Objective To study the clincial effects of of single Sialic four hexose sodium injection ganglio-side (GM1) in treating periventricular leukomalacia of premature infants. Methods 41 cases of periventricular ]eukomalacia patients were divided into GMI treatment group (n=22) and control group (n=19). The treatment group were given another GM1 treatment. Dynamic observation of changes in B-head information was conducted. Re-stilts 6 months after birth, 8 cases(36.4%) in treatment group and 13cases (68.4%)in control group occurred with ventricular dilatation (χ28.85, P<0.05). Nervous system examination revealed there were 5 cases (22.7%) in treatment group and 10 cases (52.6%) in control group with limited performance of the Board of mus-cle tone and abnormal movements (χ2 3.93, P<0.05); 4 cases in treatment group (18.2%) and 9 cases (47.4%) of control group had mild cognitive impairment and mental retardation according to nervous system sequel-ae(χ24.01,P<0.05). Conclusion GM1 can effectively treat periventricular leukomalacia and reduce abnormal movements.

Objective To investigate the short-term outcomes and oncological safety of laparoscopy resection for colorectal cancer. Methods Between January 2004 and March 2009, 35 patients with colorectal cancer underwent laparoscopic-assisted surgery in our hospital, among which 32 underwent radical resection, 1 underwent laparoscopic exploration, and 2 were converted to open surgery. These patients were allocated in the laparoscopic group. Same numbers of patients who underwent conventional open surgery during the same period of time were identified and allocated in the open group. Short-term data, including surgical time, intra-operative blood loss, intra-operative blood transfusion, length of incision, histopathological data, post-operative complications and post-operative functions, were collected and compared between the two groups. Results A total of 67 patients were enrolled (32 in both laparoscopy and open groups). The other 3 cases were analyzed separately (1 underwent laparoscopic exploration and 2 converted to open surgery). The two groups were well balanced as to age, gender, and TNM staging and location of tumour. Histopathologically, the laparoscopic approach was equal to conventional approach as regard to resection margin (distal margin, 5 cm vs 5 cm, P=0.664) and lymph node yield (7 vs 8, P=0.228). This study also showed a longer surgical time (250 min vs 180 min, P=0.006), but shorter length of incision (10 cm vs 20 cm, P<0.001), less demand of intra-operative blood transfusion (1 case vs 10 cases, P=0.003), reduced use of analgesics (12 cases vs 25 cases, P=0.004), shorter post-operative stay (9.5 days vs 11 days, P=0.008) and earlier recovery of bowel function of the laparoscopic group. There was no statistical difference of intra-operative blood loss (200 mL vs 200 mL, P=0.098), incidence of post-operative complications (8 cases vs 6 cases, P=0.545) and volume of post-operative negative drainage (507.5 mL vs 669.0 mL, P=0.475) between the two groups. Conclusions Though limited by a relatively small sample size, our study showed that laparoscopy approach for colorectal cancer is equal to open approach in terms of oncological safety and short-term outcome.

Objective To explore clinical value of detection of carcinoembryonic antigen(CEA) ,cytokeratin 19 fragment(CY-FRA21-1) and neuron specific enolization enzyme(NSE) in pleural effusion and serum during the differential diagnosis and patho-logical classification of lung cancer .Methods 180 patients with pleural effusion were divided into two groups ,one including 55 be-nign pleural effusion cases(benign pleural effusion group) and the other including 125 lung cancer cases(lung cancer group) .CEA , NSE and CYFRA21-1 in pleural effusion and serum of two groups were measured ,respectively ,and the measure results between two groups were conducted .Results CEA ,NSE and CYFRA21-1 levels in lung cancer group were significantly higher than those in benign pleural effusion group(P<0 .05) .CEA levels in pleural effusion and serum in lung adenocarcinoma group were significantly higher than those in benign pleural effusion group ,lung squamous carcinoma group and small cell lung cancer group(P<0 .05) .CY-FRA21-1 levels in pleural effusion and serum in lung squamous carcinoma group were significantly higher than those in benign pleu-ral effusion group ,lung adenocarcinoma group and small cell lung cancer group(P<0 .01) .NSE levels in pleural effusion and serum in small cell lung cancer group were significantly higher than those in benign pleural effusion group ,lung adenocarcinoma group and lung squamous carcinoma group(P< 0 .01) .The positive detection rate of CEA in lung adenocarcinoma group was significantly higher than that in lung squamous carcinoma group and small cell lung cancer group(P<0 .05) .The positive detection rate of CY-FRA21-1 in lung squamous carcinoma group was significantly higher than that in lung adenocarcinoma group and small cell lung cancer group(P<0 .05) .The positive detection rate of NSE in small cell lung cancer group was significantly higher than that in lung squamous carcinoma group and lung adenocarcinoma group(P<0 .01) .Conclusion CEA ,CYFRA21-1 and NSE in pleural effusion and serum are specific tumor markers of lung adenocarcinoma ,lung squamous carcinoma and small cell lung cancer ,respectively , which may have a certain clinical significance for the differential diagnosis and pathological classification of lung cancer .

