four kinds of liver microsomes was performed by using an ultra-performance liquid chromatography/ electrospray-tandem mass spectrometry (UPLC-ESI-MS). The data obtained from the 0 h-incubation and the 2 h-incubation were compared and analyzed. After incubation, 5 metabolites of scandoside methyl ester were found in rat, Beagles, rhesus monkey and human liver microsome. The results showed that scandoside methyl ester's major metabolic pathway in the liver microsomes is hydrolysis, oxidation and reduction reactions, and there are certain kinds differences between species. The study provides a research base for further research about iridoid compounds in vivo metabolic pathways.
2.Chemical constituents of bufadienolides in cinobufacino injection.
Lingyu HAN ; Nan SI ; Junqiu LIU ; Haiyu ZHAO ; Jian YANG ; Baolin BIAN ; Hongjie WANG
Acta Pharmaceutica Sinica 2014;49(11):1574-7
Cinobufacino injection is purified from water extraction of the skin of Bufo bufo gargarizans, which has been widely used for various cancers in clinic with significant anti-tumor effects. Bufadienolides were regarded as the main active constituents of cinobufacino injection in previous reports. In present study, 6 bufadienolides were isolated and purified from Cinobufacino injection. Their structures were identified as 3-epi-ψ-bufarenogin (1), ψ-bufarenogin (2), 3-epi-arenobufagin (3), arenobufagin (4), 3-epi-gamabufotalin (5), and 3-oxo-arenobufagin (6), separately. Among them, 1 and 3 were new compounds, 5 and 6 were new natural products. Compounds 1, 2 and compounds 3, 4 were two pairs configuration isomers at C-3, separately.
3.Comparison of the chemical composition between fresh and dried venenum bufonis by UPLC-Orbitrap MS.
Jing HE ; Yan LI ; Nan SI ; Haiyu ZHAO ; Baolin BIAN ; Hongjie WANG
Acta Pharmaceutica Sinica 2014;49(10):1446-50
To identify the active components in Bufo melanostictus Schneider and clarify the difference between fresh and dried Venenum Bufonis, a UPLC-Orbitrap MS method has been established. The separation was performed with gradient elution of acetonitrile and water (with 0.1% formic acid) as mobile phase. By comparing their retention time and high resolution mass data of Venenum Bufonis extracts, 39 effective components were primarily identified by MS/MS analysis in positive ion mode. Twenty-six of them were bufadienolides. There were significant differences in the main composition between fresh and dried Venenum Bufonis. There are fewer bufadienolides in fresh toad venom.
4.Identification of bufadienolides profiling in cinobufacino by HPLC-DAD-FT-ICR-MS method.
Jun-Qiu LIU ; Nan SI ; Jian YANG ; Hai-Yu ZHAO ; Bao-Lin BIAN ; Hong-Jie WANG
Acta Pharmaceutica Sinica 2014;49(2):244-248
Cinobufacino injection is a significant anti-tumor medicine for the treatment of various tumors in clinic, which was made from water extraction of the skin of Bufo bufo gargarizans. In present paper, HPLC-DAD-FT-ICR-MS method was used to identify the major bufadienolides in cinobufacino for the first time. Solid-phase extraction with dichloromethane and silica was used to enrich the total bufadienolides in cinobufacino. Based on the UV and high resolution MS/MS data, 33 bufadienolides were analyzed and characterized. Among them, eight compounds were identified by comparing with standard references unambiguously. This study elucidated the major bufadienolides in cinobufacino, which provided material foundation of cinobufacino and will be benefit for the further pharmacological research.
Amphibian Venoms
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chemistry
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Animals
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Bufanolides
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analysis
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chemistry
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Bufo bufo
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Chromatography, High Pressure Liquid
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Molecular Structure
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Spectrometry, Mass, Electrospray Ionization
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Tandem Mass Spectrometry
5.LC-FT-ICR-MS analysis of the prototypes and metabolites in rat plasma after administration of huang-lian-jie-du decoction.
Ran ZUO ; Hong-Jie WANG ; Nan SI ; Hai-Yu ZHAO ; Jian YANG ; Bao-Lin BIAN
Acta Pharmaceutica Sinica 2014;49(2):237-243
To obtain chemical constituent information of rat plasma after oral administration of Huang-Lian-Jie-Du Decoction (HLJDD), a LC-FT-ICR-MS method has been established, and both positive and negative ions scan modes were include in the analysis. By comparing their retention time, high resolution mass data of HLJDD extracts, blank plasma and dosed plasma, 38 constituents, including 22 prototype compounds and 16 metabolites, were detected in rat plasma after oral administration of HLJDD. In the 22 prototype compounds, 16 constituents were determined unambiguously by comparing with references. In the analysis of metabolites, phase II reactions like glucuronidation and sulfation were the major biotransformation pathways of HLJDD. M11 was observed as the only phase I metabolite in present experiment. The results will be beneficial for the further pharmacokinetics and pharmacological evaluations of HLJDD.
Administration, Oral
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Alkaloids
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blood
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Animals
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Biotransformation
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Chromatography, High Pressure Liquid
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Drug Combinations
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Drugs, Chinese Herbal
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administration & dosage
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isolation & purification
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metabolism
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Flavonoids
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blood
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Iridoids
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blood
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Male
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Plants, Medicinal
;
chemistry
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Rats
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Rats, Sprague-Dawley
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Spectrometry, Mass, Electrospray Ionization
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Tandem Mass Spectrometry
6.Recent researching progress of Lepidium meyenii (Maca).
