In case of limbitis, cytokines secreted by PMNs might inhibit the function of corneal epithelial stem cells located at the limbal basal cell layer. The purpose of this study was to examine whether PMNs factor affected stem cells (SC). PMNs obtained from the peritoneal cavity of rabbits after 0.2% glycogen stimulation incubated in DMEM at 37 degrees C for 24 hours. PMNs factor was obtained from the medium using dialysis. The death or inhibition of growth of rabbit corneal epithelial cells (CE) by PMNs factor was studied in vitro. PMNs factor and PBS as a control were injected into the limbal region for clinical evaluation and histopathologic study. ID 50 of PMNs factor for cultured CE was equal to the amount of polypeptide produced by 1.29 x 0 (7) PMNs. Cultured rabbit CE shrank and began to die 24 hours after exposure to PMNs factor. Abnormal findings were observed 5 days and 8 months after injection of PMNs factor at the limbus by light and electron microscopy. Clouding, defect, and vascularization of CE were noted several months after injection of PMNs factor. PMNs in limbitis may damage corneal epithelial stem cells and cause epithelial instability of the cornea.
Cornea ; Cytokines ; Dialysis ; Epithelial Cells ; Glycogen ; Microscopy, Electron ; Neutrophils* ; Peritoneal Cavity ; Rabbits ; Stem Cells*

Several symptoms of dry eye in the patients who underwent cataract surgery are related to the stability of precorneal tear film layer. We studied the change of the lipid layer pattern in tear film after cataract surgery. Ninety-eight eyes of 87 patients were evaluated for the lipid layer patterns before operation and at two months after operation. Thirty-two eyes(32.7%) among 98 eyes had the change of the pattern of lipid layer. The change was more marked in the eyes of color fringe pattern preoperatively than others(p=0.000). The rate of change was not significantly different between the superior limbal incision group and temporal clear corneal incision group, and between the groups according to the number of sutures(p>0.05). The eyes which had had the dry eye symptoms developed more change of the lipid layer pattern(p=0.006). After cataract surgery, the change of the lipid layer pattern got more marked especially in the eyes which had had color fringe pattern before surgery. In the eyes which had had the dry eye symptoms, however, no difference was shown by the incisional site and by the number of sutures.
Cataract* ; Humans ; Sutures ; Tears*

1,072 cases of ocular injuries, including 271 cases of in-patients, who visited department of ophthalmology of Chungnam National University Hospital from January 1,1981 to December 31, 1985, were clinically analyzed. The results were as follows: 1. The incidence of ocular injuries was 8.1% of all eye patients and 16.0% of all patients admitted to this ophthalmologic department. 2. The incidence was more common in male(82.0%) and in the age of 3rd to 4th decades(53.7%). 3. Monocular injuries accounted for 90.4% of ocular injuries. There was no significant difference in the incidence between the right and left eye. 4. Tho ocular injuries were more common in the spring(27.4%), but in children the incidence was higher during the vacation. 5. The patients who visited this hospital within 24 hours after injury accounted for 89.8%. 6. The most common cause of ocular injuries was fist or finger(15.9%), followed by iron products(12.6%) and traffic accident(10.9%), but the injuries by iron products(32.1%) were most common in the admitted patients. 7. The most common ocular injury was eyelid laceration (15.3%), followed by subconjunctival hemorrhage(12.5%) and conjunctival foreign body(8.6%). In the cases of in-patients, corneal laceration(21.2%) was most common, followed by corneoscleral laceration(14.9%) and lens perforation(10.2%). The corneal perforation was 52.1% of all perforating eye injuries. 8. Surgical procedure included corneal suture(21.1%), lensectomy(12.5%), enucleation or evisceration(11.2%), and others. 9. Visual acuity was improved in most cases by treatment, but the corrected vision after treatment was less than 0.1 in 33.2%, which was mainly due to the perforating eye injuries. 10. The most common complication of ocular injuries after treatment was corneal opacity(36.3%), followed by secondary glaucoma(14.6%) and traumatic cataract(6.4%).
Child ; Chungcheongnam-do ; Corneal Perforation ; Eye Injuries ; Eyelids ; Humans ; Incidence ; Iron ; Lacerations ; Ophthalmology ; Visual Acuity

