Objective To evaluate the therapeutic effect of glycyrrhizic acid ointment on chronic eczema in guinea pigs.Methods Totally,60 Kunming male mice,60 Kunming mice and 60 albino guinea pigs were respectively classified into 6 groups:model group,ointment base group,dexamethasone group,high-,medium-and low-dose glycyrrhizic acid group.To observe the effect of glycyrrhizic acid ointment on the swelling of mouse auricles,dexamethasone and different doses of glycyrrhizic acid ointment were topically applied to both auricles of male mice in corresponding groups followed by the establishement of mouse auricle swelling model induced by xylene.One and a half hours later,the mice were sacrificed,and the weight of swelling auricles was weighed.Scratching behavior was induced by dextran 40 in Kunming mice following the application of dexamethasone or glycyrrhizic acid ointment.The latent period and frequency of scraching were recorded for every Kunming mice.To establish a model of eczema,guinea pigs were sensitized with dinitrochlorobenzene (DNCB,5％) in the neck and back and challenged by DNCB (0.1％) on the inner surface of right ears once a week for 4 weeks;subsequently,both auricles were treated with dexamethasone or glycyrrhizic acid ointment once a day for 10 days.Then,the thickness of auricles of guinea pigs was measured,and skin tissue was obtained from the right ear of guinea pigs and subjected to a pathological analysis.Results The high- and medium-dose of glycyrrhizic acid ointment could suppress the xylene-induced swelling of mouse auricles (P ＜ 0.05 or P ＜ 0.01 ).The latent period of scratching could be lengthened by high dose of glycyrrhizic acid (P ＜ 0.05).The three doses of glycyrrhizic acid all reduced scratching frequency in Kunming mice (P ＜ 0.05 or P ＜ 0.01 ).Attenuated swelling of auricles (P ＜ 0.05 or P ＜ 0.01 ) and relieved pathological changes were observed in guinea pigs after treatment with glycyrrhizic acid ointment.Conclusion Glycyrrhizic acid ointment shows a therapeutic effect on chronic eczema in guinea pigs.

Objective To observe the clinical efficacy ofXing Nao Kai Qiao (brain awakening and orifice opening) Needling plus mountain-burning fire manipulation in treating post-stroke limb numbness.Method Eighty patients with post-stroke limb numbness were randomized into a treatment group and a control group, 40 cases in each group. The treatment group was intervened byXing Nao Kai Qiao needling plus mountain-burning fire manipulation, while the control group was byXing Nao Kai Qiao needling alone. The sensory function and motor function (Fugl-Meyer Assessment, FMA), National Institute of Health Stroke Scale (NIHSS), and Barthel Index (BI) were observed before and after intervention.Result The component scores of FMA (motor function, sensory function, range of motion, and joint pain intensity), NIHSS scores, and BI scores were significantly changed respectively after 1 and 2 treatment courses in both groups (P<0.05). The component scores of FMA, NIHSS scores, and BI scores after 2 treatment course were significantly different from that after 1 treatment course in both groups (P<0.05). After 2 treatment courses, the sensory function score, joint pain intensity in the treatment group were significantly different from that in the control group (P<0.05).ConclusionXing Nao Kai Qiaoneedling plus mountain-burning fire manipulation is an effective method in treating post-stroke limb numbness.

Objective To investigate the diagnostic value and clinical implications of low hemoglobin density(LHD%)in the pa-tients with cervical carcinoma complicating iron deficiency anemia(IDA).Methods The iron metabolism indexes and whole blood cell parameters were detected in 148 patients with cervical carcinomas.LHD% was calculated.The results were compared with that in the healthy group and the hysteromyoma complicating IDA group.Results LHD% was(63 ±30)% in the cervical carcinoma complicating IDA group and(32±15)% in the cervical carcinoma complicating iron deficient erythropoiesis(IDE)group,which were higher than(7±6)% in the healthy group,(22 ±21)% in the cervical carcinoma non-iron deficiency group and(16 ±10)% in the cervical carcinoma non-anemia group(P <0.05 ).And which had no statistical difference between the hysteromyoma complicating IDA group and the cervical carcinoma complicating IDA group(P >0.05 ).Conclusion The new RBC parameter LHD% has the reference value in the diagnosis of cervical carcinoma complicating IDA.The LHD% increase may prompt cervical carcinoma com-plicating IDA.

Objective To observe the clinical effect of treatment of duodenal trauma intubation duodenal decompression jejunostomy jointly by the duodenal catheter decompression.Methods 60 patients with duodenal trauma were randomly divided into two groups,30 cases were treated with simple duodenal catheter decompression as control group,30 cases treated with duodenal catheter decompression joint jejunum ostomy intubation duodenal decompression as the observation group,generally observed two groups of patients surgery and postoperative complications,in the day of surgery and postoperative 7d evaluation of patients with nutritional status.Results The surgical time and blood loss of the two groups had no statistically significant difference(P＞0.05),the observation group had no case of duodenal fistula and four cases in the control group(13.33％)occumed duodenal fistula.The difference between the two groups was statistically significant(x2=4.286,P＜0.05).Postoperative concurrent abdominal abscess,lung infection,wound infection had no statistically significant differences(P＞0.05).After observation group 7d body mass and right upper arm muscle circumference stability was significantly higher than that in the control group(x2=7.519,9.868,both P＜0.05).Conclusion Jejunostomy joint duodenal catheter decompression intubation duodenal decompression in treatment of duodenal trauma surgical decompression had significant effect,can significantly reduce the incidence of fistula,improve patients nutritional status,promote the duodenal wound healing.

