After icv administration, the analgesic action of Hyp was found on the tail-flick and hot-plate tests. The analgesic action potency of icv Hyp was approximately equal to 1 ~20 of morphine and was about 100 times as potent as sc administration. No effect was seen on the analgesic action of Hyp after ip naloxone, the analgesic action of Hyp was no concern of L-enkephalin contents in discrete regions of rat brain either. But icv EGTA markedly enhanced while icv CaCl2 antagonized the analgesic action of Hyp. It was also found that calcium ion content in mice brain markedly reduced when the analgesic action of Hyp appeared. These results suggest that Hyp have significant central analgesic action and the central analgesic action of Hyp may relate to reducing of calcium ion in brain.

Hypoxia-ischemia induces an inflammatory and immune response in the nervous system that may be important for development of brain injury. Recent data implicate that cytokines, chemokines, adhesion molecules，are involved in the recruitment of inflammatory-immune cells. A cytokines which promote emigration of leukocytes from the vascular lumen into the injured brain tissue are produced at the site of incipient cerebral infarction. The expression of mRNA for alpha- and beta-chemokines preceded the appearance of immune cells suggesting that these molecules may have a role in the inflammatory response to insults. The humoral and cell-medated immunity under the conditions of focal brain ischemia was discussed. These results together indicate that cell-cell interaction by adhesion molecules and cytokines is an important component in the pathogenesis of ischemic cerebral diseases, especially at the acute phase.Recently accumulated data show that depleting the amount of circulating leukocytes or administering anti-inflammatory chemicals such as cytokine blocking agents, anti-adhesion molecule antibodies, and immunosuppressants effectively minimize the size of ischemia induced cerebral infarction.

Objective To compare the differences of personality in patients with depression between Uighurs and Han Chinese.Method Hospitalized depressed patients were selected including 44 cases of Uygur people,73 cases of Han people and Han people with normal control 41 cases.Using Minnesota Multiphasic Per-sonality Inventory(MMPI), Eysenck Personality Questionnai(EPQ) and Cattell's 16 Personality Factor (16PF) to make the survey.Results In MMPI : Uighur depression group's factors F,Hs, D,Hy,Pt,Pa and Sc's T score were all higher than 70,and Han depression group' s factors Hs, D,Hy,and T score of Pa were all higher than 70.Only F (76.98±16.01 vs 67.16±13.51, P＜0.01), Pt(72.09± 14.22 vs 66.82± 11.12, P＜0.05) and Sc (73.43± 13.02 vs 68.62± 11.14, P＜0.05) had statistically significant differences between the two groups.Comparing Han depression group with Han normal control group,only Pd score was not significantly different,the other nine scales were statistically significant (P＜0.01).In EPQ: comparing Uighur depression group with Han depression group,the 4 kinds of scale (extroversion, psychoticism, neuroticism and conceal) differences were not statistically significant (P＞0.05).And Han depression group compared with the Han control group, four scales were statistically significant differences (P＜0.01).In 16 PF: comparing Uighur depression group with Han depression group, only the wisdom of B (P＜0.01) and the independence of the Q2 (P＜0.05) between the two groups were statistically siguificant,other personality dimensions had no significant difference (P＞0.05).Comparing Han depression group with Han normal control group, the factors of gregariousness A, stability C, excitability D,perseverance G, boldness H, sensitivity I,skeptical L,anxiety and O,self-discipline Q3,tension Q4 (P＜0.0l) and experimental Q 1 (P＜0.05) differences were statistically significant, and the factors of Wisdom of B, aggressiveness E, fantasy M, sophisticated sex N, independence Q2 were not statistically significant (P＞0.05).Conclusion The personality model of depression between the Uygur and Han nationality has the consistency of national culture,and differences with normal people.Prompt Uygur and han depression may have a common characteristic of pathological personality model.Uighur and han ethnic differences in national culture and personality is the character of diversity,is not a Uighur and Han the pathological basis of personality of depression.

