Pancreatic function diagnostant test (PFD) is performed with the oral administration of N-benzoyl-L-tryosyl-P-aminobenzoic acid (BT-PABA) .It is a specific test for the determination of the exocrine function of the pancreas. It can be more reliable if P-aminobenzoic acid absorbing test (PABA) is also performed to eliminate the intestinal, hepatic, and renal factors in PABA absorption and excretion.The authors used the combined method of PFD and PABA to study the pancreatic exicrine function in 32 cases of systemic lupus erythematosus (SLE) and 16 cases of dermatomyositis (DM) . It was found that lowed exocrine function of the pancreas occurred in 9.4% of SLE cases and in 18.8% of DM cases though no definite abdominal symptoms or signs could be observed in those cases. It is suggested that the combined PFD and PABA method be used in early SLE and DM cases to evaluate the pancreatic exocrine function.

Objective To investigate water treatment technology and pollution status of Giardia lamblia and Cryptosporidium parvum in resource water and finished water of the centralized water supply enterprises in Shenzhen,and to provide scientific basis for waterborn infectious disease control.Methods Field hygiene assessment were conducted on the resource water and water treatment technology of 19 water supply enterprises during May to July,2008.One water sample was collected from resource water and finished water in each centralized water supply enterprise respectively,and was detected for Giardia lamblia and Cryptosporidium parvum.Results The routine traditional water treatment technology including coagulation deposition,quartz sand filtration and chlorine disinfection was applied in all of the 19 centralized water supply enterprises.Giardia lamblia and Cryptosporidium parvum were not found in samples from the source water and finished water.Conclusion The centralized water supply system in Shenzhen isn't polluted by Giardia lamblia and Cryptosporidium parvum.

A(D、B were negative); As for TA100(+S9), only E was positive. Conclusion The mutagenicity of product water of six water works is relatively high,the type of mutagenicity is mainly code-shifting. Taking river water as the water source,using chlorine dioxide and active carbon filtration and no using pretreatment of chlorination may reduce the mutagenic organics.

Objective To explore the molecular mechanism for the self-limitation of adenoviral infections in human airway,the different impacts of adenovirus serotype 5 ( Ad5 ) and serotype 7 ( Ad7 ) infections on mucin 1 ( MUC1 ) expression in airway epithelial cells were preliminarily investigated.Methods The Ad5 and the Ad7 infection models were established in A549 cell line.qRT-PCR was performed to determine the transcription of MUC1 mRNA,and the expression of MUC1 in A549 cells infected by Ad5 or Ad7 was by detected Western blot.Results An up-regulation of the MUC1 mRNA level were observed after Ad5 infection for 6 h(P＜0.05 ),and the protein expression level of MUC1 increased in a time-dependent manner in 48 hours of Ad5 infection,while similar response of MUC1 mRNA was absent in Ad7 infection (6 h),even after prolonged (20 h) treatment ( P ＞ 0.05 ).Conclusion This study reveals an up-regulation of MUC1 expression as one of the early immune response to Ad5 infection,which implies that MUC1 may function fully or partially as an anti-inflammatory factor in the self-limitation effect of Ad5 infection.However,type7 adenoviral infection,may introduce a mechanism otherwise,but through MUC1.

Objective To explore the effects of the meteorologic factors on air pollutants and to establish a model of relationship between the meteorologic factors and concentration of air pollutants. Method The data of SO2,NO2 and PM10 concentration in the air and the related meteorologic factors (daily average,maximum and minimum atmospheric pressure and daily atmospheric pressure difference,daily average,maximum and minimum air temperature,monthly rainfall,daily average and minimum relative humidity and wind speed) from January 2002 to December 2007 were collected. The correlation between the air pollutant levels and related meteorologic factors was analyzed by the multiple regression models with SPSS 11.5 software and multiple regression equations were established. Results Minimum relative humidity was negatively correlated with the concentration of SO2 and NO2 in atmosphere . Daily minimum air temperature was negatively correlated with the concentration of NO2 in atmosphere. Daily average relative humidity and wind speed were negatively correlated with the concentration of PM10 in atmosphere. Daily atmospheric pressure difference was positively correlated with the concentration of PM10 in atmosphere. Multiple regression equations of the concentration of SO2,NO2 and PM10 in atmosphere and meteorologic factors have statistical significances. Conclusion The meteorologic factors have some effects on the air pollution levels. By using the correlation of meteorologic factors and the concentration of air pollutions,multiple regression equations can be established.