PURPOSE: Several sets of criteria have been proposed and used by different authors, but the patients with Sjoren's syndrome have been missed at diagnosis or incorrectly classified due both to its great variability at presentation and to the lack of commonly accepted diagnostic criteria. We proposed a criteria for Sjoren's syndrome and attempted to utilize this criteria for diagnosis and classification of dry eye patients who were suspected of Sjoren's syndrome. METHODS: We proposed and applied a set of criteria for Sjoren's syndrome, which included clinical, histopathological and laboratory features as well as exclusions for the diagnosis, to 6 dry eye patients who were clinically at Chungnam National University Hospital from January 1992 to December 1996. RESULTS: Two of the 6 patients who were clinically suspected of Sjoren's syndrome had satisfactory histopathological results. This two patients were diagnosed as primary Sjoren's syndrome. CONCLUSIONS: Our criteria would be helpful to the diagnosis and classification of the patients with Sjoren's syndrome, who were previously diagnosed as dry eye syndrome.
Chungcheongnam-do ; Classification ; Diagnosis ; Dry Eye Syndromes ; Humans

We investigated mean keratometric vale(K) after penetrating keratoplasty(PKP) and analyzed the factors which affected the postoperative mean K. We obtained mean K at postoperative 3 months in the 65 eyes of 65 patients which PKP of PKP combined with intraocular lens(IOL) implantation was done, and analyzed those values according to sex, age, preoperative diagnosis, preoperative corneal neovascularization, methods of trephination, methods of suture and methods of operation. The postoperative mean K of all patients was 42.83 diopters(D) and 43.32D in the recipient cornea without neovascularizaion, 42.51D with neovascularization, 42.72D in the cases of hand-held trephination and 43.27D in Hessvurg-Varron trephination, which had no statistical significant difference between K and sex, age, preoperative diagnosis, methods of suture, and methods of operation. This results will be helpful to predict the change of K ofter PKP, and give a better refractive results to use the operator's own mean K for the IOL calculation in the case of PKP comvined with IOL implantation.
Cornea ; Corneal Neovascularization ; Diagnosis ; Humans ; Keratoplasty, Penetrating* ; Sutures ; Trephining

To investigate the accuracy of ultrasonic measurement of axial length in pseudophakia, we measured axial length by ultrasonic scan at pre-and post-operatively.One hundred nine eyes of 97 patients were divided according to the type of intraocular lens.We compared pre-and post-operative axial lengths with Ocuscan[Alcon, U.S.A.]. The postoperative axial lengths were measured in phakic, aphakic, and pseudophakic mode.In pseudophakic mode, the velocity in IOL was set at the rate of 980, 2200 and 2718m/sec for silicone, acrylic, and PMMA lens respectively. The postoperative axial lengths in pseudophakic mode were closest to the preoperative values in all groups. In conclusion, we can get the closest values to preoperative axial lengths by setting the velocity in pseudophakic mode at the rate of 980, 2200 and 2718m/sec for silicone, acrylic and PMMA lens, respectively.
Humans ; Lenses, Intraocular ; Polymethyl Methacrylate ; Pseudophakia* ; Silicones ; Ultrasonics*

