OBJECTIVETo research the distribution characteristics of Chinese medicine (CM) syndrome and syndrome elements of chronic fatigue syndrome (CFS) by analyzing literature in recent 20 years.

METHODSRelevant literature on treating CFS by syndrome differentiation of CM at home were retrieved by computer and manual ways. Database were established by using EpiData 3.1 to conduct frequency analysis of syndrome and syndrome elements.

RESULTSThe most common clinical syndromes were Xin-Pi deficiency syndrome, Gan stagnation Pi deficiency syndrome, Gan-Shen yin deficiency syndrome, Gan qi stagnation syndrome, and Pi-Wei qi deficiency syndrome. Disease locations were sequenced as Pi, Gan, Shen, and Xin. The clinical pathogenesis of CFS was characterized by deficiency of vital energy, complicated with intermingled excess and deficiency. Asthenia of healthy energy was mainly manifested as qi deficiency, blood deficiency, and yin deficiency, while excess of sthenia was mainly manifested as qi stagnation, phlegm dampness, and static blood.

CONCLUSIONSResearch of CM syndrome starting from syndrome elements can better unify and standardize clinical syndrome differentiation. Results of literature analysis can provide reference for further studies.

Fatigue Syndrome, Chronic ; Humans ; Medicine, Chinese Traditional ; Yang Deficiency ; Yin Deficiency

Purpose To investigate the expression of miR200a in different lung cancer cell lines and its effect on proliferation,migration,and apoptosis in A549 cells.Methods The expressions of miR-200a in different lung cancer cell lines were detected by RT-PCR.miR-200a mimics was transfected into A549 cells by Lipofectamine RNAiMax.The change of proliferation ablility of A549 cells was detected by CCK-8 method and plate clone formation assay.Cell migration was examined by Transwell chamber assay.The flow cytometry was used to examine the changes of apoptosis.The possible target genes of miR-200a were forecasted by bioinformatics tools.Results The results of RT-PCR showed that the expression of miR-200a was significantly down-regulated in A549,H23 and H460 cell lines than 16HBE cell line.CCK-8 assay showed that the OD values of the mimics group at 4,and 5 days were significantly lower than those in the negative control (NC) group (P ＜ 0.05).Plate clone formation assay showed rate of colony formation in the mimics group was significantly lower than that in the NC group [(33.13±0.74)％ vs (45.57 ±1.27)％,P＜0.05].Transwell migration assay showed that the cell number of mimics group that passed the Transwell membrane was significantly lower than that of the NC group [(71.60 ± 17.90) vs (140.20 ± 17.88),P ＜0.05].Flow cytometry showed that the apoptosis rate of the mimics group was significantly higher than that of the NC group [(17.80± 1.90)％ vs (11.33 ± 1.96)％,P ＜ 0.05].Tiam1 may be one of the target gene of miR-200a.Conclusion miR-200a can inhibit the proliferation and migration,and promote apoptosis of lung cancer A549 cells,suggesting a potential new therapeutic agent for lung cancer cell.MiR-200a may play a function of regulation of tumor development through target gene Tiam1.

OBJECTIVES: The purpose of this study was to compare the oral antimicrobial effects of seven different mouthwashes available in Korea. METHODS: To examine the antimicrobial effects of the seven mouthwashes, their minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined using broth microdilution methods. Streptococcus mutans ATCC 25175, Lactobacillus acidophilus ATCC 4355, Candida albicans KCTC 7270, and Porphyromonas gingivalis ATCC 33277 were used in this experiment. S. mutans and P. gingivalis were examined using a scanning electron microscope (SEM), after treatment with the mouthwashes containing cetylpyridinium chloride (CPC). RESULTS: Mouthwashes containing CPC had lower MIC and MBC values against the four microorganisms. Their bactericidal effects were concentration-dependent. S. mutans and C. albicans were highly sensitive to the concentration of CPC in the mouthwashes. According to the SEM observation, the treatment of bacteria with mouthwashes containing CPC, changed the cell surface texture of S. mutans and P. gingivalis. CONCLUSIONS: Mouthwashes containing CPC showed relatively lower MIC and MBC values under the same conditions against the four microorganisms used in this study.
Bacteria ; Candida albicans ; Cetylpyridinium ; In Vitro Techniques* ; Korea* ; Lactobacillus acidophilus ; Mouthwashes* ; Porphyromonas gingivalis ; Streptococcus mutans