Objective: To study the change of cyclic nucleotide in brain of rats induced by acute stress Method: The concentration of cAMP and cGMP in frontal lob, hippcampus, midbrain and hypothalamus were measured in 1 and 3 hours after stress respectively Results:The concentration of cGMP in hippcampus and hypothalamus were significantly higher than that of control group at 1 hour after stress (P

Air pollution and global warming are increasingly deteriorating. Large amounts of polyamides derived from fossil fuel sources are consumed around the world. Cadaverine is an important building monomer block of bio-based polyamides, thus biotechnological processes for these polymers possess enormous ecological and economical potential. Currently, the engineered strains for biological production of cadaverine are Corynebacterium glutamicum and Escherichia coli. We review here the latest research progress of biosynthesis of cadaverine including metabolism of cadaverine in microorganisms, key enzymes and transport proteins in cadaverine synthesis pathway, optimum pathways and cadaverine yields.
Biosynthetic Pathways ; Biotechnology ; Cadaverine ; biosynthesis ; Corynebacterium glutamicum ; metabolism ; Escherichia coli ; metabolism

Based oncompetence-basedphilosophy of education, core competences of Master of Nursing Specialist (MNS) postgraduates serve as a benchmark for the education quality. Furthermore, the evaluation of core competences is a key link. Our research mainly summarizes the evaluation methods of core competences of MNS at home and abroad, thus providing suggestions to the improvement of domestic core competences evaluation methods of MNS.

Purpose　The aim is to study the effects of solubl e chit osan on antitumor factors and NK cell activity in rats.Methods　The level of NO、TNF produced by peritoneal macrophages,IFN-γ production and NK ce ll activity of spleen was detected,after different concentrations of chitosan we re injected into the sarcoma 180 rats peritoneally.Results　Th e cont ents of NO、TNF、IFN-γ and NK cell activity in sacroma 180 rats were significa ntly higher in the chitosan groups than those in the control group (P＜0.01) .Conclusion　Chitosan could impove the immune function in rats .

Objective Through investigating the current situation of first national Standardized Medical Resident Training Bases construction in Xinjiang to provide the basis for the administrative department of public health to improve their resident training bases.Methods Selecting standardized training related personnel 133 people of 20 base hospital to conduct a questionnaire survey,and selecting the base hospit.al leaders,the management staff of functional departments,teachers and residents,a total of 254 people were given in-depth interviews.We conducted data collection mainly around the basic situation of the base hospitals and departments,to understand the basic situation,difficulties and problems of the standardized training of resident doctors in the base hospitals,and suggestions and opinions on the training work.We used x2 test,Wilcoxon rank test to make statistical analysis.Results Basic conditions of the hospital was not balanced,70％ of the base hospital library number was 10 thousand ～5 million,75％ of the base hospital computer had a volume of less than or equal to 30.There was statistical significance in the difference of the rate of operation between the cases and the skills in the southem/northeru region teaching/non professional teaching base hospital.Teacher level was not balanced,and only 5 hospital teacher training rate was greater than or equal to 80％,which met the national requirements.Enrollment of each department had a rising trend,including the department of pediatrics,psychiatry and other major shortage of profes-sional year by year,the increase in the proportion of 1 ～2 times.National financial aid was implemented,but lacked local financial input.Conclusion The balanced development of base construction,the continuous improvement of system construction and organization management,the improvement of the teachers' teaching and training quality,the improvement of the resident treatment and striving for more national and local financial allocation will be the focus of the construction and development of Xinjiang resident standardized training base.

Objective To investigate the intra-splenic blood cell count of posthepatitic cirrhotic portal hypertension,and compare it with patients' peripheral blood cell count to explore the role the spleen plays in peripheral cytopenia often seen in posthepatitic cirrhotic portal hypertension.Methods A prospective study was made on 15 cases with post hepatitis B cirrhotic portal hypertension undergoing splenectomy.Intrasplenic blood was sampled from upper pole,hilus (central pole),and lower pole of the spleen respectively for blood cell count.Results were compared with that of preoperative peripheral blood.Results There were significant statistical differences in the WBC count between splenic blood and peripheral blood,(11.20 ± 4.73) × 109/L vs.(4.06 ± 1.75) × 109/L,t =5.05,P ＜ 0.05),and in PLT count,(182.45±66.57) × 109/L vs.(63.54 ±28.40) × 109/L,t =7.285,P ＜0.05.There was no differences in the RBC count,(3.55 ± 0.94) × 1012/L vs.(3.01 ± 0.62) × 1012/L,t =1.874,P ＞ 0.05.Positive correlations were found between splenic PLT count and peripheral PLT count (r =0.610,P ＜0.05).Conclusions In posthepatitic B cirrhotic portal hypertension patients the intra-megalosplenic PLT and WBC count are significantly higher than that in peripheral blood.Megalosplenic PLT count correlates positively with peripheral PLT count.