STUDY DESIGN: In vitro experimental study. OBJECTIVES: To examine the cellular proliferation, synthetic activity and phenotypical expression of intervertebral disc (IVD) cells seeded on types I and II atelocollagen scaffolds, with the stimulation of TGF-beta1 and BMP-2. SUMMARY OF LITERATURE REVIEW: Recently, tissue engineering is regarded as a new experimental technique for the biological treatment of degenerative IVD diseases, and has been highlighted as a promising technique for the regeneration of tissues and organs in the human body. Research on cell transplantation in artificial scaffolds has provided that the conditions for tissue engineering have to be equilibrated, including the cell viability and proliferation, maintenance of characteristic phenotype, suitable scaffolds in organisms and biologically stimulated growth factor. MATERIAL AND METHOD: Lumbar IVD cells were harvested from 10 New Zealand white rabbits, with the nucleus pulposus cells isolated by sequential enzymatic digestion. Each of 1% types I and II atelocollagen dispersions were poured into a 96-well plate (diameter 5 mm), frozen at -70 degrees C, and then lyophilized at -50 degrees C. Fabricated porous collagen matrices were made using the cross-linking method. Cell suspensions were imbibed by surface tension into a scaffold consisting of atelocollagen. The cell cultured scaffolds were then treated with TGF-beta1 (10 ng/ml) or BMP-2 (100 ng/ml) or both. After 1 and 2 week culture periods, the DNA synthesis was measured by [3H] thymidine incorporation, and newly synthesized proteoglycan by incorporation of [35S] sulphate. Reverse transcription-polymerase chain reactions for the mRNA expressions of type I and II collagen, aggrecan and osteocalcin were performed. The inner morphology of the scaffolds was determined by scanning electron microscopy (SEM). RESULTS: The IVD cultures in collagen type II with TGF-beta1 demonstrated an increase in proteoglycan synthesis and up regulation of aggrecan and types I and II collagen mRNA expressions compared to the control. IVD cultures in the type I atelocollagen scaffold with growth factors exhibited an increase in DNA synthesis and up regulation of the type II atelocollagen mRNA expression. With all combinations of growth factor, the IVD cultures in types I and II atelocollagen scaffolds showed no up regulation of the osteocalcin mRNA expression. Furthermore, there was no synergistic effect of TGF-beta1 and BMP-2 in the matrix synthesis or for the mRNA expression of the matrix components. CONCLUSIONS: Nucleus pulposus cells from rabbit were viable in atelocollagen types I and II atelocollagen scaffolds. The type I atelocollagen scaffold was suitable for cell proliferation, but the type II atelocollagen scaffold was more suitable for extracellular matrix synthesis. The IVD cells in both scaffolds were biologically responsive to growth factors. Taken together, nucleus pulposus cells in atelocollagen scaffolds, with anabolic growth factors, provide a mechanism for tissue engineering of IVD cells.
Aggrecans ; Cell Proliferation ; Cell Survival ; Cell Transplantation ; Collagen ; Collagen Type II ; Digestion ; DNA ; Extracellular Matrix ; Human Body ; Intercellular Signaling Peptides and Proteins* ; Intervertebral Disc* ; Microscopy, Electron, Scanning ; Osteocalcin ; Phenotype ; Proteoglycans ; Rabbits ; Regeneration ; RNA, Messenger ; Surface Tension ; Suspensions ; Thymidine ; Tissue Engineering ; Transforming Growth Factor beta1 ; Transplants ; Up-Regulation

