The amino acid mixture solution was prepared from bean and maize embryo dregs by microbial degradation. The solution contains eighteen kinds of amino acid. The eight kinds of essential amino acid was 41.5 per cent of the total amino acid mixture solution and the resultant ratio of amino acid, produce artio, utilization ratio of raw material protein and resultant ratio of amino acid nitrogen were 40, 18, 87.2 and 52.6 per cent respectively.The experimental animals were given basic food and amino acid mixture solution as a substitute for drinking water. The experiment was divided into four groups i.e. A (100% instead of the water), B(50%), C (33%), D (100% tap water). The growth rate, swimming time and antianoxic survival time of all test animals were investigated. The result showed that amino acid mixture solution from bean and maize embryo dregs might play a benefitial roles in animal growth rate, antifatigue, maintaning strength of anti-anoxia and increasing stamina.

To compare the activity of RGD-TRAIL in different expression systems, RGD-TRAIL in both Escherichia coli (E.coli) and Pichia pastoris was constructed and expressed. In vitro activity of RGD-TRAIL from Pichia pastoris expression system was also analyzed. Genetic engineering techniques were used to construct recombinant plasmid pET30-rgd-trail and pHBM-rgd-trail. The recombinant protein RGD-TRAIL was purified with Ni ion affinity chromatography after induction. MTT assay, ELISA, scratch wound healing, transwell migration assay and Hoechst 33342 staining were performed to detect the effects of RGD-TRAIL on proliferation, binding activity, migration and apoptosis. The expression of apoptosis-associated proteins was detected by Western blotting. Recombinant protein RGD-TRAIL was successfully expressed in a form of inclusion body in E.coli, while expressed secretorily in Pichia pastoris. It possessed more potent cytotoxicity than RGD-TRAIL in E.coli by MTT assay. The RGD-TRAIL expressed by Pichia pastoris showed powerful binding affinity with cancer cells expressing α(v), DR4, DR5 and highly potent cytotoxicity through inducing apoptosis of cancer cells. Nuclear fragmentation was examined by Hoechst 33342 staining. Cleaved PARP and caspase-3 were also detected after incubation with RGD-TRAIL. Additionally, RGD-TRAIL inhibited migration significantly in A549 and HT1080 cells. The results demonstrate that Pichia pastoris expression system is more suitable for the recombinant protein RGD-TRAIL. Its binding affinity and antitumor activity might make RGD-TRAIL a promising candidate for cancer therapy.

Objective To know about the status quo and influencing factors of undergraduate nurses' psychological empowerment at present,and provide a theoretical basis for nursing administrators so as to take measures to raise the level of psychological empowerment of undergraduate nurses.Methods General information questionnaire for nurses,Psychological Empowerment Scale were used to survey 207 undergraduate nurses in 3 first class teaching hospital of Dalian City.Results Undergraduate nurses' psychological empowerment score was (43.890±6.515) points,four dimensions whose scores from high to low were self-efficacy,job significance,autonomy and job impact.There was statistical difference in terms of ages,working length,working positions,professional titles,working purposes and training opportunities.Among them,working position and working purpose were the most important influencing factors of psychological empowerment.Conclusions Psychological empowerment of undergraduate nurses needs to be improved,managers should pay attention to the role of psychological empowerment,and actively cultivate and correctly guide undergraduate nurses in order to stimulate their potential in work,and finally,promote the sustainable development of a solid nursing career.

ObjectiveTo explore the diagnosis and treatment of incomplete Kawasaki disease.MethodsA retrospective analysis was applied in 39 children patients clinically diagnosed as Kawasaki disease.ResultsThirtynine cases were found to have a high temperature over 5 days( 100％ ),30 patients with bilateral conjunctival congestion (76.9％),28 cases with cherry pink and chapped lips( 71.8％ ),21 patients with erythema multiforme and erythra(53.8％ ),20 cases with hand-foot scleredema(51.3％ )and 10 patients with cervical lymphatic enlargement (25.6％).In addition to the high temperature,22 cases were found to have three classic clinical diagnostic characterstics(56.4％ ),and 17 patients with two classic characterstics(43.6％ ).During the course of disease,C-reactve protein was observed to increase,and erythrocyte sedimentation rate speed up significantly.Intravenous infusion of gamma globulin and oral administration of aspirin was confirmed to be effective for incomplete Kawasaki disease.ConclusionThe careful observation and complete clinical examination was conducive to early diagnosis of incomplete Kawasaki disease instead of misjudge and missed diagnosis.

As an important component of hospital information system (HIS), outpatient information management system (OIMS) should be emphasized on during the process of hospital digitalization. This paper summarizes the main contents and functions of OIMS and discusses the most important sub-system of OIMS's, outpatient doctor workstation, on its case history, examination/Lab and prescription. The generalpurpose card system for outpatient service will be set up on the basis of computerized OIMS, and thus the problems of outpatient can be eliminated completely.

