This study was aimed to observe the antibacterial activity and bactericidal action ofC.albicans in vitro, and the effects of curing monilial vaginitis mouse by extraction of globeflower residue fermentation (EGRF) in vivo.In vitrostudy, test tube method as well as plate method were used to determine the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) ofC.albicansrespectively.In vivo, mouse were devided into normal controlled group,C. albicans vaginitis model group (Model), Model + EGRF (40, 80, 160 mg?kg-1) group, and fluconazole group (20 mg?kg-1). All drugs were vaginal delivery once a day with continuous administration for seven days. Then vulva inflammation, negative rate of vaginal discharge, microbial load of vaginal lavage and the pathological changes of vaginal mucosa were observed. After the treatment of EGRF, the MIC and MBC of Candida albicans were 0.31 mg?mL-1 and 1.25 mg?mL-1, respectively, while the potency unit ratios between EGRF and fluconazole of MIC and MBC were 2 to 1 and 1 to 1, respectively. In comparison with Model, vulva inflammation of Model + EGRF gourp and fluconazole group was improved, whileC. albicanscount in vaginal secretions of these two groups were decreased, the overcast rate ofC. albicansof vaginal douche was increased, and pathological changes of vaginal mucosa were also improved in the two groups, which were in dose-dependent manners. And high dose Model + EGRF group was close to fluconazole group. In conclusion, EGRF had obvious inhibitory effect onC. albicans in vitro. It also had a better therapeutic effect onC. albicans vaginitis mouse.

Objective To explore the effectiveness of using middle-latency somatosensory evoked potentials (MLSEP) to predict the prognosis in patients with acute severe stroke. Methods MLSEP, shortlatency somatosensory evoked potentials (SLSEP), and Glasgow Coma Scale (GCS) were recorded in 70 acute severe supratentorial stroke patients within 1 week after onset. All patients were evaluated with modified Rankin Scale (mRS) and follow-up in 6 months after onset. SLSEP and MLSEP were recorded in 20 normal controls. The consistency between MLSEP, SLSEP, GCS and prognosis, as well as the prognostic authenticity of MLSEP, SLSEP, and GCS were analyzed. Results Bilateral N20, N35, and N60 exited in all normal controls. Some waves of MLSEP were absent in stroke patients, and the proportion of absent waves in ipsilateral MLSEP was higher than in contralateral MLSEP. The consistency between bilateral absence of N60 and unfavorable outcome ( Kappa = 0.828, P ＜ 0.01 ), and between bilateral absence of N60 and death ( Kappa = 0.686, P ＜ 0.01 ) was satisfactory. By using the prognostic authenticity analysis of predictors, the ipsilateral absence of N60 showed the highest sensitivity ( 100% ) for unfavorable outcome and death, which added 14.3% compared with the sensitivity of ipsilateral absence of N20 ( 85.7% ). Bilateral absence of N60 showed a high specificity of 100% for unfavorable outcome, which equaled bilateral absence of N20.However, it showed a lower specificity ( 82.9% ) for death, than bilateral absence of N20 (97.1% ).Conclusions MLSEP was able to reflect the degree of brain injury and showed higher sensitivity than SLSEP for predicting unfavorable outcomes. Therefore combined use of MLSEP and SLSEP in evaluating and predicting the outcomes in brain injuries is suggested.

Objective To explore the application of plastic pancreatic stents and/or nasal biliary drainage for choledocholithiasis patients having DSBC during Endoscopic Retrograde Cholangiopancreatography (ERCP).Methods Retrospective analysis on clinical data of 57 ERCP cases aiming at choledocholithiasis patients having DSBC from January 2010 to December 2015 has been carried out. According to the guide wire cannulation in an operation, patients are divided into three groups, i.e. plastic pancreatic stents group, nasal biliary drainage group, and plastic pancreatic stents + nasal biliary drainage group, so as to observe the success rate of operation and the occurrence rate of postoperative complications, such as pancreatitis or hyperamylasemia, as well as to compare the differences between the three groups.Results Out of the 57 patients receiving ERCP, 13 patients are in the plastic pancreatic stents group, with two successful operations (15.4%), one case of hyperamylasemia (7.7%), two cases of postoperative pancreatitis (15.4%), one case of fever (7.7%) and one case of hemorrhage (7.7%); 20 patients are in the nasal biliary drainage group, with 20 successful operations (100.0%), no occurrence of hyperamylasemia or postoperative pancreatitis or other complications including hemorrhage and fever; 24 patients are in the plastic pancreatic stents + nasal biliary drainage group, with 19 ERCP operations succeeded at the first attempt (79.2%) and 5 ERCP operations succeeded at the second try (20.8%), as well as 4 cases of hyperamylasemia (16.7%), 2 cases of hemorrhage (8.3%) , no occurrence of fever cases and postoperative pancreatitis. In comparison between the three groups, the occurrence of postoperative pancreatitis and successful rate of operation show a significant difference.Conclusion By adopting plastic pancreatic stents and/or nasal biliary drainage for patients having a dififcult selective biliary cannulation during ERCP, the success rate of operation can be improved, and the occurrence of pancreatitis can also be reduced.

