Child, Preschool ; Female ; Fructose Intolerance ; pathology ; Humans

Objective To explore tumor inhibitory effect of the lentiviral vectors mediated small interfering RNA(siRNA)targe‐ting survivin gene on human lung adenocarcinoma xenografts in nude mice in vivo .Methods The lentiviral vector expressing sur‐vivin‐siRNA was prepared .Then established human lung adenocarcinoma‐bearing nude mice model ,and divided nude mice into three groups ,including the blank control group(PC group) ,blank vector group(NC group) and experiment group(RNAi group) ,treated with physiological saline ,blank viral vector and siRNA respectively .The lentiviral vectors expressing survivin‐siRNA were locally injected in tumor issues of nude mice in the RNAi group .Then observed the curve about changes of tumor volume in different time . The expression levels of protein and mRNA were detected by using Western blot and reverse transcription polymerase chain reac‐tion(RT‐PCR) .The histogram of tumor cell cycle were examined by using flow cytometry .Results The rate of tumor inhibition of lentiviral vectors mediated survivin‐siRNA on lung adenocarcinoma in nude mice was 46 .07% .There were significant differences in tumor weight between siRNA group and NC group ,PC group(P<0 .05) ,while no statistically significant difference was found be‐tween NC group and PC group(P>0 .05) .Compared with NC group and PC group ,the expression levels of survivin mRNA and protein were decreased ,and rates of inhibition were 72 .00% and 53 .00% respectively .The percentage of G1 phase cells was in‐creased ,whereas the percentages of S phase cells was decreased .Conclusion The lentiviral vectors mediated siRNA could effective‐ly inhibit survivin gene expression on human lung adenocarcinoma xenografts in nude mice and markedly induce the apoptosis of tumor .

Chronic pancreatitis is a chronic inflammatory disease of the pancreas,whichcan lead to irreversible damage to the morphology and function of the pancreas ultimately.So the early diagnosis and treatment for chronic pancreatitis are very important.And the differentiation between mass-forming chronic pancreatitis and pancreatic cancer is also a difficult problem in clinic.MRI as a noninvasive and nonradiative examination with excellent soft tissue resolution,is very valuable for the diagnosis of chronic pancreatitis.And the combination of functional MRI and conventional MRI is very helpful for the differentiation between pancreatic carcinoma and mass-forming chronic pancreatitis,which is of great significance to the clinical management.The application and progresses of function MRI in the diagnosis and identification of chronic pancrea titis were reviewed in this article.

This paper analyzed the characteristics of pathophysiology teaching for Mongolian students.Exploration was made in aspects of designing,implementing and evaluating multilingual pathophysiology teaching mode for Mongolian students.The problems of the mode were summarized and optimization measures were proposed.

Objective To investigate quitting motivation of smoking patients with mild and moder-ate chronic obstructive pulmonary disease ( COPD) and evaluate the effects of smoking cessation methods based on cognitive intervention.Methods A total of 102 smoking patients with mild and moderate COPD from the outpatient department was divided into 4 blocks according to their intention of quitting smoking.Pa-tients in each block were randomly divided into intervention and control groups.Results ⑴All the re-spondents were at an average age of (61.3 ±12.3) years old.The sex ratio of men to women was 99∶3. The average smoking index (bag/year) in all the respondents was (39.81 ±11.53).The average daily smoking was (14.22 ±2.30) packs.The respondents with a lower degrees accounted for 55.9%and the respondents with high nicotine dependence 49.0%.A total of 39 people (38.2%) hoped to give up smok-ing, 20 people (19.6%) strongly hoped to give up smoking.⑵The main reasons for smoking included en-joyment (7.02), dependence (6.88), and calm (6.55).⑶There were statistically significant difference in the half-year continuous abstinence rates (χ2 =4.993, P =0.025) and in June quit rates (χ2 =6.668, P =0.010) between the intervention (58.82%) and control (33.33%) groups.The weight gain had sig-nificant difference between the intervention and control groups at the 3rd month ( t =2.65, P <0.01) or 6th month ( t =2.07, P <0.05) after quitting smoking.Conclusions The quitting motivation of smoking patients with mild and moderate COPD is not strong, while the smoking cessation methods based on cogni-tive intervention are effective for those patients.

Objective To investigate the distribution of Human Papillomavirus 16 (HPV16) infection and its mixed infection with other HPV subtypes in the Yunnan region. Methods 16 166 cases of women were tested using flow fluorescence Luminex technology. Results (1) HPV16 infection rate and mixed infection rate was 2.2%and 28.0%, respectively; (2) The most common type of HPV16 mixed infection was HPV52, followed by HPV33. The two kinds of mixed infection accounted for 39.8% of the total infection rate; (3) There was a significant difference between each age group of HPV16 mixed infection (Chi=26.39, <0.01) . Conclusion The HPV16 infection was mainly HPV infection in Yunnan region. HPV16 mixed infection merged mainly with HPV52 and HPV33. HPV16 mixed infection was associated with age.

