1.Introduction of Environmental Endocrine Disruptors
Journal of Environment and Health 1993;0(01):-
The definition of environmental endocine disruptors,varieties of chemicals possible to produce endocrine disruptive effects,their possible mechanisms and adverse effects on organisms were briefly introduced in this paper.It suggested that there were many varieties of endocrine disrupting chemical existing in the human environment.The reproductive disorders reported up to date in animal maybe in human,included reduced fertility,reduced hatchability,redcued viability of offspring,impaired hormone secretion or activity and modified reproductive anatomy.Further study should be conducted and relative preventive measures should be adopted.
2.Effect of Tributyltin Chloride Exposure in Gestation on Development and Sex Hormone Level of Female Offspring Rats
Jinhe CHEN ; Jie LI ; Jiliang SI
Journal of Environment and Health 1992;0(05):-
Objective To explore the developmental toxicity of tributyltin chloride and the effects on sex hormone in female offspring rats through maternal gestation exposure. Methods Pregnant Wistar rats were randomly divided into four groups, 4 in each group. They were treated with TBTC by gavage at the dose of 0, 1, 2.5 and 5 mg/kg bw respectively from days 12-20 of gestation. 10 female offspring rats were randomly selected from each group and killed on postnatal day 70. The liver, kidney, uterus and ovary were weighed and the organ indexes were calculated. Pathological examination for liver, kidney, uterus and ovary were performed. Follicle stimulating hormone(FSH), luteotropic hormone(LH), testosterone(T) and estradiol(E2) in serum was determined by radioimmunity method. Results Increase of body weight in 2.5, 5 mg/kg bw groups significantly decreased(P
3.Effects of in Utero Di-butyltin Dilaurate Exposure in Rat on Pregnancy Outcome
Jiliang SI ; Jie LI ; Haiyan GAO
Journal of Environment and Health 1989;0(06):-
Objective To study the effects of di-butyltin dilaurate (DBTD) exposure on pregnancy outcome in Wistar rat and evaluate it's effect on sexual development of fetuses. Methods Timed pregnancy rats were treated with corn oil or DBTD (10, 20, 30 mg/kg body weight) from days 12-19 of gestation. On gestational day (GD) 20, dams were sacrificed to investigate the pregnancy outcome. Results There was a downtrend in weight of dams on GD20 as the DBTD exposure dose increased. The weight of dam's utero significantly decreased in 30 mg/kg group. A significant decrease in fetal weights was observed in all DBTD groups, and fetal sizes in 20, 30 mg/kg groups. Exposure to DBTD from GD 12-19 resulted in a distinct increase in normalized anogenital distances in female fetuses, but no effects were seen in male ones. DBTD exposure did not result in external malformations, however, delayed ossification of fetal phalanges was observed in all DBTD treated groups. Dead fetuses and absorbed embryos were observed in 20, 30 mg/kg DBTD groups. Conclusion The results of the present paper show that DBTD exposure has some adverse effects on fetal development and may exert a masculinizing effect on female fetuses.
4.Detection of Potential Microcystin-Producing Cyanobacteria in Waters With a mcyD Molecular Biomarker
Jiliang SI ; Wenrong HU ; Jie LI
Journal of Environment and Health 1992;0(02):-
Objective To develop a new method to screen the potential microcystin-producing cyanobacteria in natural waters. Methods Cyanobacteria were cultured and waters samples were analyzed by polymerase chain reaction with special primers for gene of the operon mcyD which encodes a microcystin synthetase combining conserved gene 16S rRNA of alga, subsequently, whose microcystins were detected by ELISA. Results There was a unique amplified product of approximately 870 bp in microcystin-producing blue-green alga and water samples, but no target band in non-toxic strains and samples. Conclusion It is feasible to use the molecular biological method used mcyD as the microcystin molecular biomarker to screen microcystin-producing cyanobacteria in natural water.
5.Use of microendoscopic tubular retractor system for lumbar intervertebral disc herniation: Report of 550 cases
Jie LI ; Chuanjian LIU ; Yajia SI
Chinese Journal of Minimally Invasive Surgery 2001;0(05):-
Objective To investigate the efficacy of microendoscopic tubular retractor system (METRx) in the management of lumbar intervertebral disc herniation. Methods By using the METRx system with modified instruments, the lumbar discectomy was performed in 550 cases. During the operation, patients were placed in a knee-chest sitting position, and the instrumentation was utilized into the vertebral canal through the ligamenta flava. Results The patients were followed for 6~36 months (mean, 21 months). According to the Nakai criteria, the curative outcomes were “excellent” in 478 cases (86.9%), “good” in 60 cases (10.9%),“fair” in 6 cases (1.1%), and “poor” in 6 cases (1.1%). The 6 cases with “poor” results obtained “fair” results after a re-operation of lateral recess enlargement and residual nucleus removal under microendoscope. Conclusions Lumbar discectomy with the utilization of the METRx system is minimally invasive, safe and effective.