Objective To analyze the correlation between the expression of Abl interactor 1 (ABI1) and the clinicopathologic characteristics in colorectal adenocarcinoma tissues.Methods Immunohistochemistry was used to determine ABI1 expression in human colorectal adenocarcinoma tissues and matched adjacent tissues.A statistical analysis was used to determine the potential correlation between ABI1 expression and clinicopathological characteristics and prognosis.Results ABI1 is up-regulated in colorectal adenocarcinoma tissues compared with matched adjacent tissues (P =0.000),ABI1 expression is significantly correlated with infiltration (P =0.043) and differentiation (P =0.040),but not with sex,age,tumor location,clinical stage,lymph node metastasis and distant metastasis (P ＞0.05);It was also shown that ABI1 expression had a significant influence on prognosis (x2 =11.090,P =0.001).Multivariate analyses showed that high ABI1 expression is not an independent poor prognostic factor for overall survival (P =0.119).Conclusion ABI1 overexpression might act as pro-oncogene for patients with colorectal adenocarcinoma.

AIM To investigate actions of total paeony glycoside(TPG) on learning and memory capacity and related products of metabolism of senile mice induced by D galactose. METHODS The subacute senile mouse models induced by injection of D galactose subcutaneously were used. RESULTS TPG could improve the learning and memory capacity of model mice in shuttle test and enhance spatial resolution in water maze test. TPG not only reduced the content of monoamine oxidase(MAO) and inhibited the decrease of cholinesterase(CHE) significantly, but also lessened the level of malondialdehyde(MDA) and inhibited the decrease of superoxide dismutase(SOD) in cerebrum of model mice. TPG still promoted the recovery of lobar atrophy and hypoimmunity of senile mice remarkedly. CONCLUSION TPG possesses obvious effects of improvement on learning, spatial resolution and delaying senility.

AIM To establish mice hyperuricemia model METHOD Yeast extract paste 7 5～30 g?kg -1 was given to mice by ig once daily for 7,14,21,28 consecutive days, and detected the level of uric acid in serum RESULT The serum uric acid level in mice were (271 8?53 2),(215 4?31 5),(195 9?56 0),(142 1?30 7) ?mol?L -1 in 30 g?kg -1 group after administration 1,2,3,4 weeks;and (226 8?40 7),(148 67?30 4),(176 9?27 0),(119 3?27 4) ?mol?L -1 in 15 g?kg -1 group after administration 1,2,3,4 weeks;In 7 5 g?kg -1 group the serum uric acid level was (117 0?29 0) ?mol?L -1 after 1 week, respectively CONCLUSION Yeast extract paste (15～30) g?kg -1 given by ig could form mice hyperuricemia model, and the hyperuricemia could last 1～2 weeks

Objective To explore the mechanism of drug resistance of ovarian cancer cells to TRAIL-induced apoptosis.Methods We collected 3AO cells and CAOV3 cells,respectively,at 18,24,48 and 72 hour under 12.5,25,50 and 100 ng/mL concentrations of TRAIL.The rate of cell growth inhibition was checked by methyl thiazolyl tetrazolium (MTT)assay to evaluate the effect of TRAIL.Morphology of apoptotic cells was observed by TdT-mediated-dUTP nick end labeling (TUNEL).The apoptosis rate was detected by flow cytometry (FCM)and C-FLIP protein was determined by Western blotting.Results TRAIL inhibited the growth of 3AO and CAOV3 cells.The rate of growth inhibition at 24 hour was 28% in 3AO cells and 10% in CAOV3 cells.TRAIL induced apoptosis of cells.The apoptosis rate at 24 hour was 8.5% in 3AO cells,which was higher than 5.5% in CAOV3 cells.The expression level of C-FLIP protein was higher in CAOV3 cells than in 3AO cells.Conclusion C-FLIP protein is an important protein that regulates drug resistance of ovarian cancer cells to TRAIL-induced apoptosis.

To explore the relationship between the serum vascular endothelial growth factor (VEGF) level and the severity of obstructive sleep apnea hypopnea syndrome (OSAHS), the concentrations of serum VEGF in 40 OSAHS patients and 9 healthy controls were measured by using ELISA method. Meanwhile the correlation between the concentration of VEGF and parameters of polysomnography (PSG) was examined. Our results showed that the concentrations of VEGF were significantly higher in OSAHS patients with severe hypoxia (536.8+/-334.7 pg/mL) than in those with mild hypoxia (329.2+/-174.7 pg/mL) and healthy controls (272. 8+/-211.0 pg/mL) (P<0.05 for both). The concentrations of VEGF were also significantly higher in OSAHS patients with hypertension (484.5+/-261.4 pg/mL) than in those without hypertension (311.0+/-158.4 pg/mL) and healthy controls (272. 8+/-211.0 pg/mL) (P<0.05 for both). There was a positive correlation between the concentration of VEGF and the apnea hypopnea index (AHI) (r=0.34, P<0.05). It is concluded that the concentration of the serum VEGF is positively related to the severity of OSAHS. The elevated serum VEGF level may be involved in the pathogenesis of the complications of obstructive sleep apnea hypopnea syndrome.

