1.Analysis of the correlation of MGMT and XRCC1 gene expression with the pathogenesis of glioma and its mechanism analysis
Shuzi GAO ; Dezhu AN ; Xinfeng YI ; Jiayan LI ; Hong BAO
Journal of Chinese Physician 2018;20(7):1013-1016
Objective To investigate the relationship between the expression of 06-methyl guanine DNA methyltransferase (MGMT),X-ray repair cross complementation gene 1 (XRCC1) and the incidence of glioma.Methods From February 2015 to September 2017,53 glioma patients (glioma group) in our hospital were enrolled in the study.50 patients with hypertensive intracerebral hemorrhage were selected as control A group,and 106 healthy volunteers as control B group.Immunohistochemical staining was used to detect the expression of MGMT and XRCC1 in brain tissue of glioma group and control A group,and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were used to detect the polymorphism of MGMT and XRCC1 gene in glioma group and control B group.Results The positive expression rates of MGMT and XRCC1 in the tissues of brain glioma were 47.17% and 39.62%,respectively,which were significantly higher than those in the control A group (P < 0.05).There was no significant difference in the positive expression rate of MGMT and XRCC1 in patients with grade Ⅰ,,Ⅲ and Ⅳ (P > 0.05);There was no correlation between the expression of MGMT and XRCC1 in glioma tissues (rs =0.162,P > 0.05);The proportion of XRCC1 genotype AG + GG in brain glioma group was 58.49%,which was significantly higher than that of control B group (P < 0.05);The proportion of MGMT genotype LP + PP in brain glioma group was 28.30%,which was significantly higher than that of control B group (P < 0.05).Conclusions MGMT and XRCC1 are increased significantly in glioma brain tissues,but not correlatedwith pathological grades;The polymorphism of MGMT and XRCC1 genes may be related to the susceptibilityof gliomas.
2.Current status and prospect of biomarker research for schizophrenia
Mengyuan ZHU ; Qing CHEN ; Dan LI ; Mengxia WANG ; Renyu WANG ; Yuxin ZHU ; Weifeng JIN ; Shuzi CHEN ; Ping LI ; Zhenhua LI ; Peijun MA ; Shuai LIU ; Qiong GAO ; Xiaoyan LOU ; Jie XU ; Lili ZHU ; Ling ZHAO ; Kangyi LIANG ; Jinghong CHEN ; Xunjia CHENG ; Ke DONG ; Xiaokui GUO ; Qingtian LI ; Yun SHI ; Junyu SUN ; Huabin XU ; Ping LIN
Chinese Journal of Laboratory Medicine 2022;45(11):1191-1196
Schizophrenia is a serious mental disease. The diagnosis of schizophrenia so far relies heavily on subjective evidence, including self-reported experiences by patients, manifestations described by relatives, and abnormal behaviors assessed by psychiatrists. The diagnosis, monitoring of the disease progression and therapy efficacy assessment are challenging due to the lack of established laboratory biomarkers. Based on the current literature, clinical consensus, guidelines, and expert recommendations, this review highlighted evidence-based potential laboratory biomarkers for the diagnosis of schizophrenia, including genetic biomarkers, neurotransmitters, neurodevelopmental-related proteins, and intestinal flora, and discussed the potential future directions for the application of these biomarkers in this field, aiming to provide an objective basis for the use of these biomarkers in the early and accurate diagnosis, treatment, and prognosis and rehabilitation assessment of schizophrenia.