OBJECTIVES: The purpose of this study was to provide photodynamic bactericidal effect against Enterococcus faecalis by erythrosine concentrations and LED irradiation times. METHODS: Erythrosine was used as a photosensitizer and green LED (3 Watt, 520-530 nm) was used as light source. E. faecalis ATCC 1943 and E. faecalis ATCC 29212 were used in this study. Approximately 10(5) CFU of bacteria were added in wells of a 96-well microtitration plate. For examining the effects of concentrations of erythrosine, 0, 0.625, 1.25, 2.5, 5, and 10 microM of erythrosine were added in wells containing bacteria. The irradiation time with LED was 30 sec. In another set of experiment, the effect of irradiation time for killing of bacteria was investigated by increasing irradiation time from 0 to 30 s with 10 microM of erythrosine final concentration. After irradiation, each sample was serially diluted with PBS and 50 microl of diluents was spread on duplicate blood agar plates. The plates were incubated for 72 h at 37degrees C under aerobic conditions and the number of CFU was determined. The experiments were repeated four times. The results were analyzed using one-way ANOVA, and Tukey's multiple comparison at a significance level of 0.05. RESULTS: When the erythrosine concentrations were more than 2.5 microM, E. faecalis ATCC 29212 was significantly decreased (P<0.05). The more erythrosine concentrations increased, the more E. faecalis ATCC 1943 decreased statistically significantly (P<0.05). In another set of experiment, when LED irradiation time was more than 20 s, E. faecalis ATCC 1943 decreased significantly (P<0.05), and if the irradiation times was more than 5 s, E. faecalis ATCC 29212 decreased significantly (P<0.05). CONCLUSIONS: PDT using erythrosine and green LED was found to be an effective method in killing E. faecalis.
Agar ; Bacteria ; Enterococcus faecalis* ; Erythrosine* ; Homicide ; Photochemotherapy

OBJECTIVEThree-dimensional (3-D) facial images are very useful for orthodontic diagnosis. Three-dimensional facial imaging system (DSC-2) based on phase measuring profilometry (PMP) was used to obtain images and tested for accuracy.

METHODSGeometric strips (20 mm in width) was used to test the accuracy of the obtained images. Three-dimensional images of plaster head model with marker of 5 mm x 5 mm were taken by DSC-2 three-dimensional facial imaging system. The difference of measurements was compared between both methods. Measurements were repeated twice by three different doctors.

RESULTSThe average value of width in 3-D mode was 19.5 mm. No significant difference was found, compared with actual width (20 mm). There was no significant difference between computer measurement and manual measurement of the plaster head model (P > 0.05).

CONCLUSIONSDSC-2 three-dimensional facial imaging system was accurate and reliable to obtain 3-D facial images.

Face ; anatomy & histology ; Humans ; Imaging, Three-Dimensional ; Photogrammetry ; methods

Objective:To understand and explore the detection rate of the antibodies of 9 types of a typical respiratory pathogens IgM in children with acute respiratory tract infection and the relationship with air pollutants.Methods:The data of air quality monitoring in Tianjin from June 2015 to May 2017 were recorded,and the serum of hospitalized children with ARI were collected during the same period.Detection the antibodies of 9 types of atypical respiratory pathogens IgM by method of indirect immunofluorescence.To understand the detection rate of the antibodies of 9 types of atypical respiratory pathogens IgM and explore the relationship with air pollutants.Results:Among the 33 078 samples,8 364 samples were positive and total positive rate was 25.29%.ADV accounted for 152(0.46%) in all these samples,COX accounted for 9(0.03%),CPN accounted for 14(0.04%),FLuA accounted for 3(0.01%), FLuB accounted for 930(2.8%),LP accounted for 96(0.29%),MP accounted for 6 719(20.3%),PIV accounted for 326(0.99%), RSV accounted for 115(0.35%).The MP and FLuB were major pathogens.During the survey,the average PM2.5,PM10 and NO2were all at a high level,with PM2.5 being the highest and PM10 being the second highest.The AQI and the concentration of PM2.5,PM10, NO2and SO2had correlation with hospitalized cases,the concentration of PM10,NO2,SO2had correlation with and the total positive samples,and the correlation between the concentration of SO2and MP was the closest.Conclusion: The positive rate of MP was the hightest in hospitalized children with ARI of Tianjin children's hospital from June 2015 to May 2017,followed by FluB.Tianjin has severe air pollution,AQI and air pollutants has correlation with the number of hospitalized patients and the total positive samples, correlation between the concentration of SO2and MP was the closest.

0.05).Conclusion GH improves FAH of children with GHD.Height at the initiation of puberty is the most significant determining factor for the long-term efficacy.Hence,it is important that the diagnosis should be made and treatment be initiated as early as possible to afford children with GHD the opportunity to make up much of their height deficit before puberty.Adequate dosage of GH should be used for the children taking initial treatment at puberty to attain satisfactory FAH.