Objective To analyze the clinic and imaging features of Klebsiella pneumoniae liver abscess in diabetes mellitus patients with different levels of HbA1c.Methods One hundred and forty-six diabetes patients with KPLA were divided on the basis of their levels of HbA1c into three groups:complete glycemic controlled (HbA1c ≤ 7％);good glycemic controlled (7％ ＜HbA1c≤9 ％) ≤ bad glycemic controlled (HbA1c＞ 9 ％).Compared the patients' characteristics,clinic features,imaging features and complications among each group.Results Compared with patients in groups of complete and good glycemic controlled,patients in group of bad glycemic controlled tend to have younger age at onset[(with an average age of (59.1 ± 13.8)years],longer hospital stay[(with an average stay of (23.1 ± 7.6) day] and more experience complications such as:hyperlipoidemia(49 cases,77.8％),chronic renal failure(12 cases,19.2 ％),life-threatening clinical crisis,and higher infection rate.Patients in bad glycemic controlled also had high risk of biliary pneumatosis(18 cases,28.6 ％),hepatic venous thrombosis (20 cases,31.8 ％) and gas-forming pyogenic liver abscess(26 cases,28.6 ％)(P＜0.05).Conclusion The complications of hepatic venous thrombosis,gas-forming and infection in diabetes patients with KPLA were associated with glycemic uncontrolled.

This paper expounds the concept and connotation of the nurse professionalism, introduces the re-search status of foreign nurses professionalism assessment tools,discusses the influence factors of nurse professional-ism,including:personal factors, organizational factors and occupational factors.It also puts forward following sugges-tions:constructing the nurse professionalism of theoretical system and evaluation methods, advocating the spirit of nurses professionalism cultivation,creating a health care professional environment,providing the reference the devel-opment of our country's theory and improve the nurse professionalism.

In order to explore the causes and nursing care of gastroesophageal reflux in patients with ceryical vertebra frac-ture and paraplegia,the clinical data of 79 patients were retrospectively analyzed. Among which,31 patients suffered from gas-troesophngeal reflux. The main causes of gastroesephageal reflux were improper body position,gastrointestinal dysfunction,con-sciousness disorders,lnappropriate nasngastric feeding,drng adverse reaction,inappropriate feeding time,lack of knowledge in nurse aids and family members. It is suggested to take proper body position,assess gastrointestinal functions,implement naso-gastric feeding correctly,observe patient carefully to detect gastroesophageal reflux as early as possible,as well as provide health education for the nurse aids and family members to prevent gastroesophageal reflux and complications.

Objective To explore the survival condition of hemodialysis patients in Ningxia Hui Autonomous Region. Methods Interviewed 15 patients with hemodialysis by applying Modified Grounded Theory Approach put forward by KinoSitaYasuHito,a Japanese scholar,the data of which were from qualitative inductive comparative analysis. Results As for the survival condition of hemodialysis patients in Ningxia,two relative themes were extracted, Helpless dialysis life Desire to return to society, the former contained 2 subtopics of hardship in life and susceptible mood, the latter contained 2 subtopics of cherish life and desire for care. Conclusion We should set up a social supporting system to improve the living condition of hemodialysis patients with the purpose of helping them return to the society.

Objective To explore the effect of team-based learning on the teaching and learning of Gynecologic and Obstetric Nursing.Method The team-based learning including preparation before class,and discussion,assessment and summarization in class was used in the teaching and learning of nursing of cervical cancer and the feedback from the students were collected.Results The average score of 71 students on the exam was 83.3±8.3.More than 85.9%of them agreed on the effectiveness of the team-based learning. Conclusion The team-based learning could improve students' self-learning and cultivate communicative skills and team spirits.

AIM:To study the reversal effect of a cyclosporin D analogue PSC833 on multidrug resistance of doxorubicin-resistant human myelogenous leukemia ( K562/DOX) cells.METHODS: The reversal effects of PSC833 on resistance to doxorubicin ( DOX)/vincristine ( VCR) in K562/DOX cells were observed by MTT assay.The cell cycle analysis was performed by flow cytometry.Annexin V/PI staining was used to identify PSC833-induced apoptosis in K562/DOX cells.These cells underwent incubation with DCFH-DA, JC-1 and Fluo-3/AM followed by flow cytometry for the measurement of reactive oxygen species ( ROS) , mitochondrial membrane potential (ΔΨm ) , and intracellular calcium, re-spectively.The protein levels of cytochrome C (Cyt C), Bcl-2, Bax, and cleaved caspase-3 were detected by Western blotting.RESULTS:The DOX/VCR-induced cytotoxicity was significantly potentiated by PSC833.PSC833 arrested the cells in G2/M phase and increased the apoptosis induced by DOX in K562/DOX cells.During the apoptosis, the level of ROS and intracellular calcium increased, while the level ofΔΨm decreased.Furthermore, the release of Cyt C, activation of caspase-3, up-regulation of Bax and down-regulation of Bcl-2 were observed in K562/DOX cells treated with PSC833 and DOX.CONCLUSION: The reversal effect of PSC833 on multidrug resistance in K562/DOX cells is associated with the induction of apoptosis through a mitochondria-dependent pathway.