1.UPLC-TOF/MS based chemical profiling approach to evaluate toxicity-attenuated chemical composition in combination of ginseng and radix aconiti praeparata.
Zeng-Chun MA ; Si-Si ZHOU ; Qian-De LIANG ; Chao HUO ; Yu-Guang WANG ; Hong-Ling TAN ; Cheng-Rong XIAO ; Yue GAO
Acta Pharmaceutica Sinica 2011;46(12):1488-1492
In the present study, an ultra performance liquid chromatography coupled with time-of-fight mass spectrometry (UPLC-TOF/MS) based chemical profiling approach was used to evaluate chemical constitution between co-decoction and mixed decoction of ginseng and Radix Aconiti Praeparata. Two different kinds of decoctions, namely co-decoction of ginseng and Radix Aconiti Praeparata: water extract of mixed two herbs, and mixed decoction of ginseng and Radix Aconiti Praeparata: mixed water extract of each individual herbs, were prepared. Batches of these two kinds of decoction samples were subjected to UPLC-TOF/MS analysis. The datasets of t(R) m/z pairs, ion intensities and sample codes were processed with supervised partial least squared discriminant analysis (OPLS-DA) to holistically compare the difference between these two decoction samples. Significant difference between the two decoction samples was showed in the results of positive ion mode. The contents of hypaconitine and deoxyaconitine decreased, while that of benzoylmesaconine, benzoylhypaconine and dehydrated benzoylmesaconine increased in the samples of co-decoction of ginseng and Radix Aconiti Praeparata. The content of diester-diterpenoid alkaloids decreased, while that of monoester-diterpenoid alkaloids increased, which is probably the basis of toxicity-attenuated action when combined ginseng with Radix Aconiti Praeparata.
Aconitine
;
analogs & derivatives
;
analysis
;
Aconitum
;
chemistry
;
Alkaloids
;
analysis
;
Chromatography, High Pressure Liquid
;
methods
;
Drug Combinations
;
Drugs, Chinese Herbal
;
chemistry
;
Panax
;
chemistry
;
Plants, Medicinal
;
chemistry
;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
;
methods
2.Effect of the Chinese Herbal Medicinal Ingredients in Huoxiang Zhengqi Liquid on Alcohol Metabolism in Rats.
Si Huo ZENG ; Jun Xing OU ; Juan Hua GU ; Kai Run YANG ; Rui WANG ; Zhi LI ; Shu Hua LI ; Rui Lin ZHANG
Journal of Forensic Medicine 2016;32(6):424-427
OBJECTIVES:
To study the changes of alcohol content and pharmacokinetic parameter in rats after taking Huoxiang Zhengqi liquid.
METHODS:
The rats were randomly divided into three groups and given with white alcohol at the dose of 3.0 mL/kg, low-dose and high-dose Chinese medicine liquor, respectively. The blood was collected before administration and 5 min, 10 min, 15 min, 30 min, 1 h, 2 h, 3 h, 4 h, 5 h, 6 h and 8 h after administration by cutting rats' tails. The concentrations of alcohol in blood were detected by headspace-gas chromatography method. The main pharmacokinetic parameters were calculated by DAS 2.0, and then analyzed by SPSS 17.0.
RESULTS:
The difference of maximum blood concentrations between high-dose Chinese medicine alcohol group and white alcohol group was statistically significant (P<0.05). There was no significant difference in other pharmacokinetic parameters among three groups (P>0.05).
CONCLUSIONS
The Chinese herbal medicinal ingredients in the Huoxiang Zhengqi liquid has no effect on the metabolism and elimination of ethanol in rats. The research provides useful reference for the qualitative assessment and processing of traffic accident cases involved in Huoxiang Zhengqi liquid and the studies related to drug-interaction.
Animals
;
Drugs, Chinese Herbal/pharmacology*
;
Ethanol/pharmacokinetics*
;
Rats