OBJECTIVETo observe the ultrastructure of bone marrow stromal cells (BMSCs) cultured in coralline hydroxyapatite (CHA) and evaluate their biocompatibility.

METHODSBMSCs isolated from dogs were cultured with CHA as the scaffold, and the morphologies of the cells were observed with phase-contrast microscope and scanning electron microscope.

RESULTS AND CONCLUSIONBMSCs grew well with good attachment to the CHA scaffold and performed normal function, demonstrating CHA as one of useful biocarrier materials for bone tissue engineering.

Animals ; Bone Marrow Cells ; cytology ; ultrastructure ; Bone Substitutes ; chemistry ; Cell Culture Techniques ; Cells, Cultured ; Ceramics ; chemistry ; Dogs ; Hydroxyapatites ; chemistry ; Male ; Microscopy, Electron, Scanning ; Microscopy, Phase-Contrast ; Stromal Cells ; cytology ; ultrastructure ; Tissue Engineering ; methods ; Tissue Scaffolds ; chemistry