Objective To study the changes of the symptoms and quality of life in the patients after the transvaginal tape-obtura-tor(TVT-O) operation .Methods To retrospectively analyze the clinical data of 96 female patients with stress urinary incontinence (SUI) in our hospital from February 2007 to April 2012 .48 cases of retropubic tension free vaginal tape(TVT) were taken as the control group and 48 cases of TVT-O as the experimental group .The intraoperative general condition and postoperative symptoms changes and life quality were analyzed and compared between the two groups .Results The operation time ,blood loss and hospital stay time in the experimental group were significantly less than those in the control group (t=17 .061 ,17 .026 ,15 .465 ,P>0 .05) , the difference of operative effects between the two groups was not statistically significant (χ2 =0 .711 ,P>0 .05) .After treatment , all the patients′scores of quality of life were significantly improved compared with that before treatment (29 .1 ± 9 .6 ,30 .3 ± 9 .8 vs . 70 .4 ± 10 .2 ,68 .5 ± 11 .4 ,P<0 .05) ,but score differences between the two groups showed no statistical significance (P>0 .05) ,af-ter treatment ,the symptom scores and urinal pad use of two groups were obviously improved compared with those before treatment (13 .9 ± 2 .8 ,14 .1 ± 2 .5 vs .5 .1 ± 1 .2 ,5 .4 ± 1 .7 89 .6% ,93 .8% vs .50 .0% ,58 .3% ,P<0 .05) ,but the differences between the two groups were not statistically significant (P>0 .05) .Conclusion TVT-O can improve the symptoms and quality of life in the pa-tients with SUI .In the same time ,it has advantages of minimal invasion and low risk ,which is worth promoting ,but the surgeons need to accumulate more clinical experience .

Objective To study the role of schizophrenia health education in intervening relapse of schizophrenia. Method 78 patients with schizaphrenia have been intervened by useing SHE about their staying in hospital and conditions out of hospitals after having recovered for three years, then compared with 85 schizophrenics receiving no SHE. Result It was clear that general rate of relapse in the study group was significantly lower than that of the control group. The patients obey medication and recovery of social function in the study group were significanly better than that in the controlgroup. Conclusion The SHE plays an important role in intervening relapse of schizophrenia and improving complete rehabilitation of schizophrenic.

Objective To screen and identify the serum biomarker of anovulatory dysfunctional uterine bleeding (ADUB) , to determine the expression of biomarker protein in menses of ADUB pa-tients, and to investigate the relation between ADUB and the biomarker proteins. Methods Subjects included 128 ADUB patients and 93 age-matched controls( normal women ). Their serum and super-natant of mense were collected and stored for use at -80℃. The differential proteins in the serum of the 2 groups were detected by CM 10 and analyzed by Biomarker WizardTM3.2 software. Then, the differential proteins were identified by Trieine-SDS-PAGE gel separation, spectrometry identifica-tion, and immunoprecipitation. The expression of the protein identified above in the menses was test-ed by ELISA, RT-PCR, and Western blotting. SPSS 14.1 was applied for statistical analysis and chart drawing. Results Five differential protein peaks were screened and their peak values were 11.80, 13.59, 13.79, 13.85, and 14.20 km/z, respectively. The intensity of protein peak ( 11.80 km/z ) which was identified as serum amyploid protein A ( SAA ) of ADUB was significantly higher than that of the controls (P<0.05). While the intensity of protein peak (13.59 km/z) which was identified as vascular endothelial growth factor (VEGF) of ADUB was obviously lower than that of the controls (P<0.05). The intensity of protein peak 13.08, 13.85, and 14.20 was not different between the cases and controls. SAA expressed highly in the menses of ADUB but low in that of the controls. Conversely, VEGF expressed highly in the menses of the control but low in that of the ADUB. Conclusion Two biomarkers which might be related with ADUB have been correctly screened and identified as SAA and VEGF. It needs further study whether the increased expression of SAA and reduced expression of VEGF are the cause or result of ADUB.

In order to investigate the effect of anti-hepato-cellular carcinoma of HSV-tk suicide gene system, we constructed the HSV - tk recombinant retroviral vector pLXT. SMMC - 7721 hepatocellular carconoma cells more transfected with pLXT by lipofectin were obtained by subsequent G418 screen. 3H-TdR incorparation assay showed that HSV - tk/ACV had strong cytotoxic effect on HSV - tk gene transfected tumor cells. Lipofectin pLXT complex was directly injected into murine H22 hepatoma tissue, followed by delivery of ACV prodrup, and it was found that the tumor growth masses more greatly reduced. Animals treated with Liptk + ACV and tk + ACV had an apparent reduction of tumor size as compared with the animals in other six groups ( P

37 diabetic patients and 49 normal subjects were examined with Goldmann-Weekers dark adaptometer.The results showed that the absolute thresholds of dark adaptation in each group of diabetic patients were higher than those in normal controls. The more severe retinopathy was, the higher was the absolute threshold.The patients with more damaged visual acuity showed worse dark adaptation. However the dark adaptation in patients with good visual acuity was also abnormal.lt is suggested that dark adaptation is more sensitive than visual acuity in determining the visual function of diabetic patients.

