An improved method for determination of the total oxalate in spinach was proposed. Oxalate in the spinach was precipitated at alkaline in CaC2O4 form. The precipitate was obtained by centrifugation and then acidified by HCIO4. KMnO4 was added and absorptivity was determined at 525 nm at a fixed time. This method was accurate and simple. Organic impurities existed in spinach could be removed by ultrviolet ray. The range of determination was 2?g/ml-35?g/ml. The recovery rate was 93-105% and the coefficient of variation was less than 5.35%.

Objective To investigate the effects of Mahuang-Xixin-Fuzi decoction on the plasma levels of interlcukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in allergic rhinitis in rats. Methods Thirty-two Wistar rats were randomly divided into a control group, a model group, a chlorpheniramine group, and a Mahuang-Xixin-Fuzi decoction group, with 8 rats in each group. Allergic rhinitis was induced by ovalbumin sensitization. Rats in the chlorpheniramine and Mahuang-Xixin-Fuzi decoction groups received intragastric administration of 0.01%chlorpheniramine and Mahuang-Xixin-Fuzi decoction 0.4 g (twice daily for 21 days), respectively. The plasma levels of IL-6 and TNF-αwere measured with enzyme-linked immunosorbent assay. Results The frequencies of sneezing (2.4 ± 0.9/30 min vs.18.5 ± 3.6/30 min) and nasal rubbing (4.2 ± 1.5/30 min vs. 31.2 ± 3.7/30 min) in the Mahuang-Xixin-Fuzi decoction group were significantly lower than those in the model group (all P<0.05). The plasma levels of IL-6 (153.00 ± 13.87 pg/ml vs. 230.38 ± 33.92 pg/ml) and TNF-α(21.25 ± 4.62 pg/ml vs. 56.38 ± 7.93 pg/ml) in the Mahuang-Xixin-Fuzi decoction group were significantly lower than those in the model group (all P<0.05). Conclusions Mahuang-Xixin-Fuzi decoction can reduce the plasma levels of IL-6 and TNF-α, and alleviate symptoms in allergic rhinitis in rats.

Resistant starch is starch that escapes from digestion in small intestine and ferments in large intestine. Recent studies have demonstrated that resistant starch can decrease blood glucose, cholesterol, and triglyceride, enhance the sensitivity of insulin, and reduce body weight Therefore, it is useful for the prevention and treatment of diabetes. This article reviewed the effects of resistant starch on blood glucose, blood lipid and insulin resistance of diabetes.

This paper systematically summarizes treatment contraindicationsof the prescription Guizhi decotion, Mahuang decotion, Guizhi 2 yuebi 1 decotion, Daqinglongdecotion, Lingguizhugandecotion, Zhizichi decotion, Baihu decotion, Chengqi decoction, Zhuling decotion , Xiaochaihu decotion, Taohechengqi decotion, Guizhi adding Shaoyao decotion, Guizhi adding dahuang decotion, Sinidecotion, Huangqin decotion in Treatise on Febrile Disease. Doctors should pay attention to thesetreatment contraindications forbetter syndrome differentiational treatment, thus to improve its clinical efficacy.

OBJECTIVE: To investigate the effects of irbesartan and enalapril on the blood pressure and the expression of related plasma molecule-marker in SHR rats.METHODS: Forty SHR were randomly divided into irbesartan group(SHR-I),enalapril group(SHR-E),irbesartan-enalapril group(SHR-IE) and blank control group(SHR-C).Another ten WKY rats were assigned to normal group(WKY).The blood pressure and activities of sICAM-1 and PAI-1 were measured after 16-week's intragastric administration with corresponding drugs,and then the results were compared with those before experiment.RESULTS: As compared before treatment,there were significant differences in blood pressure in all drug-treated groups after treatment(P

Aim To provide experimental basis to study Human Cytomegalovirus(HCMV)infected disease and filtrate anti-virus drugs, HCMV acute interstitial pneumonia murine model was established. Methods Balb/c mice were infected with HCMV AD_ 169 strain. The rate of breath and weight of mice were observed, specific anti-HCMV antibody in serum was detected by ELISA, and viral isolation, Polymerase chain reaction(PCR) and pathological examination of lung tissue were performed aswell. Results Compared with the control group, the rate of breath of mice in the group infected with HCMV increased, while their weight decreased (P

Objective To explore the angiogensis in epithelial ovarian neoplasms and the relationship among angiogenesis and clinical stage, the histological type and pathological grade of epithelial ovarian carcinomas. Methods The microvessel density (MVD) was examined in 74 cases of epithelial ovarian neoplasm and 20 nomal samples by using immunohistochemical SP method. Results MVD increased gradually from benign to borderline malignancy and ovarian carcinomas, and MVD there was a significant difference in between the borderline malignancies and benign tumors or normal tissues(P

Objective:To study the expression of aromatasecy P450 and receptor of estrogen and progesterone in the tissue of endometrial hyperplasia and endometrial cancinoma,analysis the correlation of the endometrial carcinoma pathogenesis,clinical stage and cell differentiation.Methods:The immunohistochemiscal S-P method techniques were performed to study the expression of aromatasecy P450,estrogen receptor(ER) and progesterone receptor(PR) in the tissue of endometrium from 226 cases,including 42 cases of normal endometrium,52 cases of simple endometrial hyperplasia,38 cases of complex endometrial hyperplasia,36 cases of endometrial atypical hyperplasia and 58 cases of endometrial carcinoma.Results:There was no the positive expression in the normal endometrial tissues.But the positive expressions of aromatasecy P450 were found in the tissue of endometrial hyperplasia(simple endometrial hyperplasia,complex endometrial,atypical hyperplasia)and endometrial carcinoma.The rates of the positive expression were 40.4%,47.4%,69.4%,72.4% respectively.There were significant relationships among fifth groups(P

Objective To analyze the islet ? cell function and the insulin resistance of different components of impaired glucose regulation(IGR).Methods A total of 463 adults diagnosed as IGR and 200 adults diagnosed as NGT by 75g OGTT,were included for the analysis of the islet beta cell function and insulin resistance.Results In the IFG group,HOMA-IR was significantly higher than in NGT and IGT group(P

Objective To study the changes of the blood Platelet activating factor (PAF)levels in neonatal hypoxic ischemic encephalopathy(HIE),and explore the relationship between PAF levels and severity of brain damage. Method The levels of blood PAF and SOD were measured in neonates with HIE by radio immunoassay and compared with that of normal term neonates. Results Blood PAF levels were much higher in acute stage of HIE,and PAF levels increased with the severity of HIE.Blood SOD and platelets were much lower compared with PAF. PAF is negatively correlated to SOD and PLT.( r=-0.467 and r=-0.359,P