Objective: To investigate the inhibitory effects of Curcumin on A? generation in vitro and explore its potential mechanisms involing BACE1 and PPAR?.Methods: Plasmids APPswe and BACE1-mychis were transiently co-transfected into SHSY5Y cells by LipofectaminTM2000,then treated with Curcumin at 0(control Group),1.25,5.0,20.0 ?mol/L for 24 hr,or with Curcumin at 5 ?mol/L for 0,12,24 and 48 hrs for the time course assay.RT-PCR was performed to measure the endogenous levels of BACE1 mRNA and PPAR?.Western blot were used to detect the protein expression of BACE1 and PPAR?.The major cleavage product——A?40 and A?42 was detected by ?-amyloid 1-40 or 42 Colorimetric ELISA assay.Results: The mRNA level and protein level of BACE1 were decreased obviously in a dose-and time-dependent manner after treated with Curcumin in the cell line(P

Alzheimer's disease (AD) is a common progressive degenerative disease of the central nervous system. It's also a main cause for dementia in presenium and senectitude. In the past few years, the discoveries in vitro expansion of neural stem cells (NSCs) indicate a new way for recovery and replacement of damaged neurons as well as reconstruction of the neural circuit, and offer a useful future therapy for AD. There are two main promising approaches in NSCs replacement therapy. They are endogenetic approach, inducing proliferation and differentiation of endogenetic NSCs, improving self-repair of central nervous system, and exogenous approach, ransplantation of exogenous tissue and promoting proliferation of endogenous NSCs. However, it may not become truth before the mechanism of NSCs' proliferation, migration, differentiation and it's integration with the host tissues to be elucidated.

Breast Neoplasms ; complications ; virology ; Epstein-Barr Virus Infections ; complications ; physiopathology ; virology ; Female ; Herpesvirus 4, Human ; Humans ; Statistics as Topic

Adenocarcinoma ; pathology ; Apoptosis ; drug effects ; Cell Line, Tumor ; Cell Survival ; drug effects ; Dose-Response Relationship, Drug ; Humans ; Nucleosides ; administration & dosage ; pharmacology ; Proto-Oncogene Proteins c-akt ; antagonists & inhibitors ; Pyridazines ; administration & dosage ; pharmacology ; Stomach Neoplasms ; pathology

Adolescent ; Adult ; Child ; Chronic Disease ; Female ; Humans ; Male ; Middle Aged ; Mucin-5B ; biosynthesis ; Nasal Mucosa ; metabolism ; Sinusitis ; metabolism ; Young Adult

Objective To compare the postoperative effect of pars plana vitrectomy,phacoemulsification and intraoeular lens (IOL)implantation with silicon oil removed,phacoemulsification and IOL implantation.Design A retrospective case-control study.Par- ticipants 91 cases(104 eyes)of proliferative diabetic retinopathy with tractive retinal detachment or vitreous hemorrhage were per- formed cataract surgery with IOL implantation.Methods All patients were followed up average 6 months,in whom 52 eyes with pars plana vitrectomy,phacoemulsification and IOL implantation(combined group),and the other 52 eyes with silicon oil removed,pha- coemulsification and IOL implantation(sequential group).The differences of the postoperative visual acuity and inflammatory reactions of the anterior chamber between the two groups were analyzed.Main outcome measures Visual acuity and inflammatory reactions of the anterior chamber.Results There were 14/52 eyes with best corrected visual acuity more than 0.1 in combined group and 8/52 eyes in the sequential group after the surgeries(X~2=-6.87,P=0.07) .Corneal edema happened in 16/52 eyes in combined group and 6/52 eyes in sequential group 1 week after the surgeries(X~2=11.53,P=0.001).Keratic precipitate happened in 20/52 eyes in combined group and 9/52 eyes in sequential group after the surgeries(X~2=5.79,P=0.019) .Conclusions The incidence of postoperative anterior chamber in- flammation is higher in Phacoemulsification combined with pars plana vitrectomy group.

Micronucleus ( MN) assay as a routine examination for genotoxicity has been widely used.The testing specimens were taken from bone marrow and extended from blood and tissues.In addition to testing genotoxicity of drugs, it is also applied in disease diagnosis for genetic mutation, evaluation of curative effectiveness and disease prevention. Moreover, MN assay is also an important safety indicator for drugs and health foods registration.This review will discuss the staining method of MN test and its application in the field of diseases and virology.

P53 abnormality or mutation is commonly seen in patients with hepatocellular carcinoma (HCC), and therefore, restoration of P53 function has become a research hotspot in the treatment of HCC.This article reviews the association of P53 with Bcl-2 protein family, microRNA, TGFβ, HBV, HCV, and AKT and the role of P53 in regulating cell apoptosis, in order to provide clues for improving the therapeutic outcome of HCC.

This study aimed at exploring the modeling and identification method of autoimmune POF by the ZP3 polypeptide with the provision of scientific basis for the prevention and treatment of POF.B6AF1 female mice,the hybridization of A/J male mice and C57BL/6 female mice,was immunized by zona pellucida polypeptide fragments.Then,the mice estrous cycle was detected by vaginal exfoliated cells Pasteur's staining.After the 14-day modeling,the mice model was identified on the 7th and 14th day,respectively.Ovarian tissue morphology and anti-zona pellucida antibodies immunofluorescence change were detected by H&E staining and ZP3 immunofluorescence staining.Serum hormone levels of E2 and FSH in the mice were tested by ELISA,with the combination of estrous cycle,ovarian tissue morphology and anti-zona pellucida antibodies immunofluorescence change to judge the success of the POF mice model.It was found that ZP3 polypeptide immunized mice appeared the disorder of estrous cycle after 14 days,so did the marked inflammation observed by ovarian biopsy,a clear transparent zone by ZP3 immunofluorescence staining,and the decrease of serum E2 declined and the increase of serum FSH by ELISA.However,no significant change was found on the 7th day in the modeling.In conclusion,ZP3 polypeptide induced autoimmune POF mice model was established successfully,featuring high incidence and simple method and laying a foundation for the further study of autoimmune POF.