7.Effects of Virtual Reality Rehabilitation on Balance for Patients with Parkinson's Disease
Si CHEN ; Jie LIU ; Shun LI ; Kai WANG ; Yugong HE
Chinese Journal of Rehabilitation Theory and Practice 2017;23(9):1091-1095
Objective To investigate the effects of a training program based on virtual reality on static and dynamic balance perfor-mance in patients with Parkinson's disease (PD). Methods From June, 2014 to June, 2016, 46 patients with PD were randomly divided into control group (n=23) and experimental group (n=23). The control group received routine balance training, while the experimental group re-ceived balance training of virtual reality, for six weeks. They were assessed with Unified Parkinson's Disease Rating Scale part 3 (UP-DRS3), Berg Balance Scale (BBS), TimedUp and GoTest (TUGT) and Hamilton Depression Scale (HAMD) before and after training. The envelope area, anteroposterior standard deviation (AP-SD), mediolateral standard deviation (ML-SD) of centre of pressure (COP) were also measured with posturography. Results The scores of BBS, TUGT and HAMD improved in both groups after training (t>2.657, P<0.05), and improved more in the experimental group than in the control group (t>2.426, P<0.05). The score of UPDRS3 and the parameters of pos-turography improved in the experimental group (t>2.626, P<0.05), and improved more than that in the control group (t>2.112, P<0.05). Con-clusion Virtual reality rehabilitation is more effective than routine balance training on the static and dynamic balance function in patients with PD, and may release their depression.
8.Mechanisms of gross saponins of Tribulus terrestris via activating PKCepsilon against myocardial apoptosis induced by oxidative stress.
Si-Si WANG ; Ying-Shi JI ; Hong LI ; Shi-Jie YANG
Acta Pharmaceutica Sinica 2009;44(2):134-139
This study is to observe the effect of gross saponins of Tribulus terrestris (GSTT) on protein kinase Cepsilon (PKCepsilon) and apoptosis-associated protein in the apoptosis of cultured cardiocyte apoptosis induced by hydrogen peroxide (H2O2), and to explore the mechanisms of GSTT against myocardial apoptosis. Primary cardiocytes were isolated and cultured. Myocardial apoptosis was induced by H2O2 and analyzed with flow cytometry. Protein content of phospho-PKCepsilon, Bcl-2, and Bax were detected with Western blotting analysis. Cleaved caspase-3 protein content was determined with immunocytochemical technique. After the pretreatment of 100 mg x L(-1) GSTT, compared with H2O2 group, GSTT could not only decrease the apoptotic percentage in cardiocytes damaged by H2O2 (P < 0.01), but also reduce protein contents of Bax and cleaved caspase-3 (P < 0.01), and increase protein content of phospho-PKCepsilon and Bcl-2 significantly (P < 0.01). PKC inhibitor chelerythrine (Che) could prevent partly the effect of GSTT against myocardial apoptosis (P < 0.05 and P < 0.01). Mechanisms of GSTT against myocardial apoptosis might be associated with inhibition of mitochondrial apoptosis pathway after PKCepsilon activation.
Animals
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Apoptosis
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drug effects
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Benzophenanthridines
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pharmacology
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Caspase 3
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metabolism
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Cells, Cultured
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Dose-Response Relationship, Drug
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Enzyme Activation
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Female
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Hydrogen Peroxide
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toxicity
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Male
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Myocytes, Cardiac
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cytology
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drug effects
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metabolism
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Oxidative Stress
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Phosphorylation
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Plants, Medicinal
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chemistry
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Protein Kinase C
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antagonists & inhibitors
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Protein Kinase C-epsilon
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metabolism
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Proto-Oncogene Proteins c-bcl-2
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metabolism
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Rats
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Rats, Wistar
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Saponins
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administration & dosage
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isolation & purification
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pharmacology
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Tribulus
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chemistry
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bcl-2-Associated X Protein
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metabolism
9.Advance in re-do pyeloplasty for the management of recurrent ureteropelvic junction obstruction after surgery.
