Objective　To probe the curative effect of thiotepa and mitomycin C for the prevention of the relapse of pterygium after its operation.Methods　One hundred and eighty-two cases with pterygium were randomly divided into two groups:one group of thiotepa(158 cases with 169 eyes)and the other group of mitomycin C (24 cases with 24 eyes) and a comparative study was conducted.Thiotepa was used through dropping on the third day after the operation(1∶2 000),while MMC was used in the same way during the operation and on the third day after the operation respectively(0.2g*L-1).Results　In the group of thiotepa,the wounded skin on the cornea was repaired significantly more rapidly than that in the group of mitomycin C,and the rate of the relapse of pterygium was lower in the former group than that in the latter one.Conclusion　Applying thiotepa by dropping after the excision of pterygium is a safe,simple and effective supplementary measure for the prevention of pterygium relapse and also effective for recurring cases.

Objective To investigate the effect of channel density on the gating properties of Anoctamin 1(Ano1,TMEM16A)Ca2+?activated chlo?ride channel. Methods Ano1 expression plasmids were transiently transfected into HEK293 cells. High density and low density of Ano1 was ob?tained after expressing the protein for 24 h and 6 h,respectively. Electrophysiological recordings were performed in the whole?cell patch clamp con?figuration. The activation kinetics of current traces was fitted by exponentials. Results The current density was significantly higher in cells express?ing Ano1 for 24 h than those expressing Ano1 for 6 h(P<0.05). The activation of Ano1 current in cells with low density was well fit by a single expo?nential withτslow of 292.71±38.11 ms. The activation of Ano1 current in cells with high density was well fit by two exponentials withτfast of 47.78±4.58 ms andτslow of 385.74±71.44 ms. ANO1 current in cells with high density has a rapid active component(τfast)more than low density. There was no significantly different of theτslow between cells with high density and low density of Ano1(P>0.05). Conclusion Our findings suggested that chan?nel density regulates the gating of Ano1. High channel density promotes activation of Ano1.

Transmembrane protein 16A(TMEM16A)is a voltage-dependent calcium-activated chloride channel that is widely expressed in cancer cells.In a variety of cancer types,TMEM16A regulates the proliferation,invasion,and metastasis of cancer cells and is corre-lated with the prognosis of cancer under treatment.In recent years,TMEM16A and its inhibitors have been intensively studied in cancer treatment.This review summarizes relevant studies conducted over the past 10 years,aiming to provide a novel therapeutic strategy for the clinical application of TMEM16A inhibitors in future cancer therapy.

Objective:To investigate the effect of MCC950 (a NLRP3 inflammasome inhibitor) on cognitive impairment in mice with radiation-induced inflammatory brain injury.Methods:Mice were divided into normal (NS) group, whole body irradiation (IR) group and MCC950 intervention post irradiation (IR+ MCC950) group according to the random number table method, with 15 mice in each group. The mice in IR group and IR+ MCC950 group were irradiated with a single dose of 4.0 Gy. The radiation source was 137Cs and the dose rate was 1.118 Gy/min. The mice in NS group were not irradiated. Mice in IR+ MCC950 group were injected intraperitoneally with MCC950 once a day (10 mg/kg each time) from 3 weeks after irradiation. Behavioral tests such as new and old things recognition experiment and social cognition experiment were used to detect the cognitive function of mice. Immunohistochemistry was used to detect the expression of NeuN protein in CA3 area of mouse hippocampus. PCR and Western blot were used to detect the expression of NLRP3 inflammatory body related protein. Results:Compared with NS group, the short-term and long-term recognition index of new and old things in the IR group decreased significantly ( t=4.321, 5.473, P<0.01), and the social cognitive recognition index of the IR group also decreased significantly ( t=2.097, P<0.05). MCC950 treatment reversed the above changes (short-term and long-term new and old thing recognition test: t=5.860, 4.598, P<0.05; new and old position recognition test: t=3.040, P<0.05; social cognition test: t=4.021, P<0.01). The expression of NLRP3, Caspase-1, IL-1 β and IL-18 in mice hippocampus of the IR group was significantly higher than that of the control group ( t=2.699, 8.515, 3.340, 3.950, P<0.05). Compared with NS mice, radiation significantly increased the expressions of Bax, Caspase-3 and PARP1 in hippocampus ( t=3.887, 2.742, 3.287, P<0.05), while MCC950 significantly decreased the expressions of Bax, Caspase-3 and PARP1( t=2.852, 4.090, 9.614, P<0.05). Conclusions:NLRP3 inflammasome inhibitor MCC950 could alleviate radiation-induced cognitive impairment, which may be due to the inhibition of hippocampal inflammatory and neuronal death.