Methotrexate (MTX)was linked covalently to monoclonal IgG antibody (AU_(14-1)) to a mouse uterine cervical cancer with the use of dextran T-40 as the intermediate carrier. The molar ratio of IgG: MTX was 1: 20 in the conjugate. The conjugate retained full antibody binding activity at the AU_(14-1) concentration of 3.75?10~(-8) M measured by an indirect membrane immunofluorescence assay. The in vitro experiments confirmed that the conjugate not only showed target-selective antitumor effecacy but also was proved to be more effective in inhibiting the growth of HeLa cells than the effect of U_(14) cells, so that it confirmed the "evolutionary antigen" theory. It is suggested that AU_(14-1)-Dex-MTX conjugate may have good potentiality in clinical application.

Vascular hyperpermeability is a cardinal feature of inflammation or burn in which an array of inflammatory mediators can cause such changes in the microvessels. The functional measures of microvascular exchange that represent the properties of microvascular walls are the permeability coefficients which have been reported from measurements on intact whole organisms (including human subjects), on perfused tissues and organs, on single perfused microvessels, and on monolayers of cultured microvascular endothelial cells. In this review, we summarize some experiments of vascular permeability in individually isolated perfused microvessels. [

Objective To study the value of sclerotherapy by indwelling intravenous cannula guided by color Doppler flow imaging in the treatment of thyroid cyst. Methods In the guidance of color Doppler flow imaging, the cyst was punctured with intravenous cannula. Then the cyst fluid was drawn out, the cyst cavity irrigated, and the absolute alcohol injected into. Results The treatment was completed successfully in 47 cases, without complications such as bleeding or nerve injuries. The cyst was wiped out within 1 month in 20 cases, within 3 months in 27 cases. All the 47 cases were followed for 6～12 months and no recurrence was seen.Conclusions Puncture guided by color Doppler flow imaging is safe and reliable. Use of absolute alcohol in sclerotherapy is effective.

Objective To investigate the relationships between the expressions of cidA and lrgA and the biofilm formation of Staphylococcus epidermidis clinical isolates. Methods Thirty-nine S. epidermidis clinical isolates were divided into biofilm formation group and non-biofilm formation group according to adhesion assays and icaA amplification, 20 strains for biofilm formation group and 19 strains for another group respectively. Amplify cidA and lrgA fragment with PCR, detect mRNA levels of cidA and lrgA with RT-PCR and then calculate the ratios of cid/lrg mRNA. Results All of the 39 isolates of S. epidermidis existed cidA and lrgA gene.Select 2 strains of S. epidermidis, Y36 and Y26, at random for detecting the cidA and lrgA mRNA levels of different phases, the highest level of cidA mRNA shows on 4 h's culture while lrgA shows on6 h. The relative expression levels of cidA of 39 isolates of S. epidermidis were 0.340 + 0.250 in biofilm formation group and 0. 406 +0. 408( P =0. 541 ) in non-biofilm formation group for 4 h culture, while the relative expression levels of lrgA were 0.325 ± 0.218 and 0.253 ± 0.211 respectively ( P = 0. 299). There were no significant differences between these two groups. The ratios of cid/lrg mRNA were 1.067 ±0.529 and 1.958 ±1.877 respectively, the difference of population distribution was significant( P = 0.001 ). Conclusion The expressions of cidA and lrgA of S. epidermidis clinical isolates may be time-limited. There were no significant differeces of expressions of cidA and lrgA between biofilm formation group and non-biofilm formation group. The ratios of cid/lrg mRNA maybe have some biological significance.

ObjectiveTo determine the ability of biofilm formation of Staphylococcus epidermidis isolates and study the influence of different extracellular DNA(eDNA) levels in S.epidermidis isolates on the ability of biofilm formation.MethodsDetect the biofilm-formation ability of 227 S.epidermidis isolates with adhesion assays,amplify the icaA gene fragment with PCR.The S.epidermidis isolates were divided into biofilm formation(BF) group and non-biofilm formation (NBF) group according to adhesion assays and icaA amplification.Detect eDNA levels of S.epidermidis in planktonic culture and microtitre plate static culture.The eDNA in S.epidermidis biofilms stained by AO-PI was observed by CLSM.Results26 isolates were positive in adhesion assays and 32 isolates existed icaA gene among 227 S.epidermidis isolates.Select 20 isolates with positive adhesion assays and positive icaA amplification for BF group.Select 19 isolates with negative adhesion assays and negative icaA amplification for NBF group.The eDNA levels were (32.2±10.1)μg/ml,(33.6±11.9) μg/ml,(34.3±10.0) μg/ml in BF group when cultured in planktonic condition for 2,4,6 h,while the eDNA levels in NBF group were (28.7±8.9) μg/ml,(31.5±11.7) μg/ml,(31.8±12.7) μg/ml respectively.There were no significant differences between the two groups for these three phases(P＞0.05),though the eDNA levels of BF group were higher than that of NBF group.The eDNA levels were (740.0±264.4) ng/A600 in BF group when cultured in static microtitre plate,higher than that of NBF group,(80.1 ±31.1) ng/A600,and the difference between these two groups was significant.The eDNA in BF isolate Y36 biofilms could be visualized by staining with AO and PI when observed by CLSM,while neither biofilm structure nor eDNA appeard when NBF isolate Y26 was cultured for 24 h.ConclusionS.epidermidis isolates have the ability of biofilm formation.eDNA is one of the important matrix components in the S.epidermidis biofilm-forming process.The eDNA of static culture in microtitre plate was more efficient than planktonic culture in the case of estimating the ability of biofilm formation of S.epidermidis.