Objective To establish a RP-HPLC method for quantitative determinat ion of the piperine in the Mongolian recipe of Haodaodun-10. Methods The determ ination was performed on Shim-pack VP-ODS(150 mm?4.6 mm,5 ?m)column and the mobile phase was a mixture of water and methanol (23 ∶77) with a flow rate of 0.8 mL/min at room temperature. The UV detection wavelength was 343 nm. Results The linear range of piperine was 0.5～5.0 ?g,r=0.999 8.The mean recovery was 9 8.1 %( RSD=1.76 %,n=5). Conclusion The method is simple,accurate and sensiti ve,and can be used for the quality control of Haodaodun-10.

Levels of adenosine, inosine and hypoxanthine in rabbit brain tissue du-ring acute cerebral hypoxia induced by hypotonic hypoxemia were increased much morethan that of the normal controls from 53.3?2.9, 115.6?11.8 and 186.5?10.3 to 816.4?59.0, 1049.7?37.5 and 704.4?55.3 ?M/g (X?SD) respectively (P

Objective To evaluate the clinical effect of thrombolysis combined with prostaglandin El (PGE1) in the treatment of patients with acute pulmonary thromboembolism (APTE).Methods According to the digital table,88 patients with APTE corresponding to thrombolytic therapy were randomly divided into the simply thrombolytic group(49 cases) and the joint PGE1 group(39 cases).The simply thrombolytic group were given conventional thrombolysis,and the joint PGE1 group were given thrombolysis combined PGE1 therapy.The levels of PaO2,PaCO2,pulmonary artery systolic pressure(PAsP),cardiac troponin I(cTNI),B-type natriuretic peptide(BNP) were monitored,and the clinical efficacy was evaluated.Results After the treatment,the levels of PAsP,cTNI,BNP of the joint PGE1 group were better than those of the simply thrombolytic group(t =2.173,2.349,2.495,all P ＜0.05).The clinical effective rate of the joint PGE1 group was 82.1％,which was significantly higher than that of the simply thrombolytic group (59.2 ％) (χ2 =5.339,P ＜ 0.05).The critical event occurrence of the joint PGE 1 group was 7.7 ％,which was significant lower than that of the simply thrombolytic group (24.5 ％) (χ2 =4.333,P ＜ 0.05).Conclusion Thrombolysis combined PGEI therapy may be more effective in reducing pulmonary artery pressure,protecting myocardia,improving the right heart function than only thrombolysis,and thus improve clinical symptoms and short-term prognosis in the APTE patients.

AIM:To establish an effective and convenient method for applying HPLC fingerprints to quality control in the production of Shanzhuang Jiangzhi Tablet(Semen cassiae,Fructus crataegi,and Folium nelumbinis).METHODS:DiamonsilTM C18(150 mm?4.6 mm,5 ?m) analytical column was used and eluted with a gradient program consisted of phase B(methanol) and phase D(1% phosphoric acid) and detected at 254 nm;the column temperature was 30 ℃.The flow rate was 1.0 mL/min.RESULTS:Ten batches of sample tablets were tested and gained HPLC fingerprint of the tablet containing 17 common peaks.CONCLUSION:This validated method is available for quality evaluation and quality control in Shanzhuang Jiangzhi Tablet.

Objective To investigate the diagnostic value of electrophysiological examination for tarsal tunnel syndrome (TTS).Methods Motor nerve conduction velocity (MCV),sensory nerve conduction velocity (SCV),F wave and electromyographic measurements were carried out with 26 clinically suspected TTS patients.Results Of the 26,22 patients were unilaterally affected and 4 were bilaterally affected,so 30 tarsal tunnels in total were affected.All received electrophysiological examination.Of the 30 affected tarsal tunnels,abnormal MCV was detected in 28 of the posterior tibial nerves (93.3％),while abnormal SCV was detected in 27 medial plantar nerves (90％) and 24 lateral plantar nerves (80％).Needle electromyography of 156 targeted foot muscles detected abnormal spontaneous potentials in 90 of them (57.7％),giant potential in 12 muscles (7.7％) and decreased recruitment in 76 (48.7％).Conclusions The distal posterior tibial nerve is impaired in TTS,presenting axonal damage in an electrophysiological examination.Such examinations should have an important role in the diagnosis and differential diagnosis of TTS.

The first permanent molar caries and current status of sealant of 7 - 12-year-old students were surveyed in Huairou district of Beijing.Based on the World Health Organization and national oral health survey standards,cluster and stratified random sampling methods were used.The prevalence rate of deciduous tooth caries was 17.34％ with a decayed/missing/filled teeth (dmft) index of 0.30.The caries rate of females was higher than that of males (20.60％ vs.14.08％ ).The incidence of mandible was higher than that of maxillary.And there was no significant difference around the jaw.The rate of fissure sealant was 51.36％.The current status of first permanent molar caries in this district was in accordance with the characteristics of general epidemiological caries disease.An overall policy of early discovery,early diagnosis and early treatment should be practiced.