Objective To analyze time-space variation of air pollutants(SO2,NO2,PM10) and the correlation among them in inner region and outer region of Shenzhen from 2002 to 2007.Methods The daily concentration means of SO2,NO2 and PM10 during 2002 to 2007 from the nine environmental monitoring sites in Shenzhen were collected.The determination was conducted according to the Ambient Air Quality Criteria.Results From January to December,variation of air concentration of SO2,NO2 and PM10 met the "V" shape in inner region and outer region of Shenzhen in 2002-2007,which was descending from January to June and ascending from June to December.The level of the air pollutants in inner region and outer region was higher in fall and lower in summer.The air pollution level of the inner region was increasing from 2002 to 2004,then decreased in 2005,but increased again in 2006 and 2007.The tendency of the outer region was similar with the tendency of the inner region from 2002 to 2006,but the level of pollutants decreased in 2007.The air concentration of SO2 in outer region was higher than that in inner region in 2002-2007.The air concentration of NO2 and PM10 in outer region was higher than that in inner region in 2002 to 2006,but lower in 2007.The air concentration of SO2 in inner region and outer region in 2002-2007 was under the limit of grade 2 according to the GB 3095-1996,near to grade 1 in inner region.The air concentration of NO2 and PM10 was under the limit of grade 3 and grade 2 respectively.There was a close correlation among the levels of SO2,NO2,PM10 in Shenzhen.Conclusion The air quality in Shenzhen is better in China.The time-space variation of the air pollutants is obvious and a close correlation exists among the air pollutants.

Objective To investigate the water source pollution by parasitic protozoa in small towns and villages in Shenzhen,China,in order to present the data to establish the national hygienic standard for controlling parasitic protozoa pollution.Methods The samples of water source were collected from 8 village level water plants in Shenzhen.Giardia lamblia and Cryptosporidium were detected,according to the EPA methods,by filtering,rinsing,magnetic isolation and staining.Results Giardia lamblia was found in water sources of 6 water plants and Cryptosporidium was found in one water plant.Conclusion At present,some water sources of village level water plants where the reservoirs were used as the water source in Shenzhen have been polluted by Giardia lamblia and Cryptosporidium,that indicates the potentiality of the water-born-diseases in that areas.

Aim To explore the neuroprotective effects and possib le mechanism of bellidifolin on focal cerebral ischemia in rats.Methods Focal ce rebral ischemia was induced by permanent occlusion of the proximal portion of ri ght middle cerebral artery occlusion (MCAO). A neurological examination was perf ormed on each rat 4 hours,24 hours after ischemia by the method of Berderson .T he infarcted size was measured by 2,3,5-triphenrytetrazolium chloride(TTC) st aining technique at 24 hours post-ischemia.Effect of bellidifolin on intercellu lar adhesion molecule-1 (ICAM-1) and B lymphocyte myeloma (Bcl-2) immunoreact ive positive cells in peri-infarct region following ischemia and the histological neuronal changes were observed by means of immunohistochemical staining and H E staining.Result Bellidifolin significantly reduced infarc- ted size and improved the neurological deficit in rats .Bellidifolin produced effects of reduction in expression of ICAM-1 and upregulation of antia poptotic protein Bcl-2 on focal cerebral ischemia.Conclusion B ellidifolin has neuroprotective effects on focal cerebral ischemia in rats by or al administration.Mechanism of neuroprotective effects is related to downregulat ion of ICAM-1 and upregulation of antiapoptotic protein Bcl-2 on focal cerebr al ischemia.

AIM: To investigate the titer and avidity of serum immunoglobulin G (IgG) antibody for surface antigens of Porphyromonas gingivalis (P. gingivalis) in sera from patients with early-onset periodontitis (EOP) and evaluate the interrelationship of the antibody titer and avidity. METHODS: 15 patients with early-onset periodontitis, 16 patients with adult periodontitis(AP) and 14 periodontally healthy subjects(HS) participated in this study. Levels and avidities of IgG antibody against P.gingivalis lipopolysaccharide(LPS) were measured. Serum IgG titers against P.gingivalis antigens were measured by enzyme-linked immunosorbent assay (ELISA). IgG avidity was measured by diethylamine dissociation ELISA. RESULTS: Serum IgG antibody levels against P.gingivalis LPS in EOP and AP patients were significantly higher than that of healthy subjects (P0.05). Antibody avidities to P. gingivalis in EOP were significantly higher than that of AP patients and HS group (P0.05). CONCLUSION: These results indicate that there is no correlation between antibody levels and avidities in EOP and AP patients. IgG avidity can be considered as a valuable parameter in the diagnosis of EOP.

OSCE is an examination of objective evaluation of the clinical skills.This article introduced the development of OSCE at home and aboard,and compared the difference of the exam setting in America,England,Canada and China Medical University.The advantages and disadvantages of SP in OSCE were also analyzed.It was discussed that OSCE would be widely applied in China.