1.Genotype I Japanese encephalitis virus is the main genotype in mosquito in Fujian province.
Xiao-Xi HE ; Huan-Yu WANG ; Shi-Hong FU ; Ying HE ; Fa-Zhu YANG ; Wei-Xin CHEN ; Bao-Hai XU ; Su-Na LU ; Han-Guo XIE ; Si Su-Rong HA ; Yan-Sheng YAN ; Guo-Dong LIANG
Chinese Journal of Experimental and Clinical Virology 2012;26(2):81-83
OBJECTIVETo grasp the infection rate and genotypes of Japanese encephalitis virus (JEV) in mosquito in Fujian province.
METHODSMosquito specimens in Sanming city, Jianyang city and Fuzhou city in Fujian province were collected in 2010. RT-PCR was used to detect the JEV sequence from the mosquitoes by specific primers. The sequence splicing and the differentiation analysis for nucleotides, deduced amino acid sequence and phylogenetic tree were performed by the software of ATGC, Clustal X (1.83), MegAlign, GeneDoc 3.2 and Mega (4.0).
RESULTSTotally 6987 mosquitoes were collected and main species was Culex tritaeniorhynchus and Anopheles sinensis. The infection rate of JEV in mosquitoes in Sanming, Jianyang and Fuzhou were 1.25%, 1.76% and 0.65%, respectively. One full genome in the positive specimens was sequenced. And further study showed that the positive JEV sequences belonged to genotype I.
CONCLUSIONGenotype I Japanese encephalitis virus is the main genotype in mosquitos in Fujian province.
Animals ; Culicidae ; virology ; Encephalitis Virus, Japanese ; classification ; genetics ; Genotype ; Phylogeny ; Time Factors
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