Objective:To observe the effects of warming triple needling plus Chinese medication on inflammatory responses and daily functioning ability in patients with knee osteoarthritis(KOA)due to wind-cold-dampness Bi-impediment. Methods:A total of 101 patients with KOA due to wind-cold-dampness Bi-impediment were divided into an acupuncture-medication group and a Chinese medication group using the random number table method.Fifty cases in the Chinese medication group took oral Fang Feng Xi Bi Tang for treatment,and 51 cases in the acupuncture-medication group received additional warming triple needling therapy.The symptom score of traditional Chinese medicine(TCM),inflammatory factor levels,and motor function of the knee joint were compared before and after treatment.The clinical efficacy was also compared between the two groups after treatment. Results:Three cases in the acupuncture-medication group and 2 cases in the Chinese medication group dropped out during the study,and the two groups each had 48 cases being included in statistical analysis ultimately.The total effective rate was 95.8%in the acupuncture-medication group,higher than 79.2%in the Chinese medication group,and the between-group difference was statistically significant(P<0.05).After treatment,the TCM symptom score dropped in both groups(P<0.05)and was lower in the acupuncture-medication group than in the Chinese medication group(P<0.05).The levels of interleukin(IL)-6,tumor necrosis factor(TNF)-α,and IL-1β dropped after the intervention in both groups(P<0.05)and were lower in the acupuncture-medication group than in the Chinese medication group(P<0.05).The scores of knee pain intensity,knee joint stiffness,and diurnal functioning decreased after treatment in the two groups(P<0.05)and were lower in the acupuncture-medication group than in the Chinese medication group(P<0.05). Conclusion:Warming triple needling plus Fang Feng Xi Bi Tang can reduce inflammatory responses,improve daily functioning ability,and enhance the quality of life in patients with KOA due to wind-cold-dampness Bi-impediment.

Objective To investigate the expression of R-spondin3(RSPO3)in breast cancer and its prognostic value.Methods TIMER,The Cancer Genome Atlas(TCGA),Gene ExpressionProfilling In-teractive Analysis 2(GEPIA2),Human Protein Atlas(HPA)and other databases were used to analyze the differential expression of RSPO3 in breast cancer tissues and normal tissues around cancer,and analyze the correlation between its expression level and pathological parameters of breast cancer;then the expression and structural characteristics of RSPO3 transcripts in breast cancer were investigated by GEPIA2database;Kaplan-Meier Plotter was used to plot the prognosis survival curve between RSPO3 and breast cancer patients;gene ontology(GO)and kyoto encyclopedia of genes and ge-nomes(KEGG)were used for gene function enrichment analysis and metabolic pathway analysis;TIMER database was used to analyzed the relationship between RSPO3 expression level and immune cell infiltration in tumor microenvironment.Results RSPO3 was low ex-pressed in breast cancer,and its expression level was related to the age,tumor diameter and molecular type of breast cancer patients.The low expression of RSPO3 in breast cancer was related to poor prognosis of patients;the results of GO function enrichment analysis showed that RSPO3 interacting genes were mainly enriched in biological processes involving immune cells and their receptors;the results of im-mune cell infiltration analysis showed that the expression level of RSPO3 in breast cancer tissue was positively correlated with the infiltra-tion level of CD8+T cells,CD4+T cells,CD4+T cell memory,macrophages,B cells,etc.It was negatively correlated with the infiltra-tion level of Treg;the results of KEGG metabolic pathway analysis showed that RSPO3may be involved in Wnt/β-catenin pathway.Conclusion Low expression of RSPO3 in breast cancer is related to the poor prognosis of patients,which may be involved in the progres-sion of breast cancer through inhibition of immune cell infiltration and activation of Wnt/β-catenin pathway.