ObjectiveTo study the influence of humanized management mode for infection consciousness and control effect of infection of medical staff. Methods90 medical staff from June 2010 to March 2011 were selected as research object,and they were randomly divided into the control group and the observation group with 45 cases in each group,then the infection consciousness,grasping situation of related knowledge and infection control effect of two groups before and after the management on the third and sixth month were analyzed and compared.Results The infection consciousness,grasping situation of related knowledge and infection control effect of the observation group after the management on the third and sixth month were all better than those of the control group,there were significant differences. ConclusionsThe humanized management mode plays an active role in improving infection related evaluation indices of hospital staff,and is also effective in control of infection.

This essay is to study on the effect of bee-pollen and its suitable dosage on improving sport performances. Fifty athletes were our experimental subjects. The athletes were divided into experiment group and control group. 14 objective indexes were measured before and after taking pollen at each of the 4 times within 3 months on both of experiment group and control group of athletes. The results showed that bee-pollen (15 g per day) improved the cardiac function, muscular strength and enduranco significantly.279 mice were experimental too, they were divided into experiment groups and control group. These animals were put in water for one hour per day in a period of 3 months. They were tested for pole climbing and exhausted swimming after taking pollen by the first, second and third months. Results showed that bee-pollen increased muscular strength and swimming time after taking the pollen at a large dosage.After taking the bee-pollen the body-weight remained somewhat unchanged.There is no observed side effect after taking bee-pollen by athletes.

Objective To explore the predictive effect of modified DECAF,DECAF,CAPS and APACHEⅡin the assessment of prognosis of AECOPD patients with respiratory failure. Methods Clinical data of 186 AECOPD cases complicated with respiratory failure were analyzed and four score modes were used within 24 hours of admission. Clinical endpoints were patients′ survival status 28 days after admission. The discriminative power of the four score modes was evaluated by the area under the receiver operating characteristic curve (AUC). Results AUC of the modified DECAF(0.777,95%CI:0.710-0.835) and DECAF (0.766,95%CI:0.699-0.825) for prognosis was significantly greater than that of CAPS(0.699 ,95%:0.628-0.764) and APACHEⅡ(0.715,95%:0.645-0.779). Conclusion The modified DECAF and DECAF have predictive values on assessing the prognosis of AECOPD patients with respiratory failure ,which are simple and efficient.

Objective To investigate the mechanism and the related factors of fetal hypoxia in intrahepatic cholestasis of pregnancy (ICP). Methods Cord blood total bile acids(TBA), hypoxanthine (HX), and endothelin (ET) concentrations were measured in 30 newborns of mothers with ICP and 30 infants of normal pregnancy as control, and the cord nucleated red blood cells (NRBC) were counted as well. Results (1)The mean cord arterious plasma HX concentration in hypoxic fetuses of mothers with ICP was significantly higher than those in fetuses without hypoxia of mothers with ICP and fetuses of normal pregnancy(18.68?15.73)?mol/L, (6.87? 2.82)?mol/L, (6.81?2.83)?mol/L( P 0.05) and the mean cord arterious plasma ET concentration (72.44?12.23) ng/L,(70.16?26.61) ng/L,(67.27?43.56) ng/L, P =0.910) among the three groups. (2) In ICP group, the mean cord serum TBA concentration in hypoxic fetuses was significantly higher than that in fetuses without hypoxia, while the latter was higher than that in control group (23.77?11.82)?mol/L,(14.86?5.46)?mol/L,(9.28?4.39)?mol/L;( P

Oxidation of white phosphor, us by peroxides to produce mono-alkyl phosphate in the alcoholic solution has been studied under non-and catalytic conditions. In this paper, the mechanism of the oxidation process was analyzed. The content of mono-alkyl phosphalte in the product is affected by different types of alcoholic solution and peroxide solvent. The result shows the availabulity of the following order for the activity of the peroxide solvent and alcoholic solutions-tert-butyl hydroperoxide>(di) benzoyl peroxide>hydroperoxide; methanol> n-butyl alcohol> phenol. Under optimized reaction conditions: n (a white phosphorus) : n( tert-butyl hydroperoxide) : n (methanol)= 1:10:12, reaction temperature 80 ℃, reaction time 2.5 h, products of 80.0% mono-alkyl phosphonates can be provided when white phosphorus undergoes oxidation by tert-butyl hydroperoxide in the methanol solutions. When Cu (I), and Cn(Ⅱ) complexes are used as catalysts, it is possible to significantly enhance the oxidation of white phosphorus with the increase in the reaction rate. Th'e order for activity of catalysts is Cu(acac)2>Cu (CH3COO)2>Cu (C3 H7COO)2:>CuI>CuCI2. Noticeably, with Cu(acac)2 as catalyst, the selectivity for mono-alkyl phusphonates can reach 95% under adequate reaction conditions.