STUDY DESIGN: In vitro experimental study. OBJECTIVES: To examine the cellular proliferation, synthetic activity and phenotypical expression of intervertebral disc (IVD) cells seeded on types I and II atelocollagen scaffolds, with the stimulation of TGF-beta1 and BMP-2. SUMMARY OF LITERATURE REVIEW: Recently, tissue engineering is regarded as a new experimental technique for the biological treatment of degenerative IVD diseases, and has been highlighted as a promising technique for the regeneration of tissues and organs in the human body. Research on cell transplantation in artificial scaffolds has provided that the conditions for tissue engineering have to be equilibrated, including the cell viability and proliferation, maintenance of characteristic phenotype, suitable scaffolds in organisms and biologically stimulated growth factor. MATERIAL AND METHOD: Lumbar IVD cells were harvested from 10 New Zealand white rabbits, with the nucleus pulposus cells isolated by sequential enzymatic digestion. Each of 1% types I and II atelocollagen dispersions were poured into a 96-well plate (diameter 5 mm), frozen at -70 degrees C, and then lyophilized at -50 degrees C. Fabricated porous collagen matrices were made using the cross-linking method. Cell suspensions were imbibed by surface tension into a scaffold consisting of atelocollagen. The cell cultured scaffolds were then treated with TGF-beta1 (10 ng/ml) or BMP-2 (100 ng/ml) or both. After 1 and 2 week culture periods, the DNA synthesis was measured by [3H] thymidine incorporation, and newly synthesized proteoglycan by incorporation of [35S] sulphate. Reverse transcription-polymerase chain reactions for the mRNA expressions of type I and II collagen, aggrecan and osteocalcin were performed. The inner morphology of the scaffolds was determined by scanning electron microscopy (SEM). RESULTS: The IVD cultures in collagen type II with TGF-beta1 demonstrated an increase in proteoglycan synthesis and up regulation of aggrecan and types I and II collagen mRNA expressions compared to the control. IVD cultures in the type I atelocollagen scaffold with growth factors exhibited an increase in DNA synthesis and up regulation of the type II atelocollagen mRNA expression. With all combinations of growth factor, the IVD cultures in types I and II atelocollagen scaffolds showed no up regulation of the osteocalcin mRNA expression. Furthermore, there was no synergistic effect of TGF-beta1 and BMP-2 in the matrix synthesis or for the mRNA expression of the matrix components. CONCLUSIONS: Nucleus pulposus cells from rabbit were viable in atelocollagen types I and II atelocollagen scaffolds. The type I atelocollagen scaffold was suitable for cell proliferation, but the type II atelocollagen scaffold was more suitable for extracellular matrix synthesis. The IVD cells in both scaffolds were biologically responsive to growth factors. Taken together, nucleus pulposus cells in atelocollagen scaffolds, with anabolic growth factors, provide a mechanism for tissue engineering of IVD cells.
Aggrecans ; Cell Proliferation ; Cell Survival ; Cell Transplantation ; Collagen ; Collagen Type II ; Digestion ; DNA ; Extracellular Matrix ; Human Body ; Intercellular Signaling Peptides and Proteins* ; Intervertebral Disc* ; Microscopy, Electron, Scanning ; Osteocalcin ; Phenotype ; Proteoglycans ; Rabbits ; Regeneration ; RNA, Messenger ; Surface Tension ; Suspensions ; Thymidine ; Tissue Engineering ; Transforming Growth Factor beta1 ; Transplants ; Up-Regulation

Mazabraud syndrome (MS) is a rare and sporadic disorder. It is mainly characterized by fibrous dysplasia (FD) of single or multiple bones and intramuscular myxomas (IM). Data on the prevalence since it was first reported, clinical features, and prognosis are extremely scarce. We report a case of a 59-year-old woman with IM and polyostotic FD. She also had multiple cafe’-au-lait spots suggestive of McCune-Albright syndrome (MAS). On magnetic resonance imaging, there are masses with well-defined heterogeneous enhancement, accompanied by an inner cyst in the vastus lateralis muscle and femur. These radiological results are identical to those of FD. After surgical intervention with excision of intramuscular soft-tissue mass, a diagnosis of IM of MS was confirmed. Given that cafe’-au-lait spots also appeared, the patient was diagnosed with a variant of MS with some of the clinical characteristics of MAS.

PURPOSE: To compare surgically induced astigmatism and influences on the corneal endothelium in phacoemulsification by Ozil(R) and Hyperpulse mode of Infiniti(R) using 2 different corneal incision lengths of 2.2 mm and 2.8 mm. METHODS: The patients were grouped by the mode of phacoemulsification and incision size as follows: Ozil(R) mode with 2.2 mm incision group (n = 29), and 2.8 mm incision group (n = 30); Hyperpulse mode with 2.2 mm incision group (n = 29), and 2.8 mm incision group (n = 30). The surgically induced astigmatism, central corneal thickness and endothelial cell density were measured up to 2 months after surgery and the efficiency of 2 modes compared by measuring average phacoemulsification times. RESULTS: There was no significant difference in the surgically induced astigmatism, central corneal thickness, endothelial cell density, best corrected visual acuity and average phaco power among 4 groups. The cumulated dissipated energy (CDE) using Ozil(R) mode was lower than Hyperpulse mode in the group of nuclear sclerosis grade 1 and 2. CONCLUSIONS: Two different modes of phacoemulsification using Ozil(R) and Hyperpulse mode, and different incision sizes of 2.2 mm and 2.8 mm in Infiniti(R) showed no significant difference in the endothelial cell density or the surgically induced astigmatism up to 2 months after surgery. Phacoemulsification using Ozil(R) mode is considered more efficient in low grade nuclear sclerosis cataract.
Astigmatism ; Cataract ; Endothelial Cells ; Endothelium, Corneal ; Humans ; Phacoemulsification ; Sclerosis ; Visual Acuity