Objective To explore clinical significance of C-reactive protein( CRP) and procalcitonin ( PCT) in chronic obstructive pulmonary disease( COPD) with acute exacerbation.Methods Included 120 COPD patients with acute exacerbations,and at the same time,according to the random number table method,120 COPD patients with stable were extracted, all respondents were given corresponding symptomatic support treatment, observation before treatment,after treatment,the 1d,7d,14d patients serum levels of CRP was associated with change PCT level. Results Compared with before treatment, after treatment, the serum levels of CRP of COPD patients with acute exacerbation of at 1d,7d,14d was associated with a decline in PCT level, different time points more statistically significant difference( all P<0.05);Compared with patients with stable,before treatment,after treatment,the 1d,7d serum levels of CRP[(12.32 ±6.59)mg/L,(9.05 ±3.31)mg/L,(7.31 ±2.31)mg/L,(4.45 ±1.54)mg/L]was associated with a high level of PCT[(0.34 ±0.05)μg/L,(0.26 ±0.04)μg/L,(0.18 ±0.04)μg/L,(0.10 ± 0.03)μg/L]were obvious,more statistically significant difference(all P<0.05);But after treatment,serum CRP was associated with differences in the level was no statistical comparison PCT significance ( P >0.05 ) .Conclusion COPD patients with acute exacerbation of serum CRP and early PCT significantly higher level,its level after treatment dropped significantly,joint observation CRP and serum PCT level can reflect COPD acute exacerbation issue of the inflammatory reaction.

OBJECTIVE:To study the impact of ethanol volume fraction on the extract rate of ginsenoside monomer. METH-ODS:Water and different volume fraction of ethanol solution(20%,50%,70%,95%,V/V)were used for ginseng extract. High performance liquid chromatography-quadrupole-time of flight-mass spectrometry (Q-TOF LC/MS) was applied to identify compo-nents attributable of each peak. Especially 16 kinds of main saponins were analyzed. RESULTS:The volume fraction of ethanol had a significant impact on extract rate of ginsenoside. When the volume fraction of ethanol was 70%,extract rate of ginsenoside was the highest;however,the differences were mainly caused by protopanaxatriol saponins. The yield of protopanaxadiol ginsen-osides was similar in different volume fraction of ethanol solution. CONCLUSIONS:When the volume fraction of ethanol is differ-ent,result is not only different in the total saponin,but also different in the proportion of the monomers.

The online teaching quality assessment system that was established by embedding the professional knowledge into the teaching of literature retrieval course, can help the students master the methods and skills to ac-cess and use the literature information they need and the basic knowledge of subjects in which they major .

OBJECTIVE:To study the antioxidant activity in vitro of neutral polysaccharides and its graded component from 3samples of white ginseng and red ginseng. METHODS:The decoction method was used to extract the crude polysaccharides fromwhite ginseng,100 ℃ and 120 ℃ processed red ginseng;the crude polysaccharides were further separated through ion exchangecolumn to extract neutral polysaccharides;Sephadex G-75 gels filter column was used to grade the neutral polysaccharides accordingto the molecular weight,antioxidant activity in vitro of 9 samples in 3 neutral polysaccharides and were detected by DPPH andOH free radical scavenging test and reduction capacity test(FRAP value),and vitamin C was used as positive control. RESULTS:The 3 neutral polysaccharides all obtained component Ⅰ and component Ⅱ after grading. Neutral polysaccharides and its gradedcomponent showed certain antioxidant activity in vitro in a certain concentration range,and increased by concentration increasing.The activity of neutral polysaccharides and component Ⅱ from 120 ℃ processed red ginseng was the strongest,of which 50% inhibitoryconcentration(IC50)on DPPH free radical was 0.258 g/L and 0.253 g/L,on OH free radical was 7.157 g/L and 6.845g/L,FRAP values were 2.8 and 3.0 mmol/L(when concentration was 1.2 g/L),respectively. CONCLUSIONS:The antioxidant activityin vitro from 120 ℃ processed red ginseng is higher than that of 100 ℃ processed red ginseng and white ginseng,in whichcomponent Ⅱ makes important contribution to the antioxidant activity.

Objective To analyze the changes of WBC classification,sugar and protein in cerebro-spinal fluid(CSF)and pathogenic bacteria of neonatal purulent meningitis.Methods Thirty-one neonates with bacterial meningitis in our department of neonatology from June 201 1 to June 2013 were enrolled,and the clinical features,pathogenic bacteria,laboratory examination of CSF were analyzed.Results Fever (90.3%),convulsions(67.7%)and changed consciousness(58.1 %)were common clinical symptoms.The incidences of other nervous system abnormal signs such as gastrointestinal dysfunction(25.8%),abnormal breathing(16.1 %),cervical resistance(16.1 %),bulging fontanel(9.7%)were lower.The Gram -negative bacteria was more than Gram -positive in both blood and CSF culture.The escherichia coli was the most common bacteria,with positive rate of 38.1 %(8 /21 )in blood culture and 55.5%(5 /9)in CSF culture.The germiculture positive rate in CSF was lower than in blood culture (29.0% vs.67.7%).Polymorphonuclear leukocyte(PMN)[(79.61 ±12.06)%]was the most predominant cell of the leukocyte classification in CSF within 1 week in all cases,PMN was still predominant in 1 to 2 weeks in 7 cases,while only 2 cases in 2 to 3 weeks still dominated by PMN,PMN was not the predominant cell 3 weeks later.Conclusion In the typi-cal neonatal purulent meningitis,PMN was the predominant cell in CSF within the first week,but the propor-tion of monocyte gradually increased and was dominant later.Escherichia coli was a common bacteria caused by this disease.