Objective To retrospectively analyze the expression of SARS-CoV (IgG) in the serum of hospitalized children who were not suffering from SARS, and retrospectively evaluate the difference of generation of the antibody between the children and adults. Methods Serum samples were collected from 85 hospitalized children, 500 health-check adults, 976 hospitalized adults, and 156 adults recovered from severe acute respiratory syndrome (SARS), and SARS-CoV (IgG) in the specimens was determined by ELISA and biologic chip. Results The level of serum SARS-CoV (IgG) in childhood patients was significantly higher than that in adult patients (17.6% vs 6.3%, P

90 bpm and nicardipine 10?g?kg-1 when MAP increased by 20% of the baseline value. Radial artery was cannulated for continuous BP monitoring and blood sampling. MAP, ECG, HR, SpO2 , PET CO2 and body temperature were continuously monitored during anesthesia. Blood samples were taken before induction of anesthesia (T1), when dura was cut (T2), when aneurysm was clipped (T3 ) and 30 min after clipping (T4 ) for determination of plasma concentrations of AT- Ⅱ , ET and CGRP by radioimmuno-assay.Results Plasma concentration of AT- Ⅱ and HR did not change significantly through out the study. Plasma CGRP concentration and MAP decreased significantly during operation at T2-4 as compared to the baseline values (T1 ) . Plasma ET concentration increased significantly at clipping (T3 ) as compared to that at T2. Conclusion The action of vaso-constrictors (AT- Ⅱ , ET) predominates over that of vaso-dilator during operation. It is important to prevent acute cerebral vasospasm during intracranial aneurysm clipping.

Cerebral topographic map (CTM) and electroencephalogram (EEG) can evaluate the functional activity of brain, and the purpose of this study was to objectively compare the effect of intravenous anesthetics on brain. Forty-five patients, ASA grade Ⅰ-Ⅱ, undergoing neurosurgery,were randomly divided into three groups after administration of Innovar 2 ml, and then equivalent effective dose of propofol(2～2.5 mg/kg)(Ⅰ),sodium thiopental (4～6mg/kg)(Ⅰ) or gama hydroxybutyrate sodium (70～80mg/kg)(Ⅲ) was intravenously given respectively. All patients were monitored continuously during induction. The high frequent excitative activities of ? rhythm appeared immediately after each anesthestic was injected. During the most effective period: in group Ⅰ, there were no ? rhythm in EEG and obvious power change on CTM; in group Ⅱ irregular ? rhythm took place in EEG occasionally and great increase in top power on CTM occured, which showed evident elevation compared with group Ⅰ (P

Objective: To observe the effects of total intravenous anesthesia(TIVA)and spontaneous respiration on brain oxygen balance status in patients undergoing brain stem neoplasm resection. Method: Thirty patients,ASA grade Ⅰ-Ⅱ, received endotracheal intubation with spontaneous respiration and TIVA. TIVA was maintained with intravenous 0.2% lidocaine 2-3mg?kg~(-1). h~(-1),0.4% propofol 4-6mg?kg~(-1),h~(-1)and 1% ketamine 1-1.5mg?kg~(-1). h~(-1). Arterial and jugular bulb blood samples were taken before and after induction,sawing skull and removing trmor seperately to analyse blood gas. According to Fick principle, the level of CaO_2,CjvO_2 and C (a-jv)O_2 were calculated. Result:The hymodynamics, SaO_2 and CaO_2 remained stable during whole procedure. In comparison with the baselines. respiratory rate, minute volume, arterial blood pH and C(a-jv)O_2 were decreased, and PaCO_2.SjvO_2, and CjvO_2 were increased significantly (P

Objective:To study the effects of periodontal treatment on the levels of serum lipids and lipoprotein associated phospho-lipase A2(LP-PLA2)in patients with chronic periodontitis(CP)and metabolic syndrome(MS).Methods:52 cases of CP with MS were included,the blood lipid levels,clinical periodontal indexes and white blood cell count(WBC)were detected before and 3 months after treatment.Results:Before and after periodontal therapy the levels of AL(mm)were 5.02 ±0.68 and 3.61 ±0.43(P<0.01),PD(mm)4.07 ±0.46 and 2.52 ±0.39(P<0.01),BOP positive loci(%)92.13 ±6.98 and 37.41 ±8.19(P<0.01), PLI 1.38 ±0.29 and 0.89 ±0.27(P<0.05),TG(mmol/L)1.99 ±0.42 and 1.45 ±0.32(P<0.01)and TC(mmol/L)6.11 ± 0.38 and 5.17 ±0.41(P<0.01),HDL(mmol/L)1.06 ±0.22 and 1.41 ±0.19(P<0.05),respectively.Before and after treat-ment WBCs(×103/L)were 6.03 ±0.42 and 5.52 ±0.37(P<0.01),serum LP-PLA2(mg/L)31.02 ±9.81 and 23.89 ±14.15 (P<0.01),respectively.Conclusion:Periodontal therapy can improve the blood lipid levels in patients with CP and MS.