Objective To investigate the expression pattern of tropomyosin receptor kinase(Trk)after focal cerebral ischemia in rat,and to explore the relationship between ischemia and the pattern of Trk expression. Methods(The model) of focal cerebral ischemia was built by middle cerebral artery occlusion.Immunocytochemistry staining was used to observe the dynamic changes of Trk expression at different time of acute stage of ischemia,and the patterns at different regions of the striate and hippocampal were detected. Results Trk immuno-positive neurons widely expressed in the normal brain.The number of Trk immuno-positive neurons was decreased abruptly in the necrosis core of the focal cerebral of ischemia,while increased obviously in the penumbra region and the DG area after 6 h of occlusion(P

Objective To study an effective treatment of severe thoracic and abdominal injury accompanied with acute respiratory distress syndromes (ARDS).Methods Emergency treatments of 32 patients with severe thoracic and abdominal trauma accompanied with ARDS were retrospectively analyzed.Results All of the 32 patients had severe thoracic and abdominal injury,ribs fracture or pulmonary contuson.Anti-shock treatment,reasonable supplemental blood volume,rational mechanical ventilation and emergency operation were performed.Twenty-six patients were cured,and 6 died,with mortality 18.75%.Conclusions Early diagnosis,timely anti-shock treatment,early treatment for thoracic and abdominal injury and correct mechanical ventilation are essential for treating thoracic and abdominal trauma accompanied by ARDS,and is also an effective method for reducing mortality.

Objective · To investigate the gender-related metabolomic differences in human saliva. Methods · The saliva metabolomic profiles of 5 male and 5 female healthy volunteers with matched age, body mass index (BMI), living and tooth-brushing condition were acquired using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry. Metabolites were identified using publicly accessible databases and further confirmed individually with standard compounds. Both multivariant and univariate statistics were conducted to find inter-gender differences.Results · Forty-eight metabolites in human saliva were identified including 13 amino acids, 6 choline metabolites, 15 carnitines, 4 sphinganine metabolites,7 lysophosphatidylcholine and 3 organic acids. Levels of phenylalanine, acetylcarnitine, propionylcarnitine, butyrylcarnitine, isobutyrylcarnitine,isovalerylcarnitine and sphinganine were higher in the saliva of females than that of males. Conclusion · Rich metabolic information present in human saliva with significant gender diffference which ought to be taken into consideration in study on the metabonomics of saliva.

Objective:To investigate the demographic characteristics and the causes for pulmonary hypertension (PH) in adult patients.Methods:A total of 2 508 adult patients diagnosed as PH,who came from the Second Xiangya Hospital of Central South University from January 2010 to December 2014,were retrospectively investigated.All subjects underwent the clinical diagnosis,or the echocardiographic diagnosis,or thetraditional hemodynamic criteria by right heart catheterization (RHC).The patient's data including hospital numbers,gender,ages,primary diseases,etc,are collected and analyzed.Results:In this study,the number of patients diagnosed as PH was increased year by year.The median age of 2 508 patients was 47 (18-93) years old,and there were 933 males (37.2％),the ratio of male to female was 1:1.69 (P＜0.05).Female was more common in Class Ⅰ PH (pulmonary arterial hypertension) and Class Ⅱ PH (pulmonary hypertension due to left heart disease)(＞70％),but there were more male patients (74.5％) in Class Ⅲ PH (pulmonary hypertension due to lung diseases and/or hypoxia).In our study,896 cases (35.73％) were the Class Ⅰ PH,1 163 cases was the Class Ⅱ PH (46.37％),411 cases was the Class Ⅲ PH (16.39％),and the Class Ⅳ PH (chronic thromboembolic pulmonary hypertension) and the Class Ⅴ PH (PH with unclear and/ or multifactorial mechanisms) were diagnosed in 32(1.27％) and 6 patients (0.24％),respectively.The diseases with largest number of patients for the top 7 primary PH were rheumatic heart disease (1 090,43.48％),congenital heart disease (692,27.60％),chronic obstructive pulmonary disease (358,14.28％),connective tissue related disease(156,6.22％),valvular heart disease (66,2.63％),idiopathic PH (46,1.83％) and pulmonary embolism (27,1.08％).Conclusion:Adult PH patients' peak incidence age is 41-50 years old.This disease is more common among women,and the Class Ⅰ/Ⅱ PH are common in women while the Class Ⅲ is more common in men.Rheumatic heart disease and congenital heart disease may be the most common cause for pulmonary hypertension in China,and chronic obstructive pulmonary disease is the most common cause for the Class Ⅲ PH,in which the patients are old.