Yan-yan ZHOU ; Hai-yu ZHAO ; Nan SI ; Hong-jie WANG ; Bao-lin GIAN
China Journal of Chinese Materia Medica 2015;40(23):4521-4530
Maca as one of the star products in the international health care market in recent years, had a wide range of application value and promoted to all over the world. However, the basic research of Maca was not deep, lack of systematic and clear efficacy studies. Market products hype its aphrodisiac effect, which greatly impact more systematic in-depth research and exploration. Therefore, this paper briefly summarizes advance research in recent years including the status quo of the resources, growth cultivation, phytochemical, pharmacological effect and other aspects, which can provide reference for rational development and utilization of Maca.
Animals
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Biomedical Research
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Humans
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Lepidium
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chemistry
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classification
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growth & development
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Plant Extracts
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chemistry
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metabolism
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pharmacology
7.The selection of window width and level for measuring the airway dimensions with spiral CT scan: an experimental study in Japanese white big-ear rabbits
Xinwei HAN ; Huibin LU ; Gang WU ; Ji MA ; Nan WANG ; Jiangtao SI
Journal of Interventional Radiology 2009;18(9):695-698
Objective To investigate the optimal window width and window level for measuring the airway dimensions with spiral CT scan in Japanese white big-ear rabbits so as to lay the foundation for airway stenting in animal experiments. Methods Multi-slice spiral CT scanning of cervico-thoracic region was performed in 30 healthy adult Japanese white big-ear rabbits, the anteroposterior and transversal diameter of the thoracic trachea, the anteroposterior diameter of the right and left bronchus were measured with lung window, mediastinum window and special fat window separately. The revealing rate of the tracheal wall and the measuring results in different windows and levels were recorded and compared with the anatomical data. The differences of the relevant data were statistically analyzed. Results With lung window, the tracheal wall was well demonstrated, but the relevant data were smaller than that with mediastinum window. With mediastinum window, the data were bigger and the tracheal wall border appeared blurred. The results obtained with fat window were close to the actual anatomical data. Conclusion For accurately measuring the anteroposterior and transversal diameter of the thoracic trachea in Japanese white big-ear rabbits with multi-slice spiral CT scan, fat window should be adopted, which is helpful for the preparation of tracheal and bronchial stents.
8.LC-FT-ICR-MS analysis of the prototypes and metabolites in rat plasma after administration of huang-lian-jie-du decoction.
Ran ZUO ; Hongjie WANG ; Nan SI ; Haiyu ZHAO ; Jian YANG ; Baolin BIAN
Acta Pharmaceutica Sinica 2014;49(2):237-43
To obtain chemical constituent information of rat plasma after oral administration of Huang-Lian-Jie-Du Decoction (HLJDD), a LC-FT-ICR-MS method has been established, and both positive and negative ions scan modes were include in the analysis. By comparing their retention time, high resolution mass data of HLJDD extracts, blank plasma and dosed plasma, 38 constituents, including 22 prototype compounds and 16 metabolites, were detected in rat plasma after oral administration of HLJDD. In the 22 prototype compounds, 16 constituents were determined unambiguously by comparing with references. In the analysis of metabolites, phase II reactions like glucuronidation and sulfation were the major biotransformation pathways of HLJDD. M11 was observed as the only phase I metabolite in present experiment. The results will be beneficial for the further pharmacokinetics and pharmacological evaluations of HLJDD.
9.Identification of bufadienolides profiling in cinobufacino by HPLC-DAD-FT-ICR-MS method.
Junqiu LIU ; Nan SI ; Jian YANG ; Haiyu ZHAO ; Baolin BIAN ; Hongjie WANG
Acta Pharmaceutica Sinica 2014;49(2):244-8
Cinobufacino injection is a significant anti-tumor medicine for the treatment of various tumors in clinic, which was made from water extraction of the skin of Bufo bufo gargarizans. In present paper, HPLC-DAD-FT-ICR-MS method was used to identify the major bufadienolides in cinobufacino for the first time. Solid-phase extraction with dichloromethane and silica was used to enrich the total bufadienolides in cinobufacino. Based on the UV and high resolution MS/MS data, 33 bufadienolides were analyzed and characterized. Among them, eight compounds were identified by comparing with standard references unambiguously. This study elucidated the major bufadienolides in cinobufacino, which provided material foundation of cinobufacino and will be benefit for the further pharmacological research.
10.Advances in Event-related Potential and Its Forensic Application Value.
Nan-si GUAN ; Ji-hui LIU ; Xin-yuan ZHANG ; Wan WANG ; Ja-ning TAN ; Bo PENG
Journal of Forensic Medicine 2015;31(2):135-139
The event-related potential (ERP) is considered as one of the most effective methods to study and analyze objectively human mental activity based on nerve electrophysiology. At present, ERP is not only used in the study of lie detection, but also in the clinical medicine for the cognitive assessment on patients with cerebrovascular disease, dementia or traumatic brain injury and auxiliary diagnosis of mental illness. With the further development of ERP detection technology, it would have a wider application prospect in the field of forensic medicine.
Evoked Potentials/physiology*
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Forensic Medicine/trends*
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Humans