Sheng Wei XIONG ; Jie WANG ; Wei Jie ZHU ; Si Da CHENG ; Lei ZHANG ; Xue Song LI ; Li Qun ZHOU
Journal of Peking University(Health Sciences) 2020;52(4):794-798
Ureteropelvic junction obstruction (UPJO) is characterized by decreased flow of urine down the ureter and increased fluid pressure inside the kidney. Open pyeloplasty had been regarded as the standard management of UPJO for a long time. Laparoscopic pyeloplasty reports high success rates, for both retroperitoneal and transperitoneal approaches, which are comparable to those of open pyeloplasty. However, open and laparoscopic pyeloplasty have yielded disappointing failure rates of 2.5%-10%. The main causes for recurrent UPJO are severe peripelvic and periureteric fibrosis due to urinary extravasation, ureteral ischemia, and inadequate hemostasis. In addition, failing to diagnose lower pole crossing vessels before or during the primary procedure is also responsible for recurrent UPJO. In addition, poor preoperative split renal function, hydronephrosis, presence of renal stones, patient age, diabetes, prior endopyelotomy history, and retrograde pyelography history were considered as predictors of pyeloplasty failure. The failure is usually defined by persistent pain, persistent radiographic obstruction (infection or stones), continued decline in split renal function, or a combination of the above. And the failure of pye-loplasty often occurs in the first 2 years after the surgery. The available options for managing recurrent UPJO with a salvageable renal unit include endopyelotomy, re-do pyeloplasty, stent implantation, percutaneous nephrostomy, ureterocalicostomy, and nephrectomy. Re-do pyeloplasty has such merits as high successful rates and rare complications, compared with endopyelotomy or ureterocalicostomy. And some investigators think that re-do pyeloplasty should be regarded as the gold standard for secondary therapy if feasible. Open pyeloplasty can enlarge the operating field, facilitate the exposure of the ureteropelvic junction, reduce the difficulty of operation, and thus reduce the occurrence of complications. There are no significant differences among the success rates of re-do pyeloplasty under open approach, traditional laparoscopy and robot-assisted laparoscopy, according to previous reports. However, traditional laparoscopic and robot-assisted pyeloplasty give advantages of cosmetology, small trauma, less postoperative pain, speedy recovery and shorter hospitalization, fewer complications and lower recurrent rates. If the primary pyeloplasty is an open operation in retroperitoneal approach, the traditional laparoscopic and robotic operation with retroperitoneal approach should be considered for secondary repair. The cause of recurrent UPJO should be evaluated before surgery and identified intraoperatively to minimize the possibility of recurrence.
Humans
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Hydronephrosis
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Kidney Pelvis
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Laparoscopy
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Ureter
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Ureteral Obstruction/surgery*
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Urologic Surgical Procedures
10. 3.0T MRI multi-echo dixon technique in quantitative analysis of liver fat in patients with nonalcoholic fatty liver disease
Academic Journal of Second Military Medical University 2016;37(9):1088-1094
Objective To investigate the feasibility of using 3. 0T MRI multi-echo (ME) Dixon technique for liver fat quantification in patients with nonalcoholic faity liver disease (NAFLD). Methods Twenty volunteers were enrolled in this prospective study, and they included 16 NAFLD patients (12 males and 4 females) and 4 healthy volunteers (3 males and one female). All volunteers were examined by routine liver MRI. VIE Dixon technique and single-voxel MRS. After examination. the fat fraction (FF) value of Screening Dixon and the FF value of single-voxel MRS with Histo sequence were recorded, and the FF values of 3 regions of interest (ROIs) in the fat fraction map of ME Dixon were manually measured Finally, the FF values were analyzed with Spearman correlation analysis. Results The Screening Dixon FF value was positively correlated with the FF values of three ROIs of ME Dixon (r = 0. 842. 0. 959. and 0. 945 respectively, all P<0. 001). Similarly, positive correlation was also found for the FF values of Screening Dixon and 3 ROIs of ME Dixon with the FF value of single-voxel MRS with Histo sequence(r=0. 971, 0. 842, 0. 959. and 0. 945 respectively,all P<0. 001). Conclusion h is feasible to use 3. 0T MRI ME Dixon for evaluating liver fat content in patients with NAFLD. and iis results are positively correlated with those of single-voxel MRS. It is valuable for the diagnosis, follow-up and intervention of NAFLD patients.