The aim of this study was to evaluate the therapeutic effects and adverse reactions of fludarabine-based regimen for patients with chronic lymphocytic leukemia(CLL).18 patients with CLL were treated with F regimen [fludarabine 30 mg/(m(2)·d) intravenously for 3 d, repeatedly every 28 days]. 22 patients were treated with FC regimen [fludarabine 25 mg/(m(2)·d) plus cyclophosphamide 250 mg/(m(2)·d) intravenously for 3 d, repeatedly every 28 days]. The results showed that the rate of complete remission (CR), partial remission (PR) and overall remission (OR) reached 16.7%, 61.1% and 77.8% in the F regimen groups and 59.1%, 40.9% and 100% in the FC regimen groups (P < 0.05, P > 0.05 and P > 0.05), respectively. FC regimen resulted in significantly higher CR rate than that in single-agent fludarabine regimen. The main adverse reactions were myelosuppression and immunosuppression. No significant differences were found between the two regimens. FC regimen did not increase the rate of severe infections. It is concluded that FC regimen can give higher CR rate as compared with F regimen, fludarabine-based regimens is effective and safe first-line regimen for patients with CLL.
Adult ; Aged ; Aged, 80 and over ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Cyclophosphamide ; administration & dosage ; Female ; Humans ; Leukemia, Lymphocytic, Chronic, B-Cell ; drug therapy ; Male ; Middle Aged ; Treatment Outcome ; Vidarabine ; administration & dosage ; analogs & derivatives

OBJECTIVETo investigate the protective action of Epimedium against chemotherapy-induced damage to rat epididymides.

METHODSFifty 60-day-old male rats were divided into a control, a model and a treatment group. Procarbazine was injected into the abdominal cavity of the model rats at the dose of 30 mg/(kg x d). In addition to procarbazine, Epimedium was given intragastrically to the treatment group. The changes in the ultrastructure of the epididymis were observed after 10 and 20 days.

RESULTSElectron microscopy showed that the chemotherapy-induced damages to the epididymal epithelia mainly included cell swelling, local cavitation of mitochondria, tumor-like change in nucleoli, agglutination of marginal translocation of heterochromatin and cell apoptosis. The damage to the epithelial ultrastructure was slight in the treatment group as compared with the model rats. Chemotherapy significantly affected sperm concentration, sperm viability and sialic acid (SA), which were (15.59 +/- 4.01) x 10(6)/ml, (76.71 +/- 10.11)% and (19.38 +/- 9.34) g/mg prot in the model group in comparison with (10.63 +/- 3.82) x 10(6)/ml (P < 0.01), (60.03 +/- 7.54)% (P < 0.01) and (13.62 +/- 7.81) g/g prot (P < 0.05) in the control. Epimedium significantly increased sperm viability in the treatment group (60.03 +/- 7.54)% as compared with the model rats (69.90 +/- 12.58)% (P < 0.05).

CONCLUSIONEpimedium can lessen chemotherapy-induced damage to the epididymis and protect the reproductive function of rats.

Animals ; Antineoplastic Agents ; toxicity ; Drugs, Chinese Herbal ; pharmacology ; Epididymis ; drug effects ; physiopathology ; ultrastructure ; Epimedium ; chemistry ; Infertility, Male ; chemically induced ; physiopathology ; prevention & control ; Male ; Microscopy, Electron, Transmission ; Phytotherapy ; Random Allocation ; Rats ; Rats, Sprague-Dawley

To identify the active constituents in vitro and blood-absorbed ingredients in vivo from Yin Chen Hao decoction provides scientific evidence for probing its prevention and treatment mechanism on acute liver injury. An ultrahigh performance liquid chromatography quadrupole-time of flight-mass spectrometry (UPLC-QTOF/MS) method was applied for analysis of Yin Chen Hao decoction and the serum samples of mice with con-A induced acute liver injury after preventive oral administration for 14 days (the use of all laboratory animals in this study was approved by the Ethics Committee of the Naval Medical University, 19YF1459400). A total of 90 chemical constituents were identified from Yin Chen Hao decoction, mainly were flavonoids, terpenoids, tannins, quinones. 5 prototype compounds were identified in the serum, including chrysophanol, deoxyrhapontin-8-O-gallate, mussaenosidic acid, herniarin, emodin. The established UPLC-QTOF/MS method could efficiently and sensitively identify the constituents in vitro and blood-absorbed ingredients of Yin Chen Hao decoction, primarily clarify the material basis of its hepatoprotective effect, and provided a scientific basis for the quality marker selection and the pharmacodynamic material basis research on the decoction.